NFKBIL2, NT (TONSL, IKBR, NFKBIL2, Tonsoku-like protein, Inhibitor of kappa B-related protein, NF-kappa-B inhibitor-like protein 2, Nuclear factor of kappa light polypeptide gene enhancer in B Cells inhibitor-like 2) (AP)
HINT1 (Histidine Triad Nucleotide-binding Protein 1, Adenosine 5'-monophosphoramidase, FLJ30414, FLJ32340, HINT, PKCI1, PKCI-1, PRKCNH1, Protein kinase C inhibitor 1, Protein Kinase C-interacting protein 1) (AP)
Western Blot (WB) (Western Blot detection against Immunogen (36.52kD).)
Testing Data (Detection limit for recombinant GST tagged CSTA is 0.1ng/ml as a capture antibody.)
Western Blot (WB) (Western blot analysis of Focal Adhesion Kinase (pY861) expression in HUVEC (A); HeLa (B) whole cell lysates.)
Western Blot (WB) (Western blot analysis of PLA2G4A (pS505) expression in HeLa lambda protein phosphatase-treated (A); NIH3T3 lambda protein phosphatase-treated (B) whole cell lysates.)
Western Blot (WB) (Mouse Cst3 Antibody (Center) western blot analysis in mouse spleen tissue lysates (35ug/lane).This demonstrates the Cst3 antibody detected the Cst3 protein (arrow).)
Western Blot (WB) (Western blot analysis of PHLPP expression in HEK293T (A); SP2/0 (B); PC12 (C) whole cell lysates.)
Western Blot (WB) (Western blot analysis of SHIP1 expression in THP1 (A); NAMALWA (B); Ramos (C) whole cell lysates.)
Dot Blot (DB) (Dot blot analysis of Mouse p27Kip1 Antibody (Phospho T197) Phospho-specific Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.6ug per ml.)
Western Blot (WB) (Western Blot analysis of fetal speen Lysate with anti-DUSP14 antibody)
Immunohistochemistry (IHC) (Immunohistochemical staining of formalin-fixed paraffin-embedded fetal testis showing cytoplasmic and nuclear staining with anti-DUSP14 antibody at a dilution of 1/100)
Western Blot (WB) (Western blot analysis of extracts of various cell lines, using NFKBIB antibody.)
Immunofluorescence (IF) (Immunofluorescence analysis of A549 cell using NFKBIB antibody. Blue: DAPI for nuclear staining.)
Western Blot (WB) (Mouse Cdkn1b Antibody (N-term) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the Mouse Cdkn1b antibody detected the Mouse Cdkn1b protein (arrow).)
Western Blot (WB) (Western Blot analysis of SERPINB10 expression in transfected 293T cell line by SERPINB10 monoclonal antibody (M09), clone 2B8.Lane 1: SERPINB10 transfected lysate (Predicted MW: 45.4 KDa).Lane 2: Non-transfected lysate.)
Western Blot (WB) (Western blot analysis of CDC25C expression in A431 (A); MCF7 (B); mouse kidney (C); rat heart (D) whole cell lysates.)
Testing Data (Detection limit for recombinant GST tagged SERPINA3 is approximately 3ng/ml as a capture antibody.)
Immunohistochemistry (IHC) (Immunoperoxidase of monoclonal antibody to SERPINA3 on formalin-fixed paraffin-embedded human kidney. [antibody concentration 3 ug/ml])
Immunohistochemistry (IHC) (Immunoperoxidase of monoclonal antibody to SERPINA3 on formalin-fixed paraffin-embedded human kidney. [antibody concentration 3 ug/ml])
Immunofluorescence (IF) (Immunofluorescence of monoclonal antibody to SERPINA3 on HeLa cell. [antibody concentration 10 ug/ml])
Immunofluorescence (IF) (Immunofluorescence of monoclonal antibody to SERPINA3 on HeLa cell. [antibody concentration 10 ug/ml])
Western Blot (WB) (SERPINA10 rabbit polyclonal antibody. Western Blot analysis of SERPINA10 expression in human kidney.)
Western Blot (WB) (Western Blot analysis of SERPINA10 expression in transfected 293T cell line by SERPINA10 polyclonal antibody. Lane 1: SERPINA10 transfected lysate (50.7kD). Lane 2: Non-transfected lysate.)
Western Blot (WB) (Western blot analysis of extracts from 293 cells, using Phospho-NFKBIE-S22 antibody.)
Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Phospho-NFKBIE-S22 antibody.)
Western Blot (WB) (Western blot analysis of 30 ug of whole cell lysate (A: A431) using a 7.5% SDS PAGE gel and PPP2R5B antibody at a dilution of 1:1000)
Western Blot (WB) (LXN monoclonal antibody (M01), clone 8H5. Western Blot analysis of LXN expression in MCF-7.)
Western Blot (WB) (Western Blot analysis of LXN expression in transfected 293T cell line by LXN monoclonal antibody (M01), clone 8H5.Lane 1: LXN transfected lysate (Predicted MW: 25.8 KDa).Lane 2: Non-transfected lysate.)
Testing Data (Detection limit for recombinant GST tagged LXN is 0.1 ng/ml as a capture antibody.)
Western Blot (WB) (Western Blot detection against Immunogen (67.87KD).)
Western Blot (WB) (SERPINA6 monoclonal antibody Western Blot analysis of SERPINA6 expression in HL-60.)
Testing Data (Detection limit for recombinant GST tagged SERPINA6 is ~0.03ng/ml as a capture antibody.)
Western Blot (WB) (SERPINB11 Antibody (Center) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the SERPINB11 antibody detected the SERPINB11 protein (arrow).)
Western Blot (WB) (Western blot analysis of extracts of various cell lines, using SERPINA1 antibody.)
