CD162 recombinant protein

Recombinant Human CD162 Protein

Cat. Num. AAA8304896

• Gene Names: SELPLG; CLA; CD162; PSGL1; PSGL-1
• Applications: ELISA, Western Blot, SDS-Page
• Purity: >95% as determined by reducing SDS-PAGE
• Synonyms: CD162; Recombinant Human CD162 Protein; P-selectin glycoprotein ligand 1; PSGL-1; Selectin P ligand; CD162 recombinant protein

• Host: Human Cells
• Purity/Purification: >95% as determined by reducing SDS-PAGE
• Form/Format: Lyophilized from a 0.2 mum filtered solution of 20mM Tris, 150mM NaCl, pH7.5.
• Sequence: TEYEYLDYDF LPETEPPEML RNSTDTTPLT GPGTPESTTV EPAARRSTGL DAGGAVTELT TELANMGNLS TDSAAMEIQT TQPAATEAQT TQPVPTEAQT TPLAATEAQT TRLTATEAQT TPLAATEAQT TPPAATEAQT TQPTGLEAQT TAPAAMEAQT TAPAAMEAQT TPPAAMEAQT TQTTAMEAQT TAPEATEAQT TQPTATEAQT TPLAAMEALS TEPSATEALS MEPTTKRGLF IPFSVSSVTH KGIEGRMDPK SCDKTHTCPP CPAPELLGGP SVFLFPPKPK DTLMISRTPE VTCVVVDVSH EDPEVKFNWY VDGVEVHNAK TKPREEQYNS TYRVVSVLTV LHQDWLNGKE YKCKVSNKAL PAPIEKTISK AKGQPREPQV YTLPPSRDEL TKNQVSLTCL VKGFYPSDIA VEWESNGQPE NNYKTTPPVL DSDGSFFLYS KLTVDKSRWQ QGNVFSCSVM HEALHNHYTQ KSLSLSPGK
• Sequence Length: 428

• Applicable Applications for CD162 recombinant protein: ELISA (EIA), Western Blot (WB), SDS-PAGE
• Endotoxin: Less than 0.1 ng/ug (1 IEU/ug) as determined by LAL test.
• Preparation and Storage: Lyophilized protein should be stored at -20 degree C, though stable at room temperature for 3 weeks.; Reconstituted protein solution can be stored at 2-8 degree C for 2-7 days. Aliquots of reconstituted samples are stable at -20 degree C for 3 months.

Related Product Information for CD162 recombinant protein

Recombinant Human CD162 Protein is produced by mammalian expression system and the target gene encoding Thr44-Gly295 is expressed with a Fc tag at the C-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 331284238
• NCBI GeneID: 6404
• NCBI Accession #: NP_001193538.1
• NCBI GenBank Nucleotide #: NP_001193538.1
• UniProt Accession #: Q14242
• NCBI Official Full Name: P-selectin glycoprotein ligand 1 isoform 1
• NCBI Official Synonym Full Names: selectin P ligand
• NCBI Official Symbol: SELPLG
• NCBI Official Synonym Symbols: CLA; CD162; PSGL1; PSGL-1
• NCBI Protein Information: P-selectin glycoprotein ligand 1
• UniProt Protein Name: P-selectin glycoprotein ligand 1
• UniProt Gene Name: SELPLG
• UniProt Synonym Gene Names: PSGL-1
• UniProt Entry Name: SELPL_HUMAN

NCBI Description

This gene encodes a glycoprotein that functions as a high affinity counter-receptor for the cell adhesion molecules P-, E- and L- selectin expressed on myeloid cells and stimulated T lymphocytes. As such, this protein plays a critical role in leukocyte trafficking during inflammation by tethering of leukocytes to activated platelets or endothelia expressing selectins. This protein requires two post-translational modifications, tyrosine sulfation and the addition of the sialyl Lewis x tetrasaccharide (sLex) to its O-linked glycans, for its high-affinity binding activity. Aberrant expression of this gene and polymorphisms in this gene are associated with defects in the innate and adaptive immune response. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Apr 2011]

Uniprot Description

SELPLG: A SLe(x)-type glycan, which through high affinity, calcium-dependent interactions with E-, P- and L-selectins, mediates rapid rolling of leukocytes over vascular surfaces during the initial steps in inflammation. PSGL1 is critical for the initial leukocyte capture.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 12q24

Cellular Component: membrane; integral to plasma membrane; plasma membrane; integral to membrane

Molecular Function: protein binding; receptor binding

Biological Process: blood coagulation; cell adhesion; leukocyte tethering or rolling; leukocyte adhesive activation; leukocyte migration