Search results for "cytoplasmic-aconitate-hydratase"

FCM (Flow Cytometry) (Figure 7. Flow Cytometry analysis of SiHa cells using anti-Aconitase 1/ACO1 antibody (AAA19267).Overlay histogram showing SiHa cells stained with AAA19267 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Aconitase 1/ACO1 Antibody (AAA19267,1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 6. IF analysis of Aconitase 1/ACO1 using anti-Aconitase 1/ACO1 antibody (AAA19267).Aconitase 1/ACO1 was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 4μg/mL rabbit anti-Aconitase 1/ACO1 Antibody (AAA19267) overnight at 4 degree C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

IHC (Immunohistochemistry) (Figure 5. IHC analysis of Aconitase 1/ACO1 using anti-Aconitase 1/ACO1 antibody (AAA19267).Aconitase 1/ACO1 was detected in paraffin-embedded section of rat intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Aconitase 1/ACO1 Antibody (AAA19267) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 4. IHC analysis of Aconitase 1/ACO1 using anti-Aconitase 1/ACO1 antibody (AAA19267).Aconitase 1/ACO1 was detected in paraffin-embedded section of human renal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Aconitase 1/ACO1 Antibody (AAA19267) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 3. IHC analysis of Aconitase 1/ACO1 using anti-Aconitase 1/ACO1 antibody (AAA19267).Aconitase 1/ACO1 was detected in paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Aconitase 1/ACO1 Antibody (AAA19267) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 2. IHC analysis of Aconitase 1/ACO1 using anti-Aconitase 1/ACO1 antibody (AAA19267).Aconitase 1/ACO1 was detected in paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Aconitase 1/ACO1 Antibody (AAA19267) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

WB (Western Blot) (Figure 1. Western blot analysis of Aconitase 1/ACO1 using anti-Aconitase 1/ACO1 antibody (AAA19267).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human HEK293 whole cell lysatesLane 2: human HepG2 whole cell lysatesLane 3: human U20S wholel cell lysatesLane 4: monkey liver tissue lysatesLane 5: rat liver tissue lysatesLane 6: rat kidney tissue lysatesLane 7: mouse liver tissue lysatesLane 8: mouse kidney tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-Aconitase 1/ACO1 antigen affinity purified polyclonal antibody (Catalog # AAA19267) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # with Tanon 5200 system. A specific band was detected for Aconitase 1/ACO1 at approximately 98KD. The expected band size for Aconitase 1/ACO1 is at 98KD.)

Loading...

Filters

Clonality

Type

Reactivity

Gene Name

Isotype

Host

Application

Clone

1 results for "cytoplasmic-aconitate-hydratase" - showing 1-1

Aconitase 1/ACO1, Polyclonal Antibody (Cat# AAA19267)

Contact us by