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11 results for " ubiquitin ubiquitin like modifiers" - showing 1-11


Western Blot (WB) (Western blot using Rockland Immunochemicals affinity purified anti-Ube2j2 antibody shows detection of Ube2j2 in 293 cells over- expressing Myc-Ube2j2 (Lane 1). Lane 2 contains lysate from mock-transfected 293 cells.)

Ube2 J2, Polyclonal Antibody (Cat# AAA619701)

Full Name
Ube2 J2 (Ubiquitin-Conjugating Enzyme E2 J2)
Applications
EL/EIA, WB, IP
Purity
Affinity Purified
Purified by immunoaffinity chromatography.
Pricing
$855/0.1 mg | $3,845/5x0.1 mg
Western Blot (WB) (Western blot using Immunochemicals affinity purified anti-Ube2j1 antibody shows detection of Ube2j1 in 293 cells over- expressing Myc-Ube2j1 (Lane 2). Lane 1 contains lysate from mock- transfected 293 cells.)

Ube2 J1, Polyclonal Antibody (Cat# AAA622985)

Full Name
Ube2 J1 (Ubiquitin-Conjugating Enzyme E2 J1)
Applications
EL/EIA, WB, IP
Purity
Affinity Purified
Purified by immunoaffinity chromatography.
Pricing
$855/0.1 mg | $3,845/5x0.1 mg
Western Blot (WB) (Western blot analysis of SUMO2/3 in 15 ugs of HL60 cell lysate using IMG-571 at 1 ug/ml.)

Sentrin 2/3, Polyclonal Antibody (Cat# AAA617527)

Full Name
Sentrin 2/3 (SUMO 2/3)
Gene Names
SUMO2; HSMT3; SMT3B; SUMO3; Smt3A; SMT3H2
Reactivity
Human, Mouse
Applications
WB
Purity
Purified
Purified
Pricing
$560/0.1 mg | $2,505/5x0.1 mg
Western Blot (WB) (Western blot analysis of SENP2 in 15 ugs of Jurkat cell lysate using IMG-522 at 1:1000.)

SENP2, Polyclonal Antibody (Cat# AAA614534)

Full Name
SENP2, CT (Sentrin SUMO-specific protease 2)
Gene Names
SENP2; AXAM2; SMT3IP2; KIAA1331; DKFZp762A2316
Reactivity
Human, Mouse
Applications
WB
Purity
Purified
Purified
Pricing
$560/0.1 mg | $2,505/5x0.1 mg
Western Blot (WB) (Western blot analysis of UBE2I expression in HeLa cell lysate (MBS179824).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-UBE2I monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for UBE2I )

UBE2I/Ubc9, Monoclonal Antibody (Cat# AAA179824)

Full Name
Anti-UBE2I/Ubc9 Rabbit Monoclonal Antibody
Gene Names
UBE2I; P18; UBC9; C358B7.1
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, IP, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB) (Western blot analysis of UBA1 expression in Jurkat cell lysate (MBS179897).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-UBA1 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for UBA1 )

UBA1/Ube1, Monoclonal Antibody (Cat# AAA179897)

Full Name
Anti-UBA1/Ube1 Rabbit Monoclonal Antibody
Gene Names
UBA1; A1S9; A1ST; GXP1; UBE1; A1S9T; AMCX1; POC20; SMAX2; UBA1A; UBE1X; CFAP124
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB) (Western blot analysis of TNFAIP3 expression in Jurkat cell treated with TNF + TPA lysate (MBS179203).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TNFAIP3 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for TNFAIP3 )

TNFAIP3/A20, Monoclonal Antibody (Cat# AAA179203)

Full Name
Anti-TNFAIP3/A20 Rabbit Monoclonal Antibody
Gene Names
TNFAIP3; A20; AISBL; OTUD7C; TNFA1P2
Reactivity
Human
Applications
FC/FACS, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB) (Western blot analysis of UCHL3 expression in 293T cell lysate (MBS1750033).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-UCHL3 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for UCHL3 )

UCHL3, Monoclonal Antibody (Cat# AAA1750033)

Full Name
Anti-UCHL3 Rabbit Monoclonal Antibody
Gene Names
UCHL3; UCH-L3
Reactivity
Human, Mouse, Rat
Applications
WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB) (Western blot analysis of PIAS1+PIAS2+PIAS3 expression in HepG2 cell lysate (MBS179723).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PIAS1 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PIAS1 )

PIAS1+PIAS2+PIAS3, Monoclonal Antibody (Cat# AAA179723)

Full Name
Anti-PIAS1+PIAS2+PIAS3 Rabbit Monoclonal Antibody
Gene Names
PIAS1; GBP; ZMIZ3; DDXBP1; GU/RH-II
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, IP, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB) (Western blot analysis of USP14 expression in (1) HeLa cell lysate; (2) RAW 2647 cell lysate; (3) C6 cell lysate (MBS179929).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-USP14 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for USP14 )

USP14, Monoclonal Antibody (Cat# AAA179929)

Full Name
Anti-USP14 Rabbit Monoclonal Antibody
Gene Names
USP14; TGT
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, IF, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB) (Western blot analysis of CDC34 expression in (1) Jurkat cell lysate; (2) NIH/3T3 cell lysate (MBS179928).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDC34 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CDC34 )

CDC34/Ubch3, Monoclonal Antibody (Cat# AAA179928)

Full Name
Anti-CDC34/Ubch3 Rabbit Monoclonal Antibody
Gene Names
CDC34; UBC3; UBCH3; UBE2R1; E2-CDC34
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, IP, IF, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
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