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Gene Name
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Application
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2162 results for "Viral Proteins" - showing 1-50
Western Blot (WB)
(Western blot analysis of extracts of 293 and HeLa cells, using Phospho-AKT1-T450 antibody at 1:1000 dilution. 293 cells were treated by Insulin (100nM) for 10 minutes after serum-starvation overnight. HeLa cells were treated by EGF (100ng/ml) for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit.Exposure time: 1min.)
AKT1-T450, Antibody (Cat# AAA125441)
Full Name
Phospho-AKT1-T450 Polyclonal Antibody
Gene Names
AKT1; AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF
Purity
Affinity Purification
Pricing
$260/0.05 mL | $385/0.1 mL | $570/0.2 mL
Western Blot (WB)
(Western blot analysis of extracts from NIH/3T3 using Phospho-AKT1-S473 antibody.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.)
AKT1-S473, Antibody (Cat# AAA128348)
Full Name
Phospho-AKT1-S473 Polyclonal Antibody
Gene Names
AKT1; AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA
Reactivity
Human, Mouse, Rat
Applications
WB, IHC
Purity
Affinity Purification
Pricing
$180/0.02 mL | $250/0.05 mL | $350/0.1 mL | $525/0.2 mL | $2,210/5x0.2 mL
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using AKT1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.)
AKT1, Polyclonal Antibody (Cat# AAA9125011)
Full Name
AKT1 Polyclonal Antibody
Gene Names
AKT1; AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA
Reactivity
Human, Mouse, Rat
Applications
WB, IHC
Purity
Affinity Purification
Pricing
$180/0.02 mL | $210/0.05 mL | $285/0.1 mL | $430/0.2 mL | $1,790/5x0.2 mL
Western Blot (WB)
(Western blot analysis of extracts of HeLa cells, using Phospho-AKT1-T308 antibody at 1:2000 dilution. HeLa cells were treated by 10% FBS for after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Enhanced Kit.Exposure time: 90s.)
AKT1-T308, Antibody (Cat# AAA128342)
Full Name
Phospho-AKT1-T308 Polyclonal Antibody
Gene Names
AKT1; AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA
Reactivity
Human, Mouse
Applications
WB, IHC, IF
Purity
Affinity Purification
Pricing
$180/0.02 mL | $250/0.05 mL | $350/0.1 mL | $525/0.2 mL | $2,210/5x0.2 mL
Testing Data
(SDS-PAGE: Purified HIV-1 gp120 (JRFL) (Clade B) protein)
gp120 (JRFL) (HIV-1/Clade B), Recombinant Protein (Cat# AAA434050)
Full Name
gp120 (JRFL) (HIV-1/Clade B) (aa 34-518)
Applications
WB, EIA
Purity
≥ 95% purity (SDS-PAGE)
Pricing
$370/0.05 mg | $1,525/5x0.05 mg
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using AKT1 antibody.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 30s.)
AKT1, Polyclonal Antibody (Cat# AAA9127855)
Full Name
AKT1 Polyclonal Antibody
Gene Names
AKT1; AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF
Purity
Affinity Purification
Pricing
$225/0.05 mL | $305/0.1 mL | $465/0.2 mL
Immunofluorescence (IF)
(Firure 1. Immunofluorescent Validation of MBS150780 in SARS-CoV-2 Infected Lung Tissue (Singh et al., Nature Microbiology, 2021)Multilabel confocal immunofluorescence microscopy of formalin-fixed paraffin-embedded lung sections from rhesus macaques infected with SARS-CoV-2. SARS-CoV-2 spike-specific antibodies, MBS150780. (k, n) (turquoise); Ki67 (magenta) and neutrophil marker CD66abce (yellow) (k); pan-macrophage marker CD68 (magenta) (n) and DAPI (blue).)
COVID 19 Spike Protein Coronavirus, Polyclonal Antibody (Cat# AAA150780)
Full Name
SARS-CoV-2 (COVID-19, 2019-nCoV) Spike Antibody
Reactivity
Virus
Applications
IF, IHC, WB
Purity
SARS-CoV-2 (COVID-19, 2019-nCoV) Spike Antibody is affinity chromatography purified via peptide column.
