$480/0.1 mL (Biotin) | $480/0.1 mL (AF350) | $480/0.1 mL (AF405) | $480/0.1 mL (AF488) | $480/0.1 mL (AF555) | $480/0.1 mL (AF594) | $480/0.1 mL (AF647) | $480/0.1 mL (AF680) | $480/0.1 mL (AF750)
$480/0.1 mL (Biotin) | $480/0.1 mL (AF350) | $480/0.1 mL (AF405) | $480/0.1 mL (AF488) | $480/0.1 mL (AF555) | $480/0.1 mL (AF594) | $480/0.1 mL (AF647) | $480/0.1 mL (AF680) | $480/0.1 mL (AF750)
Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were Purified by affinity-chromatography using epitope-specific peptide.
$310/0.1 mL | $570/0.1 mL (Biotin) | $1,330/5x0.1 mL | $570/0.1 mL (FITC) | $570/0.1 mL (AF350) | $570/0.1 mL (AF405) | $570/0.1 mL (AF488) | $570/0.1 mL (AF555) | $570/0.1 mL (AF568)
Western Blot (WB) (Western Blot analysis of FGFR4 recombinant protein (fragment 42kD) usingMBS609945. FGFR4 protein (arrow) was detected using the purified Mab.)
Western Blot (WB) (Western Blot analysis in 293 cell lysates (2ug/lane) using MBS609945 either nontransfected (Lane 1) or transiently transfected with the FGFR4 gene (Lane 2).)
Western Blot (WB) (Western blot analysis of extracts of various cell lines, using FGFR2 antibody.)
Immunofluorescence (IF) (Immunofluorescence analysis of MCF-7 cell using FGFR2 antibody. Blue: DAPI for nuclear staining.)
Western Blot (WB) (Western Blot Positive WB detected in: Rat Brain whole cell lysate, Rat Lung whole cell lysate, Mouse Brain whole cell lysate All lanes: FGFR2 Antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 93, 87, 93, 77, 92, 89, 86, 29, 80, 93, 97, 92, 41, 80, 80, 77, 80 kDa Observed band size: 145 kDa)
Western Blot (WB) (Western Blot analysis using MBS630317 at 1:1000 dilution Lane 1: NCI-H460 whole cell lysate Lane 2: HT-29 whole cell lysate Lane 3: A549 whole cell lysate Lane 4: T47D whole cell lysate Lane 5: Hela whole cell lysate Lane 6: NIH/3T3 whole cell lysate Lane 7: Mouse brain lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 92 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB) (Western blot analysis of anti-FGFR2 Antibody (N-term R22) in Hela cell line lysates (35ug/lane). FGFR2(arrow) was detected using the purified Pab.)
Immunofluorescence (IF) (Confocal immunofluorescent analysis of FGFR2 Antibody (N-term R22) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).)
Flow Cytometry (FC/FACS) (FGFR2 Antibody (N-term R22) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Western Blot (WB) (Western Blot analysis using MBS630317 at 1:1000 dilution Lane 1: NCI-H460 whole cell lysate Lane 2: HT-29 whole cell lysate Lane 3: A549 whole cell lysate Lane 4: T47D whole cell lysate Lane 5: Hela whole cell lysate Lane 6: NIH/3T3 whole cell lysate Lane 7: Mouse brain lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 92 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB) (Western Blot analysis using MBS630317 at 1:1000 dilution Lane 1: NCI-H460 whole cell lysate Lane 2: HT-29 whole cell lysate Lane 3: A549 whole cell lysate Lane 4: T47D whole cell lysate Lane 5: Hela whole cell lysate Lane 6: NIH/3T3 whole cell lysate Lane 7: Mouse brain lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 92 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB) (Western blot analysis of anti-FGFR2 Antibody (N-term R22) in Hela cell line lysates (35ug/lane). FGFR2(arrow) was detected using the purified Pab.)
Immunofluorescence (IF) (Confocal immunofluorescent analysis of FGFR2 Antibody (N-term R22) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).)
Flow Cytometry (FC/FACS) (FGFR2 Antibody (N-term R22) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Western Blot (WB) (Western blot analysis of extracts from various samples, using FGFR1/2 Antibody.Lane 1: MCF7 cells(UV treatment), blocked with antigen-specific peptides,Lane 2: MCF7 cells(UV treatment),Lane 3: Hela cells(LPS treatment),Lane 4: Hela cells(heat shock treatment).)
Western Blot (WB) (Western Blot analysis of JK cells using Phospho-FGFR-4 (Y642) Polyclonal Antibody)
Western Blot (WB) (Western blot analysis of FGFR4 (pY642) expression in HepG2 (A), SGC7901 (B), Panc1 (C) whole cell lysates.)
Western Blot (WB) (Western blot analysis of FGFR4 (arrow) using rabbit polyclonal FGFR4 Antibody.293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the FGFR4 gene (Lane 2) (Origene Technologies).)
Western Blot (WB) (Western blot analysis of FGFR4 (arrow) using rabbit polyclonal FGFR4 Antibody.293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the FGFR4 gene (Lane 2) (Origene Technologies).)
