SCYL3 recombinant protein

SCYL3 recombinant protein

Cat. Num. AAA515257

• Gene Names: SCYL3; PACE1; PACE-1
• Applications: Western Blot
• Synonyms: SCYL3; SCYL3 recombinant protein; PACE-1; PACE1; RP1-97P20.2

• Host: Sf9 insect cells
• Form/Format: 50mM Tris-HCl, pH 7.5, 50mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
• Sequence Length: 2874

• Applicable Applications for SCYL3 recombinant protein: Western Blot (WB)
• Type: Recombinant Fusion Protein
• Species: Human
• Tag Information: GST tag
• Expression System: Sf9 insect cells using baculovirus
• Source Note: Recombinant human SCYL3 (2-end) was expressed by baculovirus in Sf9 insect cells
• Preparation and Storage: Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

SDS-PAGE

Related Product Information for SCYL3 recombinant protein

Recombinant human SCYL3 (2-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Scientific Background: SCYL3 encodes a protein with a kinase domain and four HEAT repeats which interact with the C-terminal domain of ezrin, an ERM protein which play a role in cell adhesion and migration (1). C-terminal domain of SCYL3 interacts with the C-terminal alpha-helical domain of ezrin. Biochemical assays indicate that SCYL3 had associated rather than intrinsic protein kinase activity. SCYL3 is associated with the cytoplasmic face of the Golgi apparatus. This distribution is dependent upon the presence of the SCYL3 N-terminal myristoylation consensus sequence but is not dependent on an association with ezrin. SCYL3 is highly expressed in breast carcinoma cells and adult colon.

Product Categories/Family for SCYL3 recombinant protein

Cellular Proteins; Signaling Proteins - Cellular Proteins

References

1. Sullivan, A. et.al: PACE-1, a novel protein that interacts with the C-terminal domain of ezrin. Exp. Cell Res. 284: 224-238, 2003.

NCBI and Uniprot Product Information

• NCBI GI #: 15779206
• NCBI GeneID: 57147
• UniProt Accession #: Q8IZE3; Q5THA6; Q5THA8; Q8IZN9; Q96C56; Q9UBK6; A8K8Z2
• Molecular Weight: ~110 kDa
• NCBI Official Full Name: Homo sapiens SCY1-like 3 (S. cerevisiae), mRNA
• NCBI Official Synonym Full Names: SCY1-like 3 (S. cerevisiae)
• NCBI Official Symbol: SCYL3
• NCBI Official Synonym Symbols: PACE1; PACE-1
• NCBI Protein Information: protein-associating with the carboxyl-terminal domain of ezrin; SCY1-like protein 3; ezrin-binding protein PACE-1; ezrin-binding partner PACE-1 (PACE-1)
• UniProt Protein Name: Protein-associating with the carboxyl-terminal domain of ezrin
• Protein Family: Protein-associating with the carboxyl-terminal domain of ezrin
• UniProt Gene Name: SCYL3
• UniProt Synonym Gene Names: PACE1
• UniProt Entry Name: PACE1_HUMAN

NCBI Description

This gene encodes a protein with a kinase domain and four HEAT repeats. The encoded protein interacts with the C-terminal domain of ezrin, an ERM protein, and may play a role in cell adhesion and migration. Alternative splicing results in multiple transcript variants encoding multiple isoforms. [provided by RefSeq, Jun 2012]

Uniprot Description

Function: May play a role in regulating cell adhesion/migration complexes in migrating cells. Ref.1

Subunit structure: Interacts with EZR/VIL2 C-terminal domain. Ref.1

Subcellular location: Cytoplasm. Golgi apparatus. Cell projection › lamellipodium. Note: Colocalized with EZR/VIL2, actin and CD44 in lamellipodia. Ref.1

Tissue specificity: Ubiquitously expressed. Ref.1

Domain: The protein kinase domain is predicted to be catalytically inactive.

Post-translational modification: May be myristoylated; myristoylation may target it to Golgi compartment.Phosphorylated. Ref.1

Sequence similarities: Belongs to the protein kinase superfamily.Contains 4 HEAT repeats.Contains 1 protein kinase domain.