Pricing
$440/0.1 mg | $1,960/5x0.1 mg
Toxoplasma Gondii P30, Recombinant Protein (Cat# AAA143160)
Full Name
Recombinant Toxoplasma Gondii P30 (SAG 1)
Applications
EIA, WB
Purity
Purity: Protein is >95% pure as determined by 10% SDS-PAGE (Coomasie staining)
Purification Method: Purified by proprietary chromatographic technique.
Purification Method: Purified by proprietary chromatographic technique.
Pricing
$270/0.1 mg | $710/0.5 mg | $1,110/1 mg | $4,780/5x1 mg
Testing Data
(SDS-PAGE(12%): purified E2 (HCV)(aa 383-663), subtype 1b)
E2 (HCV), Recombinant Protein (Cat# AAA434003)
Full Name
E2 (HCV) (amino acid 383-663), subtype 1b
Applications
Western Blot standard, antibody ELISA, antigen, etc.
Purity
> = 96% purity
Pricing
$355/0.05 mg | $1,440/5x0.05 mg
Western Blot (WB)
(Western blot analysis of extracts from HepG2 cells untreated or treated with EGF using Phospho-AKT1-Y315/AKT2-Y316/AKT3-Y312 antibody.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.)
AKT1/2/3-Y315/316/312, Antibody (Cat# AAA128418)
Full Name
Phospho-AKT1/2/3-Y315/316/312 Polyclonal Antibody
Gene Names
AKT1; AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF
Purity
Affinity Purification
Pricing
$260/0.05 mL | $385/0.1 mL | $570/0.2 mL
SDS-PAGE (SDS)
(SDS-PAGE: Purified S1 protein (aa 1-725) of MERS-CoV)
MERS CoV Spike S1, Recombinant Protein (Cat# AAA434229)
Full Name
Spike Protein (S1)(aa 1-725) of MERS CoV
Applications
Western blot standard, antibody ELISA, antigen, etc.
Purity
> 95% (by SDS PAGE)
Pricing
$385/0.05 mg | $1,580/5x0.05 mg
Coronavirus 229E, Recombinant Protein (Cat# AAA145566)
Full Name
Recombinant Coronavirus 229E
Purity
Protein is >95% pure as determined by 12% PAGE (coomassie staining).
Pricing
$270/0.1 mg | $710/0.5 mg | $1,315/1 mg | $5,695/5x1 mg
Malaria Protein HSP, Recombinant Protein (Cat# AAA142737)
Full Name
Recombinant Malaria Protein HSP
Applications
EIA, WB
Purity
Protein is >95% pure as determined by 10% PAGE (coomassie staining) and RP-HPLC.
Purified by proprietary chromatographic technique.
Purified by proprietary chromatographic technique.
Pricing
$270/0.1 mg | $710/0.5 mg | $1,315/1 mg | $5,695/5x1 mg
Testing Data
(SDS - PAGE : purified HA (H5N1) (A/Vietnam/1203/2004) (aa18 - 530) protein.)
H5 (H5N1) (A/Vietnam/1203/2004), Recombinant Protein (Cat# AAA434010)
Full Name
H5 (H5N1) (aa 18-530)(A/Vietnam/1203/2004)
Applications
Western blot standard, antibody ELISA, antigen, etc.
Purity
≥ 95% (SDS-PAGE)
Pricing
$355/0.05 mg | $1,440/5x0.05 mg
SDS-PAGE
(SDS-PAGE: purified HA1 (H1N1)(A//Puerto Rico/8/1934) protein)
HA1 (H1N1)(A/PR/8/34), Recombinant Protein (Cat# AAA434017)
Full Name
HA1 (H1N1) (aa 18-345)(A/PR/8/34)
Applications
Western blot standard, antibody ELISA, antigen, etc
Purity
> 95% by SDS PAGE
Pricing
$450/0.05 mg | $1,880/5x0.05 mg
Western Blot (WB)
(The anti-ACE2 C-term Pab (MBS9212149) is used in Western blot to detect ACE2 in 293 cell lysate.)
