SCN2B recombinant protein

SCN2B Protein, Human, Recombinant (hFc Tag)

Cat. Num. AAA8121557

• Purity: >90% as determined by SDS-PAGE
• Synonyms: SCN2B; SCN2B Protein; Human; Recombinant (hFc Tag); Human SCN2B Protein (Fc Tag); ATFB14 Protein; sodium channel; voltage gated; type II beta subunit; SCN2B recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Ala159

• Species: Human
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Met 30
• Tag: C-human IgG1-Fc
• Protein Construction: A DNA sequence encoding the human SCN2B (O60939)(Met1-Ala159) was expressed, fused with the Fc region of human IgG1 at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for SCN2B recombinant protein

Background: SCN2B plays a key role in the assembly, expression, and functional modulation of the heterotrimeric complex of the sodium channel. Voltage-gated sodium channels (NaV) are composed of one pore-forming alpha-subunit, which may be associated with either one or more beta-subunits. Alpha-subunits are composed for four homologous domains, each of which contains six transmembrane segments. They are responsible for action potential initiation and propagation in excitable cells, including nerve, muscle, and neuroendocrine cell types. SCN2B causes an increase in the plasma membrane surface area and in its folding into microvilli. SCN2B also interacts with TNR and may play a crucial role in clustering and regulation of activity of sodium channels at nodes of ranvier.

References

Kimura K, et al. (2006) Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes. Genome Res. 16(1):55-65.Tan BH, et al. (2010) Sudden infant death syndrome-associated mutations in the sodium channel beta subunits. Heart Rhythm. 7(6):771-8.Watanabe H, et al. (2009) Mutations in sodium channel beta1- and beta2-subunits associated with atrial fibrillation. Circ Arrhythm Electrophysiol. 2(3):268-75. Kimura K et al., 2006, Genome Res. 16(1):55-65.

NCBI and Uniprot Product Information

• NCBI GI #: 4759066
• NCBI GeneID: 6327
• NCBI Accession #: NP_004579.1
• NCBI GenBank Nucleotide #: NM_004588.4
• UniProt Accession #: O60939; O75302; Q9UNN3
• Molecular Weight: 24,326 Da
• NCBI Official Full Name: sodium channel subunit beta-2
• NCBI Official Synonym Full Names: sodium channel, voltage-gated, type II, beta subunit
• NCBI Official Symbol: SCN2B
• NCBI Protein Information: sodium channel subunit beta-2; sodium channel beta 2 subunit; neuronal voltage-gated sodium channel beta 2 subunit; sodium channel, voltage-gated, type II, beta polypeptide
• UniProt Protein Name: Sodium channel subunit beta-2
• Protein Family: Sodium channel
• UniProt Gene Name: SCN2B
• UniProt Entry Name: SCN2B_HUMAN

NCBI Description

The protein encoded by this gene is the beta 2 subunit of the type II voltage-gated sodium channel. The encoded protein is involved in cell-cell adhesion and cell migration. Defects in this gene can be a cause of Brugada Syndrome, atrial fibrillation, or sudden infant death syndrome. [provided by RefSeq, Jul 2015]

Uniprot Description

SCN2B: Crucial in the assembly, expression, and functional modulation of the heterotrimeric complex of the sodium channel. The subunit beta-2 causes an increase in the plasma membrane surface area and in its folding into microvilli. Interacts with TNR may play a crucial role in clustering and regulation of activity of sodium channels at nodes of Ranvier. Belongs to the sodium channel auxiliary subunit SCN2B (TC 8.A.17) family.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 11q23

Cellular Component: voltage-gated sodium channel complex

Molecular Function: sodium channel regulator activity

Biological Process: nervous system development; synaptic transmission; cardiac muscle contraction; response to pyrethroid

Disease: Atrial Fibrillation, Familial, 14