SARS-CoV-2 Nucleocapsid Recombinant Protein | NP-CoV recombinant protein

SARS-CoV-2 Nucleocapsid (A119S, R203K, G204R, M234I) Protein (His Tag)

Cat. Num. AAA8122247

• Purity: >90% as determined by SDS-PAGE
• Synonyms: SARS-CoV-2 Nucleocapsid; SARS-CoV-2 Nucleocapsid (A119S; R203K; G204R; M234I) Protein (His Tag); NP-CoV recombinant protein

• Host: E Coli
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 50mM PB, 500mM NaCl, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Ala419

• Species: SARS-CoV-2
• Predicted N Terminal: Met
• Tag: N-His
• Protein Construction: A DNA sequence encoding the SARS-CoV-2 Nucleocapsid (YP_009724397.2, with mutations A119S, R203K, G204R, M234I)(Met1-Ala419) was expressed with a polyhistidine tag at the N-terminus. The mutations were identified in the SARS-CoV-2 variant (known as variant P.2) which emerged in the Brazil.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for NP-CoV recombinant protein

Background: Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. The coronavirus N protein is required for coronavirus RNA synthesis and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is the most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to the formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of the N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a test tool.

References

1.Van Boheemen S, et al. (2012), MBio. 3(6):e00473-12. Bisht H. et al., 2004, Proc Natl Acad Sci. 101 (17): 6641-6. Li W. et al., 2005, Science. 309 (5742): 1864-8.

NCBI and Uniprot Product Information

• NCBI GI #: 1798174255
• NCBI Accession #: YP_009724397.2
• NCBI GenBank Nucleotide #: YP_009724397.2
• Molecular Weight: 47.08 kDa
• NCBI Official Full Name: nucleocapsid phosphoprotein