ACE2 (SARS Receptor), Polyclonal Antibody (Cat# AAA9212149)
Full Name
ACE2 (SARS Receptor) Antibody (C-term)
Gene Names
ACE2; ACEH
Reactivity
Human
Applications
WB, EIA, IHC-P
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Testing Data
HA1 (H3N2)(A/Wyoming/3/03), Recombinant Protein (Cat# AAA434021)
Full Name
HA1 (H3N2) (aa 18-347)(A/Wyoming/3/03)
Applications
WB, EIA
Purity
> 95% (SDS-PAGE)
Pricing
$425/0.05 mg | $1,760/5x0.05 mg
SARS Associated Coronavirus Matrix, Recombinant Protein (Cat# AAA143135)
Full Name
Recombinant SARS Associated Coronavirus Matrix
Applications
EIA, WB
Purity
SARS Matrix Protein is >95% pure as determined by 10% PAGE (coomassie staining).
SARS Matrix protein was purified by proprietary chromatographic technique.
SARS Matrix protein was purified by proprietary chromatographic technique.
Pricing
$270/0.1 mg | $710/0.5 mg | $1,110/1 mg | $4,780/5x1 mg
Western Blot (WB)
(Anti-KRAS Antibody (C-term) at 1:2000 dilution + HT-29 whole cell lysate Lysates/proteins at 20 ug per lane.Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 22 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
KRAS, Polyclonal Antibody (Cat# AAA9210256)
Full Name
KRAS Antibody (C-term)
Gene Names
KRAS; NS; NS3; CFC2; KRAS1; KRAS2; RASK2; KI-RAS; C-K-RAS; K-RAS2A; K-RAS2B; K-RAS4A; K-RAS4B
Reactivity
Human, Mouse, Rat
Predicted: Rat
Predicted: Rat
Applications
WB, EIA, IF, FC/FACS
Pricing
$185/0.05 mL | $405/0.2 mL | $1,585/5x0.2 mL
Western Blot (WB)
(L:Western blot detection of DSBA in AKT1,AKT2,AKT3 and DSBA recombinant antigen fragments the same sample quality, and using DSBA antibody (1:1000 diluted).R:Western blot detection of AKT in AKT1,AKT2 and AKT3 recombinant antigen fragments and using AKT antibody (1:1000 diluted).)
total AKT, Monoclonal Antibody (Cat# AAA9400291)
Full Name
total AKT Monoclonal Antibody
Gene Names
AKT1; AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA
Reactivity
Human, Mouse, Rat
Applications
WB, IP
Purity
Affinity purified
Pricing
$305/0.1 mL | $1,230/5x0.1 mL
Testing Data
(Published customer image: Mouse anti V5 tag antibody, clone SV5-Pk1 used for detection of V5 tagged REDD1 protein by western blotting.Image caption:REDD1 is not degraded by Cul4a or phosphorylated by GSK3beta at Thr 23 and Thr25. (A) HEK293 cells were transfected with empty vector or tetracycline inducible dnCul4a-V5 pcDNA4/TO (1 ug) and Cul4A-V5 pcDNA3 (0.03 ug) followed by tetracycline (1 ug/ml) induction for 24 hours and cell lysis. (B) HEK293 cells were transfected with 0.5 ug Cul4a-V5 pcDNA3 for 15 hours followed by transfection of 20 nM control or Cul4a siRNAs to determine siRNAs efficiency. (C) HEK293 cells were transfected with 20 nM control or Cul4a siRNAs for 3 days followed by cell lysis. (D) REDD1-V5 pcDNA3 wild type, T23A T25A or T23D T25D plasmids (0.4 ug) were transfected in HEK293 cells for 3 days and treated with 20 uM MG-132 for 6 hours followed by cell lysis. (E) HEK293 cells were treated with 30 mM LiCl or GSK3 inhibitor IX (5 uM or 10 uM) for 20 hours followed by cell lysis. (F) HEK293 cells were co-transfected with 0.2 ug REDD1-V5 pcDNA3 and 0.3 ug GSK3beta pcDNA3 or empty pcDNA3 for 3 days followed by MG-132 (20 uM) treatment for 6 hours followed by cell lysis. (G) HEK293 cells were transfected with 3 ug DYKDDDDK-REDD1 or DYKDDDDK-FRAT1 for 3 days followed by cell lysis and DYKDDDDK immunoprecipitation. In vitro phosphorylation of REDD1 and FRAT1 was carried out as described in Materials and Methods..From: Tan CY, Hagen T (2013) mTORC1 Dependent Regulation of REDD1 Protein Stability. PLoS ONE 8(5): e63970.)
