SAE1 recombinant protein

SAE1, 1-346aa, Human, His-T7 tag, E Coli

Cat. Num. AAA203729

• Gene Names: SAE1; AOS1; SUA1; UBLE1A; HSPC140
• Applications: SDS-Page
• Purity: > 90% by SDS-PAGE
• Synonyms: SAE1; 1-346aa; Human; His-T7 tag; E Coli; SuMO1 activating enzyme subunit 1; AOS1; HSPC140; SuA1; uBLE1A; ubiquitin-like 1-activating enzyme E1A; AOS 1; HSPC 140; SuA 1; uBLE 1A; uBL E1A; SAE 1; ubiquitin like protein SuMO 1 activating enzyme; SAE1 recombinant protein

• Host: E Coli
• Purity/Purification: > 90% by SDS-PAGE
• Form/Format: Liquid. In 20 mM Tris-HCl Buffer (pH 8.0) containing 1mM DTT, 10% glycerol
• Concentration: 1 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MHHHHHHMAS MTGGQQMGRD LYDDDDKDRW GSMVEKEEAG GGISEEEAAQ YDRQIRLWGL EAQKRLRASR VLLVGLKGLG AEIAKNLILA GVKGLTMLDH EQVTPEDPGA QFLIRTGSVG RNRAEASLER AQNLNPMVDV KVDTEDIEKK PESFFTQFDA VCLTCCSRDV IVKVDQICHK NSIKFFTGDV FGYHGYTFAN LGEHEFVEEK TKVAKVSQGV EDGPDTKRAK LDSSETTMVK KKVVFCPVKE ALEVDWSSEK AKAALKRTTS DYFLLQVLLK FRTDKGRDPS SDTYEEDSEL LLQIRNDVLD SLGISPDLLP EDFVRYCFSE MAPVCAVVGG ILAQEIVKAL SQRDPPHNNF FFFDGMKGNG IVECLGPK
• Sequence Length: 1-346

• Applicable Applications for SAE1 recombinant protein: SDS-PAGE
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 2-8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Avoid repeated freezing and thawing cycles.

SDS-Page

(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.)

Related Product Information for SAE1 recombinant protein

SAE1, also known as AOS1, belongs to the ubiquitin-activating E1 family of proteins and plays an important role in the first step of the uBL1 conjugation pathway. Proteins conjugated to ub are marked for progressive degradation by the 26S Proteasome. SAE1, which is a dimeric enzyme, functions as a uBLI E1 ligase mediating the ATP-dependent activation of uBL1. This protein can bind with uBLE1A and uBLE1B to form a heterodimer which can bind uBL1. Recombinant human SAE1 protein, fused to His-T7-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for SAE1 recombinant protein

Ubiquitination

References

Boggio R., et al. (2007) J Biol Chem. 282 (21):15376-82.; Jones MC., et al. (2006) Proc Natl Acad Sci u S A. 103 (44):16272-7.;

NCBI and Uniprot Product Information

• NCBI GI #: 4885585
• NCBI GeneID: 10055
• NCBI Accession #: NP_005491
• NCBI GenBank Nucleotide #: NM_005500.2
• UniProt Accession #: Q9UBE0; O95717; Q9P020; B2RDP5; B3KMQ2; F5GXX7; G3XAK6
• Molecular Weight: 42.2 kDa (378aa) confirmed by MALDI-TOF
• NCBI Official Full Name: SUMO-activating enzyme subunit 1 isoform a
• NCBI Official Synonym Full Names: SUMO1 activating enzyme subunit 1
• NCBI Official Symbol: SAE1
• NCBI Official Synonym Symbols: AOS1; SUA1; UBLE1A; HSPC140
• NCBI Protein Information: SUMO-activating enzyme subunit 1
• UniProt Protein Name: SUMO-activating enzyme subunit 1
• Protein Family: SUMO-activating enzyme
• UniProt Gene Name: SAE1
• UniProt Synonym Gene Names: AOS1; SUA1; UBLE1A

NCBI Description

Posttranslational modification of proteins by the addition of the small protein SUMO (see SUMO1; MIM 601912), or sumoylation, regulates protein structure and intracellular localization. SAE1 and UBA2 (MIM 613295) form a heterodimer that functions as a SUMO-activating enzyme for the sumoylation of proteins (Okuma et al., 1999 [PubMed 9920803]).[supplied by OMIM, Mar 2010]

Uniprot Description

The heterodimer acts as an E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2.