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5 results for "Endocrine Metabolic Disease" - showing 1-5
Western Blot (WB)
(Western blot analysis of GAD67 expression in (1) Jurkat cell lysate; (2) C6 cell lysate (MBS179783).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GAD1 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for GAD1 )
GAD67, Monoclonal Antibody (Cat# AAA179783)
Full Name
Anti-GAD67 Rabbit Monoclonal Antibody
Gene Names
GAD1; GAD; SCP; CPSQ1
Reactivity
Human, Mouse, Rat
Applications
IP, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Chemical Structure
JD-5037, Inhibitor (Cat# AAA5750565)
Full Name
JD-5037
Purity
98.00%
Pricing
$205/2 mg | $235/5 mg | $295/10 mg | $385/25 mg | $260/1 mL (in DMSO) | $1,475/5x25 mg
Chemical Structure
3,5-Diiodo-L-thyronine, Inhibitor (Cat# AAA5750573)
Full Name
3,5-Diiodo-L-thyronine
Purity
98.00%
Pricing
$225/25 mg | $225/1 mL (in DMSO) | $755/5x25 mg
Western Blot (WB)
(Western blot analysis of extracts of 293T cells, using Phospho-IRS1-S323 antibody (MBS9144380) at 1:1000 dilution.293T cells were treated by IGF-1 (50 ng/mL) at 37 degree C for 5 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 10s.)
IRS1-S323, Polyclonal Antibody (Cat# AAA9144380)
Full Name
Phospho-IRS1-S323 Rabbit pAb
Gene Names
IRS1; HIRS-1
Reactivity
Human, Mouse, Rat
Applications
WB
Purity
Affinity purification
Pricing
$180/0.02 mL | $250/0.05 mL | $350/0.1 mL | $525/0.2 mL | $2,210/5x0.2 mL
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using ELOVL2 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 30s.)
ELOVL2, Polyclonal Antibody (Cat# AAA9142956)
Full Name
ELOVL2 Rabbit pAb
Gene Names
ELOVL2; SSC2
Reactivity
Human, Rat
Applications
WB
Purity
Affinity purification
Pricing
$180/0.02 mL | $210/0.05 mL | $285/0.1 mL | $430/0.2 mL | $1,790/5x0.2 mL