V5-TAG, Monoclonal Antibody (Cat# AAA212109)
Full Name
MOUSE ANTI V5-TAG
Applications
EIA, FC/FACS, IF, IP, WB, RIA
Pricing
$785/1 mg | $3,370/5x1 mg
SDS-PAGE (SDS)
(SDS-PAGE: purified HIV-1 gp120 (HXBc2) (Clade B) protein)
gp120 (HXBc2) (HIV-1/Clade B), Recombinant Protein (Cat# AAA434048)
Full Name
gp120 (HXBc2) (HIV-1/Clade B) (aa 34-518)
Applications
WB, EIA
Purity
> 95% purity (SDS PAGE).
Pricing
$370/0.05 mg | $1,525/5x0.05 mg
Respiratory Syncytial Virus (RSV) Fusion Protein, Monoclonal Antibody (Cat# AAA310162)
Full Name
MAb to RSV Fusion Protein
Applications
EIA/ELISA, Immunofluorescence Assay, Neut, Immunoassay Antibody Pairs
Purity
>90% pure. Protein A chromatography
Pricing
$1,025/1 mg | $4,460/5x1 mg
SDS-PAGE
( )
Ebola Virus NP, Recombinant Protein (Cat# AAA5304199)
Full Name
Ebola Virus NP protein
Applications
WB
Purity
> 90% pure
Purified by chromatograpy from Zaire stain, this nucleoprotein is phosphorylated and O-glycosolated by the host organisms cells.
Purified by chromatograpy from Zaire stain, this nucleoprotein is phosphorylated and O-glycosolated by the host organisms cells.
Pricing
$230/0.1 mg | $1,020/5x0.1 mg
Human Immunodeficiency Virus Type-1 p24, Monoclonal Antibody (Cat# AAA438224)
Full Name
Human Immunodeficiency Virus Type-1 p24
Reactivity
HIV Type 1. Others not known.
Applications
EIA, ICC, IF
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA at 1mg/mL)
SARS Coronavirus Nucleocapsid, Recombinant Protein (Cat# AAA143134)
Full Name
Recombinant SARS Coronavirus Nucleocapsid (1-422)
Purity
Greater than 95% as determined by SDS-PAGE.
Pricing
$250/0.02 mg | $4,415/1 mg | $180/0.005 mg | $19,645/5x1 mg
Western Blot (WB)
(Western blot detection of EGFR in A549, MDA-MB-468 and COS7 cell lysates using EGFR mouse mAb (dilution 1:1000).Predicted band size:134 Kda.Observed band size:175KDa.)
EGF Receptor, Monoclonal Antibody (Cat# AAA475155)
Full Name
Anti-EGF Receptor Mouse mAb
Gene Names
EGFR; ERBB; HER1; mENA; ERBB1; PIG61; NISBD2
Reactivity
Human, Monkey
Applications
WB, ICC, IP
Purity
Affinity Purified
Pricing
$440/0.1 mL | $1,530/5x0.1 mL
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using MYCN antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)
MYCN, Polyclonal Antibody (Cat# AAA125752)
Full Name
MYCN Polyclonal Antibody
Gene Names
MYCN; NMYC; ODED; MODED; N-myc; bHLHe37
Reactivity
Human, Mouse, Rat
Applications
WB, IHC
Purity
Affinity Purification
Pricing
$180/0.02 mL | $210/0.05 mL | $285/0.1 mL | $430/0.2 mL | $1,790/5x0.2 mL
Western Blot (WB)
(Western blot detection of EGFR in A549 and COS7 cell lysates using EGFR mouse mAb (dilution 1:2000).Predicted band size:134 Kda.Observed band size:175KDa.)
