$310/0.1 mg | $1,330/5x0.1 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
The antibody was purified by immunogen affinity chromatography.
Pricing
$310/0.1 mL | $465/0.1 mL (Biotin) | $1,330/5x0.1 mL | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
The antibody was purified by immunogen affinity chromatography.
Pricing
$310/0.1 mL | $465/0.1 mL (Biotin) | $1,330/5x0.1 mL | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
Western Blot (WB) (Western blot detection of phospho-Met (c-Met) (Tyr1349) in Hela,A549 cell lysates using phospho-Met (c-Met) (Tyr1349) Rabbit mAb(1:1000 diluted).Predicted band size:156kDa.Observed band size:170-140kDa.)
Western Blot (WB) (Anti-C-met rabbit polyclonal antibody at 1:500 dilutionLane A: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:155 kDaObserved band size:140 kDa(We are unsure as to the identity of these extra bands.))
Immunohistochemistry (IHC) (IHC image diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.)
Immunohistochemistry (IHC) (IHC image diluted at 1:100 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.)
Immunohistochemistry (IHC) (Immunohistochemical staining of paraffin-embedded MET positive Human lung carcinoma tissue using anti-MET mouse monoclonal antibody. (TA805796) Dilution: 1:150)
Wester Blot (WB) (Dilution: Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.)
Wester Blot (WB) (Dilution: Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.)
Western Blot (WB) (Western blot analysis of extracts of various cell lines, using MET antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 10s.)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human lung cancer using MET Rabbit pAb at dilution of 1:100 (40x lens).)
SDS-Page (SDS PAGE for Onartuzumab)
SDS-Page (SDS PAGE for Telisotuzumab)
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