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6 results for "probable-phosphatidylinositol-4-phosphate-5-kinase" - showing 1-6
Immunofluorescence (IF)
(Fluorescent image of U251 cells stained with PI3KC3 (S34) antibody. U251 cells were treated with Chloroquine (50 uM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with MBS9204783 PI3KC3 (S34) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). PI3KC3 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.)
PI3KC3, Polyclonal Antibody (Cat# AAA9204783)
Full Name
PI3KC3 Antibody (S34)
Gene Names
PIK3C3; VPS34; Vps34; hVps34
Reactivity
Human (Predicted Reactivity: Mouse, Rat, Pig, Xenopus)
Applications
IF, EIA, WB, IHC
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Probable phosphatidylinositol 4-phosphate 5-kinase MSS4 (MSS4), Recombinant Protein (Cat# AAA1253602)
Full Name
Recombinant Saccharomyces cerevisiae Probable phosphatidylinositol 4-phosphate 5-kinase MSS4 (MSS4) , partial
Purity
Greater or equal to 85% purity as determined by SDS-PAGE.
Western Blot (WB)
(Western blot analysis of PI 3 Kinase Class 3 expression in 293T cell lysate (MBS179834).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PIK3C3 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PIK3C3 )
PI 3 Kinase Class 3, Monoclonal Antibody (Cat# AAA179834)
Full Name
Anti-PI 3 Kinase Class 3 Rabbit Monoclonal Antibody
Gene Names
PIK3C3; VPS34; Vps34; hVps34
Reactivity
Human, Mouse, Rat
Applications
IHC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
MSS4, Polyclonal Antibody (Cat# AAA7155555)
Full Name
Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508/S288c)(Baker's yeast) MSS4 Polyclonal Antibody
Reactivity
Saccharomyces cerevisiae (strain ATCC 204508/S288c)(Baker's yeast)
Applications
EIA, Western Blot, ensure identification of antigen
Purity
Protein A/G
Centaurin alpha 1, Polyclonal Antibody (Cat# AAA9629718)
Full Name
Centaurin alpha 1 Antibody
Gene Names
ADAP1; GCS1L; CENTA1; p42IP4
Reactivity
Human, Mouse, Rat
Applications
EIA, peptide
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
PLEKHO1, Polyclonal Antibody (Cat# AAA9630099)
Full Name
PLEKHO1 Antibody
Gene Names
PLEKHO1; OC120; CKIP-1
Reactivity
Human, Mouse, Rat
Applications
EIA, peptide
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