EGF Receptor, Monoclonal Antibody (Cat# AAA475211)
Full Name
Anti-EGF Receptor Mouse mAb
Gene Names
EGFR; ERBB; HER1; mENA; ERBB1; PIG61; NISBD2
Reactivity
Human, Monkey
Applications
WB, ICC, IP
Purity
Affinity Purified
Pricing
$440/0.1 mL | $1,530/5x0.1 mL
Testing Data
(Published customer image: Mouse anti V5 tag antibody, clone SV5-Pk1 used for detection of V5 tagged REDD1 protein by western blotting.Image caption:REDD1 is not degraded by Cul4a or phosphorylated by GSK3beta at Thr 23 and Thr25. (A) HEK293 cells were transfected with empty vector or tetracycline inducible dnCul4a-V5 pcDNA4/TO (1 ug) and Cul4A-V5 pcDNA3 (0.03 ug) followed by tetracycline (1 ug/ml) induction for 24 hours and cell lysis. (B) HEK293 cells were transfected with 0.5 ug Cul4a-V5 pcDNA3 for 15 hours followed by transfection of 20 nM control or Cul4a siRNAs to determine siRNAs efficiency. (C) HEK293 cells were transfected with 20 nM control or Cul4a siRNAs for 3 days followed by cell lysis. (D) REDD1-V5 pcDNA3 wild type, T23A T25A or T23D T25D plasmids (0.4 ug) were transfected in HEK293 cells for 3 days and treated with 20 uM MG-132 for 6 hours followed by cell lysis. (E) HEK293 cells were treated with 30 mM LiCl or GSK3 inhibitor IX (5 uM or 10 uM) for 20 hours followed by cell lysis. (F) HEK293 cells were co-transfected with 0.2 ug REDD1-V5 pcDNA3 and 0.3 ug GSK3beta pcDNA3 or empty pcDNA3 for 3 days followed by MG-132 (20 uM) treatment for 6 hours followed by cell lysis. (G) HEK293 cells were transfected with 3 ug DYKDDDDK-REDD1 or DYKDDDDK-FRAT1 for 3 days followed by cell lysis and DYKDDDDK immunoprecipitation. In vitro phosphorylation of REDD1 and FRAT1 was carried out as described in Materials and Methods..From: Tan CY, Hagen T (2013) mTORC1 Dependent Regulation of REDD1 Protein Stability. PLoS ONE 8(5): e63970.)
V5-TAG, Monoclonal Antibody (Cat# AAA210328)
Full Name
MOUSE ANTI V5-TAG:Biotin
Applications
EIA, WB
Pricing
$445/0.1 mg | $1,845/5x0.1 mg
Western Blot (WB)
(Western blot detection of c-Fos in HCT116, COS7 and C6 cell lysates using c-Fos mouse mAb (1:500 diluted).Predicted band size:62KDa.Observed band size:62KDa.)
c-Fos, Monoclonal Antibody (Cat# AAA475305)
Full Name
Anti-c-Fos Mouse mAb
Gene Names
FOS; p55; AP-1; C-FOS
Reactivity
Human, Rat, Monkey
Applications
WB
Purity
Affinity Purified
Pricing
$440/0.1 mL | $1,530/5x0.1 mL
Western Blot (WB)
(Western blot analysis of 20 ng of (A) viral p24 and (B) recombinant p24 with anti-HIV-1 p24 antibody PM-6585 at (A) 0.5 μg/mL and (B) 0.2 μg/mL, respectively.)
HIV-1 p24, Monoclonal Antibody (Cat# AAA153320)
Full Name
HIV-1 p24 Antibody [7F4]
Reactivity
Virus
Applications
EIA, WB
Purity
HIV-1 p24 antibody is supplied as protein A purified IgG1.
Pricing
$440/0.1 mg | $1,960/5x0.1 mg
Activity Data
(Golgi Protein 73 (GP73) also known as Golgi phosphoprotein 2 or Golgi membrane protein GP73 is a protein that encoded by the GOLM1 gene. The Golgi complex plays a key role in the sorting and modification of proteins exported from the endoplasmic reticulum. It has been observed to be upregulated in response to viral infection. Besides, Dymeclin (DYM) has been identified as an interactor of GP73, thus a binding ELISA assay was conducted to detect the interaction of recombinant mouse GP73 and recombinant human DYM. Briefly, GP73 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to DYM-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-GP73 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of of GP73 and DYM was shown in Figure 1, and this effect was in a dose dependent manner.)
