Human Semaphorin-3B ELISA Kit | SEMA3B elisa kit

Human Semaphorin-3B ELISA Kit

Cat. Num. AAA761222

• Gene Names: SEMA3B; SemA; SEMA5; SEMAA; semaV; LUCA-1
• Reactivity: Human
• Synonyms: Semaphorin-3B; Human Semaphorin-3B ELISA Kit; SEMA3B/LUCA-1/SEMA3B/SEMA5/SEMAA/SEMAV/semaphorin 3B/LUCA-1/SemA/Sema A (V)/sema domain; immunoglobulin domain (Ig); short basic domain; secreted/Sema V/sema5/SEMA5/SEMAA/semaphorin A/semaphorin V/semaphorin-3B/Semaphorin-V/semaphorin-V/semaV/Sema A (V)/Semaphorin-V (Sema V); SEMA3B elisa kit

• Reactivity: Human
• Sequence Length: 749

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 1.563-100ng/ml
• Sensitivity: 0.938ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for SEMA3B elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Human SEMA3B antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human SEMA3B antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human SEMA3B amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human SEMA3B can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 586798180
• NCBI GeneID: 7869
• NCBI Accession #: NP_001276989.1
• NCBI GenBank Nucleotide #: NM_001290060.1
• Molecular Weight: 83,035 Da
• NCBI Official Full Name: semaphorin-3B isoform 1
• NCBI Official Synonym Full Names: semaphorin 3B
• NCBI Official Symbol: SEMA3B
• NCBI Official Synonym Symbols: SemA; SEMA5; SEMAA; semaV; LUCA-1
• NCBI Protein Information: semaphorin-3B
• UniProt Protein Name: Semaphorin-3B
• Protein Family: Semaphorin
• UniProt Gene Name: SEMA3B
• UniProt Synonym Gene Names: SEMA5; SEMAA; Sema V
• UniProt Entry Name: SEM3B_HUMAN

NCBI Description

The protein encoded by this gene belongs to the class-3 semaphorin/collapsin family, whose members function in growth cone guidance during neuronal development. This family member inhibits axonal extension and has been shown to act as a tumor suppressor by inducing apoptosis. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Feb 2014]

Uniprot Description

SEMA3B: Inhibits axonal extension by providing local signals to specify territories inaccessible for growing axons. Expressed abundantly but differentially in a variety of neural and nonneural tissues. Belongs to the semaphorin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell development/differentiation; Secreted, signal peptide; Secreted; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 3p21.3

Cellular Component: endoplasmic reticulum; extracellular space

Molecular Function: chemorepellent activity; semaphorin receptor binding

Biological Process: axon guidance; cell-cell signaling; negative chemotaxis; negative regulation of axon extension involved in axon guidance; neural crest cell migration; positive regulation of cell migration