Western Blot (WB) (CDKN3 Antibody (N-term) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the CDKN3 antibody detected the CDKN3 protein (arrow).)
Western Blot (WB) (Mouse Cdkn2a Antibody (Center) western blot analysis in mouse spleen tissue lysates (35ug/lane).This demonstrates the Cdkn2a antibody detected the Cdkn2a protein (arrow).)
Testing Data (HeLa cells were grown to 90% confluency and stimulated with 30 ng/mL TNF-)
ELISA (EIA) (Enzyme-Linked Immunosorbent Assay (ELISA) for immunogen phosphor-peptide (left) and non-phospho peptide (right), using Anti-FAK (Phospho-Tyr397) Antibody.)
Dot Blot (DB) (Dot blot analysis of anti-Phospho-NFKBIA (Ser32) Antibody Phospho-specific Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.)
Western Blot (WB) (NFKBIL2 Antibody (N-term) western blot analysis in MDA-MB453 cell line lysates (35ug/lane).This demonstrates the NFKBIL2 antibody detected the NFKBIL2 protein (arrow).)
Dot Blot (DB) (Dot blot analysis of Mouse p21Cip1 Antibody (Phospho S125) Phospho-specific Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.6ug per ml.)
Western Blot (WB) (Western Blot detection against Immunogen (39.6kD).)
Western Blot (WB) (Western Blot analysis of HINT1 expression in transfected 293T cell line by HINT1 monoclonal antibody. Lane 1: HINT1 transfected lysate (13.8kD). Lane 2: Non-transfected lysate.)
Immunohistochemistry (IHC) (Immunoperoxidase of monoclonal antibody to HINT1 on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 3ug/ml])
Western Blot (WB) (Western blot analysis of extracts of various cell lines, using PEBP1 antibody.)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human thyroid cancer using PEBP1 antibody at dilution of 1:200 (400x lens).)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded rat liver using PEBP1 antibody at dilution of 1:200 (200x lens).)
Immunofluorescence (IF) (Immunofluorescence analysis of HeLa cell using PEBP1 antibody. Blue: DAPI for nuclear staining.)
Western Blot (WB) (Western blot analysis of SERPINA1 Antibody (Center) in A2058 cell line lysates (35ug/lane). SERPINA1 (arrow) was detected using the purified Pab.)
Western Blot (WB) (Western blot analysis of ARHE Antibody (Center) in mouse brain tissue lysates (35ug/lane). ARHE (arrow) was detected using the purified Pab.)
Immunohistochemistry (IHC) (Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with ARHE antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Testing Data (Confocal fluorescent analysis of NIH/3T3 cells using MBS605927 (Red) (1:500). Actin filaments have been labeled with AlexaFluor®488 -Phalloidin (Green). Nucleus is stained with DAPI (Blue). This antibody positively stains the cytoplasm.)
Testing Data (HEK293 cell lysate was resolved by electrophoresis, transferred to PVDF membranes and probed with MBS605927 (1ug/ml). Proteins were visualized using a goat anti-mouse IgG conjugated to HRP and chemiluminescence detection system. Arrow indicates Raptor (~150kD).)
Dot Blot (DB) (Dot blot analysis of anti-p27Kip1-T157 Phospho-specific Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.6ug per ml.)
Immunohistochemistry (IHC) (Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
Western Blot (WB) (WFDC12 Antibody (C-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the WFDC12 antibody detected the WFDC12 protein (arrow).)
Immunohistochemistry (IHC) (WFDC12 antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the WFDC12 antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS) (WFDC12 Antibody (C-term) flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Western Blot (WB) (Western Blot analysis in Jurkat cell line lysates (35ug) usingMBS6001177. This demonstrates that MBS6001177 detected the CDKN1A protein (arrow).)
Western Blot (WB) (Western Blot analysis in mouse Neuro-2a cell line lysates (35ug) usingMBS6001177. This demonstrates that MBS6001177 detected the CDKN1A protein (arrow).)
Immunohistochemistry (IHC) (Immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma using MBS6001177 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS6001177 for immunohistochemistry.)
Western Blot (WB) (Western blot analysis of p19 Antibody (N-term) in mouse testis tissue lysates (35ug/lane). p19 (arrow) was detected using the purified Pab.)
Immunohistochemistry (IHC) (p19 Antibody (N-term) IHC analysis in formalin fixed and paraffin embedded testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the p19 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS) (p19 Antibody (N-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Western Blot (WB) (Western Blot analysis of Y79 cell lysate probed with 1ug/ml of P3140.)
Western Blot (WB) (Western blot analysis of SERPINA9 Antibody (Center) in MCF-7 cell line lysates (35ug/lane). SERPINA9 (arrow) was detected using the purified Pab.)
Immunohistochemistry (IHC) (Formalin-fixed and paraffin-embedded human lymph with SERPINA9 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS) (Flow cytometric analysis of MDA-231 cells using SERPINA9 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Western Blot (WB) (SERPINI1 monoclonal antibody (M03), clone 1E10. Western Blot analysis of SERPINI1 expression in HeLa.)
Testing Data (Detection limit for recombinant GST tagged SERPINI1 is 3 ng/ml as a capture antibody.)
Western Blot (WB) (Western blot analysis of extracts of NIH3T3 cells, using CDKN1B antibody.)
Testing Data (Detection limit for recombinant GST tagged SERPINA3 is 0.3 ng/ml as a capture antibody.)
Western Blot (WB) (Western Blot analysis of SERPINB2 expression in transfected 293T cell line by SERPINB2 polyclonal antibody. Lane 1: SERPINB2 transfected lysate (46.6kD). Lane 2: Non-transfected lysate.)
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