Golgi Protein 73 (GP73), Active Protein (Cat# AAA2097297)
Full Name
Active Golgi Protein 73 (GP73)
Gene Names
Golm1; GP73; Golph2; AW125446; PSEC0257; 2310001L02Rik; D030064E01Rik
Reactivity
Mouse
Applications
Cell culture, Activity Assays
Purity
> 97%
Pricing
$265/0.01 mg | $595/0.05 mg | $920/0.1 mg | $1,135/0.2 mg | $2,240/0.5 mg | $3,315/1 mg | $8,120/5 mg
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using RELA antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.)
p65, Polyclonal Antibody (Cat# AAA127352)
Full Name
p65 Polyclonal Antibody
Gene Names
RELA; p65; NFKB3
Reactivity
Human, Rat
Applications
WB, IHC, IF
Purity
Affinity Purification
Pricing
$180/0.02 mL | $210/0.05 mL | $285/0.1 mL | $430/0.2 mL | $1,790/5x0.2 mL
V5-TAG, Monoclonal Antibody (Cat# AAA212110)
Full Name
MOUSE ANTI V5-TAG
Applications
FC/FACS, IF, IHC-F, IP, RIA, WB
Pricing
$255/0.1 mg | $995/5x0.1 mg
Western Blot (WB)
(Western blot detection of c-Fos antibody in HCT116,COS7 and C6 cell lysates using c-Fos antibody (1ïš500 diluted).Predicted band size:62KDa.Observed band size:62KDa.)
c-Fos, Monoclonal Antibody (Cat# AAA9400504)
Full Name
c-Fos Monoclonal Antibody
Gene Names
FOS; p55; AP-1; C-FOS
Reactivity
Human, Rat, Monkey
Applications
WB
Purity
Affinity purified
Pricing
$305/0.1 mL | $1,230/5x0.1 mL
Immunofluorescence (IF)
(Immunofluorescence staining of methanol-fixed Hela cells using NFkappaB-p100/p52 antibody)
NFKB p100, Polyclonal Antibody (Cat# AAA835763)
Full Name
NFKB p100 antibody
Gene Names
NFKB2; p52; p100; H2TF1; LYT10; CVID10; LYT-10; NF-kB2
Applications
IF, IHC, WB
Purity
NFKB p100 antibody was purified by immunoaffinity chromatography.
Pricing
$375/0.05 mg | $1,675/5x0.05 mg
Western Blot (WB)
(Western blot detection of PKR in Jurkat, A549 and MCF7 cell lysates using PKR mouse mAb (1:1000 diluted).Predicted band size:62KDa.Observed band size:74KDa.)
PKR, Monoclonal Antibody (Cat# AAA476586)
Full Name
Anti-PKR Mouse mAb
Gene Names
EIF2AK2; PKR; PRKR; EIF2AK1; PPP1R83
Reactivity
Human
Applications
WB
Pricing
$440/0.1 mL | $1,530/5x0.1 mL
Western Blot (WB)
(Western blot analysis of extracts of HeLa cells, using Phospho-RELA-S311 antibody at 1:2000 dilution. HeLa cells were treated by IFN-α (100ng/ml) for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Enhanced Kit.Exposure time: 90s.)
RELA-S311, Antibody (Cat# AAA128391)
Full Name
Phospho-RELA-S311 Polyclonal Antibody
Gene Names
RELA; p65; NFKB3
Reactivity
Human
Applications
WB, IHC, IF
Purity
Affinity Purification
Pricing
$180/0.02 mL | $250/0.05 mL | $350/0.1 mL | $525/0.2 mL | $2,210/5x0.2 mL
Activity Data
(The Interferon-alpha (IFNa) proteins belong to type I interferons (IFNs) which a large subgroup of interferon proteins that help regulate the activity of the immune system. The IFNa proteins produced by leukocytes are also known as leukocyte interferon. They are mainly involved in innate immune response against viral infection. Besides, Interferon-alpha 13 (IFNA13) has been identified as an interactor of IFNa, thus a binding ELISA assay was conducted to detect the interaction of recombinant mouse IFNa and recombinant mouse IFNA13. Briefly, IFNA were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to IFNA13-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-IFNapAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of IFNa and IFNA13 was shown in Figure 1, and this effect was in a dose dependent manner.)