Western Blot (WB) (Western blot analysis of extract from Rat liver and Rat brain tissue using FGFR4 Antibody )
Western Blot (WB) (Western Blot analysis of FGFR4 recombinant protein (fragment 42kD) using. FGFR4 protein (arrow) was detected using the purified Mab.)
Western Blot (WB) (Western Blot analysis in 293 cell lysates (2ug/lane) using either nontransfected (Lane 1) or transiently transfected with the FGFR4 gene (Lane 2).)
Western Blot (WB) (Western Blot analysis of FGFR4 recombinant protein (fragment 42kD) using. FGFR4 protein (arrow) was detected using the purified Mab.)
Western Blot (WB) (Western Blot analysis in 293 cell lysates (2ug/lane) using either nontransfected (Lane 1) or transiently transfected with the FGFR4 gene (Lane 2).)
Western Blot (WB) (Western Blot detection against Immunogen (38.39kD).)
Testing Data (Detection limit for recombinant GST tagged FGFR4 is 0.3ng/ml as a capture antibody.)
Testing Data (Proximity Ligation Analysis (PLA) of protein-protein interactions between FGF1 and FGFR4 HeLa cells were stained with FGF1 rabbit purified polyclonal 1:1200 and FGFR4 mouse monoclonal antibody 1:50. Signals were detected by 30 Detection Kit 613 (red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.)
Western Blot (WB) (Western Blot analysis using MBS630317 at 1:1000 dilution Lane 1: NCI-H460 whole cell lysate Lane 2: HT-29 whole cell lysate Lane 3: A549 whole cell lysate Lane 4: T47D whole cell lysate Lane 5: Hela whole cell lysate Lane 6: NIH/3T3 whole cell lysate Lane 7: Mouse brain lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 92 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB) (Western Blot analysis using MBS630317 at 1:1000 dilution Lane 1: NCI-H460 whole cell lysate Lane 2: HT-29 whole cell lysate Lane 3: A549 whole cell lysate Lane 4: T47D whole cell lysate Lane 5: Hela whole cell lysate Lane 6: NIH/3T3 whole cell lysate Lane 7: Mouse brain lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 92 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB) (Western Blot analysis using MBS630317 at 1:1000 dilution Lane 1: NCI-H460 whole cell lysate Lane 2: HT-29 whole cell lysate Lane 3: A549 whole cell lysate Lane 4: T47D whole cell lysate Lane 5: Hela whole cell lysate Lane 6: NIH/3T3 whole cell lysate Lane 7: Mouse brain lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 92 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB) (Western Blot analysis using MBS630317 at 1:1000 dilution Lane 1: NCI-H460 whole cell lysate Lane 2: HT-29 whole cell lysate Lane 3: A549 whole cell lysate Lane 4: T47D whole cell lysate Lane 5: Hela whole cell lysate Lane 6: NIH/3T3 whole cell lysate Lane 7: Mouse brain lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 92 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
Immunofluorescence (IF) (Fluorescent confocal image of HeLa cells stained withMBS6012430. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with MBS6012430 (1:200, 2 hours at RT). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1 hour). Nuclei were counterstained with Hoechst 33342 (blue) (10ug/ml, 5 min). Note the highly specific localization of the FGFR4 mainly to the nucleus, supported by Human Protein Atlas Data)
Western Blot (WB) (Western Bblot analysis of MBS6012430 in 293 cell line lysates (35ug/lane).This demonstrates the FGFR4 antibody detected the FGFR4 protein (arrow).)
Flow Cytometry (FC/FACS) (Flow Cytometric analysis of WiDr cells using MBS6012430 (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Immunohistochemistry (IHC) (Formalin-fixed and paraffin-embedded human cancer tissue reacted with theMBS6012430, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.)
Western Blot (WB) (Western blot analysis of extracts from various samples, using N Cadherin Antibody.Lane 1: Rat heart, blocked with antigen-specific peptides,Lane 2: Rat heart,Lane 3: P19 cells(serum starvation treatment),Lane 4: HepG2 cells(heat shock treatment).)
Immunohistochemistry (IHC) (Staining Mouse testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
Immunohistochemistry (IHC) (At 1/100 staining Mouse liver tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
Immunohistochemistry (IHC) (At 1/100 staining Human colorectal cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
Western Blot (WB) (Western blot analysis of 293T 3T3 lysis using FGFR4 antibody. Antibody was diluted at 1:500. Secondary antibody was diluted at 1:20000)
Western Blot (WB) (Western blot analysis of FGFR4 (arrow) using rabbit polyclonal FGFR4 Antibody.293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the FGFR4 gene (Lane 2) (Origene Technologies).)
Western Blot (WB) (Western Blot detection against Immunogen (38.39kD).)
Testing Data (Detection limit for recombinant GST tagged FGFR4 is 0.3ng/ml as a capture antibody.)
Testing Data (Proximity Ligation Analysis (PLA) of protein-protein interactions between FGF1 and FGFR4 HeLa cells were stained with FGF1 rabbit purified polyclonal 1:1200 and FGFR4 mouse monoclonal antibody 1:50. Signals were detected by 30 Detection Kit 613 (red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.)
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