Stromal Cell Derived Factor 4 (SDF4) Recombinant Protein | SDF4 recombinant protein

Recombinant Stromal Cell Derived Factor 4 (SDF4)

Cat. Num. AAA2012110

• Gene Names: SDF4; Cab45; SDF-4; RP5-902P8.6
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Stromal Cell Derived Factor 4 (SDF4); Recombinant Stromal Cell Derived Factor 4 (SDF4); SDF4 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.; MGHHHHHHSGSEF- DGHVSWD EYKVKFLASK GHSEKEVADA IRLNEELKVD EETQEVLENL KDRWYQADSP PADLLLTEEE FLSFLHPEHS RGMLRFMVKE IVRDLDQDGD KQLSVPEFIS LPVGTVENQQ GQDIDDNWVK DRKKEFEELI DSNHDGIVTA EELESYMDPM NEYNALNEAK QMIAVADENQ NHHLEPEEVL KYSEFFTGSK LVDYARSVHE EF
• Sequence Length: 362

• Applicable Applications for SDF4 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Homo sapiens (Human)
• Expression System: Prokaryotic expression
• Residues: Asp154~Phe362 (Accession # Q9BRK5) with N-terminal His-Tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 18699732
• NCBI GeneID: 51150
• NCBI Accession #: NP_057260.2
• NCBI GenBank Nucleotide #: NM_016176.3
• UniProt Accession #: Q9BRK5; Q53G52; Q53HQ9; Q8NBQ3; Q96AA1; Q9NZP7; Q9UN53; B1AME5; B1AME6; B2RDF1; B4DSM1
• Molecular Weight: 25.8kDa
• NCBI Official Full Name: 45 kDa calcium-binding protein isoform 2
• NCBI Official Synonym Full Names: stromal cell derived factor 4
• NCBI Official Symbol: SDF4
• NCBI Official Synonym Symbols: Cab45; SDF-4; RP5-902P8.6
• NCBI Protein Information: 45 kDa calcium-binding protein
• UniProt Protein Name: 45 kDa calcium-binding protein
• Protein Family: 45 kDa calcium-binding protein
• UniProt Gene Name: SDF4
• UniProt Synonym Gene Names: CAB45; Cab45; SDF-4
• UniProt Entry Name: CAB45_HUMAN

NCBI Description

This gene encodes a stromal cell derived factor that is a member of the CREC protein family. The encoded protein contains six EF-hand motifs and calcium-binding motifs. This protein localizes to the Golgi lumen and may be involved in regulating calcium dependent cellular activities. [provided by RefSeq, Sep 2011]

Uniprot Description

SDF4: May regulate calcium-dependent activities in the endoplasmic reticulum lumen or post-ER compartment. Belongs to the CREC family. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: Calcium-binding

Chromosomal Location of Human Ortholog: 1p36.33

Cellular Component: Golgi apparatus; membrane; Golgi lumen; late endosome; cytoplasm; plasma membrane

Molecular Function: identical protein binding; protein binding; calcium ion binding

Biological Process: fat cell differentiation; response to ethanol; UV protection; cerebellum development; calcium ion-dependent exocytosis