Interferon Alpha (IFNa), Active Protein (Cat# AAA2097256)
Full Name
Active Interferon Alpha (IFNa)
Gene Names
Ifna1; Ifa1
Reactivity
Mouse
Applications
Cell culture, Activity Assays
Purity
> 97%
Pricing
$255/0.01 mg | $565/0.05 mg | $875/0.1 mg | $1,080/0.2 mg | $2,120/0.5 mg | $3,145/1 mg | $7,695/5 mg
Western Blot (WB)
(Western blot detection of B Raf in A549, Ramos, Jurkat, THP-1 and Raji cell lysates using B Raf mouse mAb (1:1000 diluted).Predicted band size:87KDa.Observed band size:87KDa.)
B raf, Monoclonal Antibody (Cat# AAA475896)
Full Name
Anti-B raf Mouse mAb
Gene Names
BRAF; NS7; B-raf; BRAF1; RAFB1; B-RAF1
Reactivity
Human
Applications
WB
Pricing
$440/0.1 mL | $1,520/5x0.1 mL
SARS Associated Coronavirus Envelope, Recombinant Protein (Cat# AAA143136)
Full Name
Recombinant SARS Associated Coronavirus Envelope
Applications
EIA, WB
Purity
Purified by propietary chromatographic technique.
Protein is >95% pure as determined by 10% PAGE (coomasie staining).
Protein is >95% pure as determined by 10% PAGE (coomasie staining).
Pricing
$270/0.1 mg | $710/0.5 mg | $1,110/1 mg | $4,780/5x1 mg
Western Blot (WB)
(Western blot analysis of extracts of HL-60 cells, using KIT antibody.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.)
Kit, Polyclonal Antibody (Cat# AAA9127879)
Full Name
KIT Polyclonal Antibody
Gene Names
KIT; PBT; SCFR; C-Kit; CD117
Reactivity
Human
Applications
WB, IHC
Purity
Affinity purification
Pricing
$180/0.02 mL | $210/0.05 mL | $285/0.1 mL | $430/0.2 mL | $1,790/5x0.2 mL
Western Blot (WB)
(Western blot analysis of Phospho-ABL-Y412 Antibody Pab in A2058 cell line lysate (35ug/lane). ABL (arrow) was detected using the purified Pab.)
Phospho-ABL (Y412), Polyclonal Antibody (Cat# AAA9204367)
Full Name
Phospho-ABL (Y412) Antibody
Gene Names
ABL1; ABL; JTK7; p150; c-ABL; v-abl; c-ABL1; bcr/abl
Reactivity
Human
(Predicted Reactivity: Zebrafish, Mouse)
(Predicted Reactivity: Zebrafish, Mouse)
Applications
WB, DB, EIA, IHC-P
Purity
This antibody is purified through a protein A column, followed by peptide affinity purification.
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Immunofluorescence (IF)
(MBS9600811 staining K562 cells by ICC/IF. Cells were fixed with PFA and permeabilized in 0.1% saponin prior to blocking in 10% serum for 45 minutes at 37 degree C. The primary antibody was diluted 1/400 and incubated with the sample for 1 hour at 37 degree C. A Alexa Fluor 594 conjugated goat polyclonal to rabbit IgG (H+L), diluted 1/600 was used as secondary antibody.)
Abl, Polyclonal Antibody (Cat# AAA9600811)
Full Name
Phospho-Abl (Tyr393/412) Antibody
Gene Names
ABL1; ABL; JTK7; p150; c-ABL; v-abl; CHDSKM; c-ABL1; bcr/abl
Reactivity
Human, Mouse, Monkey
Applications
WB, IHC, IF, ICC, EIA
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot
(Western blot analysis of lysate from SH-SY5Y cell line, using APG5L Antibody (D3). AP1812a was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.)
ATG5, Polyclonal Antibody (Cat# AAA9206419)
Full Name
ATG5 Antibody (N-term)
Gene Names
ATG5; ASP; APG5; APG5L; hAPG5; APG5-LIKE
Reactivity
Human;
(Predicted Reactivity: Zebrafish, Bovine, Mouse, Pig, Rat, Rabbit )
(Predicted Reactivity: Zebrafish, Bovine, Mouse, Pig, Rat, Rabbit )
Applications
WB, EIA, IHC-P, IF
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Testing Data
(Gel: 10%SDS-PAGELysate:2ug Recombinant HA tagged proteinPrimary antibody: 1/1000 dilutionSecondary antibody dilution: 1/8000Exposure time: 1 second)
c-Myc, Polyclonal Antibody (Cat# AAA9401449)
Full Name
c-Myc Tag Antibody
Gene Names
MYC; MRTL; MYCC; c-Myc; bHLHe39
Applications
WB
Purity
Antigen affinity purification.
Pricing
$305/0.1 mL | $1,230/5x0.1 mL
Testing Data
CD4 ectodomain, Recombinant Protein (Cat# AAA434073)
Full Name
Human CD4 ectodomain protein (amino acid 1-398)
Gene Names
CD4; CD4mut
Applications
WB, EIA
Purity
> 95% (SDS-PAGE)
Pricing
$410/0.05 mg | $1,700/5x0.05 mg
Testing Data
(Published customer image: Mouse anti V5 tag antibody, clone SV5-Pk1 used for detection of V5 tagged REDD1 protein by western blotting.Image caption:REDD1 is not degraded by Cul4a or phosphorylated by GSK3beta at Thr 23 and Thr25. (A) HEK293 cells were transfected with empty vector or tetracycline inducible dnCul4a-V5 pcDNA4/TO (1 ug) and Cul4A-V5 pcDNA3 (0.03 ug) followed by tetracycline (1 ug/ml) induction for 24 hours and cell lysis. (B) HEK293 cells were transfected with 0.5 ug Cul4a-V5 pcDNA3 for 15 hours followed by transfection of 20 nM control or Cul4a siRNAs to determine siRNAs efficiency. (C) HEK293 cells were transfected with 20 nM control or Cul4a siRNAs for 3 days followed by cell lysis. (D) REDD1-V5 pcDNA3 wild type, T23A T25A or T23D T25D plasmids (0.4 ug) were transfected in HEK293 cells for 3 days and treated with 20 uM MG-132 for 6 hours followed by cell lysis. (E) HEK293 cells were treated with 30 mM LiCl or GSK3 inhibitor IX (5 uM or 10 uM) for 20 hours followed by cell lysis. (F) HEK293 cells were co-transfected with 0.2 ug REDD1-V5 pcDNA3 and 0.3 ug GSK3beta pcDNA3 or empty pcDNA3 for 3 days followed by MG-132 (20 uM) treatment for 6 hours followed by cell lysis. (G) HEK293 cells were transfected with 3 ug DYKDDDDK-REDD1 or DYKDDDDK-FRAT1 for 3 days followed by cell lysis and DYKDDDDK immunoprecipitation. In vitro phosphorylation of REDD1 and FRAT1 was carried out as described in Materials and Methods..From: Tan CY, Hagen T (2013) mTORC1 Dependent Regulation of REDD1 Protein Stability. PLoS ONE 8(5): e63970.)
V5-TAG, Monoclonal Antibody (Cat# AAA214958)
Full Name
MOUSE ANTI V5-TAG:HRP
Applications
EIA, WB
Pricing
$490/0.1 mg | $2,060/5x0.1 mg
SARS Associated Coronavirus Nucleocaspid, Recombinant Protein (Cat# AAA143139)
Full Name
Recombinant SARS Associated Coronavirus Nucleocaspid (1-49,192-220)
Applications
EIA, WB
Purity
SARS Core protein is >95% pure as determined by 10% PAGE (coomassie staining).
SARS Core protein was purified by proprietary chromatographic technique.
SARS Core protein was purified by proprietary chromatographic technique.
Pricing
$270/0.1 mg | $710/0.5 mg | $1,110/1 mg | $4,780/5x1 mg
