Mouse Sphingosine 1 Phosphate Receptor 4 ELISA Kit | S1PR4 elisa kit

Mouse Sphingosine 1 Phosphate Receptor 4 ELISA Kit

Cat. Num. AAA764283

• Gene Names: S1PR4; EDG6; LPC1; S1P4; SLP4
• Reactivity: Mouse
• Synonyms: Sphingosine 1 Phosphate Receptor 4; Mouse Sphingosine 1 Phosphate Receptor 4 ELISA Kit; S1PR4/EDG6/SLP4/Endothelial differentiation G-protein coupled receptor 6/S1P receptor 4//sphingosine 1-phosphate receptor 4/Sphingosine 1-phosphate receptor Edg-6/sphingosine-1-phosphate receptor 4; S1PR4 elisa kit

• Reactivity: Mouse
• Sequence Length: 384

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 78.125-5000pg/ml
• Sensitivity: 46.875pg/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for S1PR4 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology.Anti-Mouse S1PR4 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Mouse S1PR4 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse S1PR4 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Mouse S1PR4 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 4503459
• NCBI GeneID: 8698
• NCBI Accession #: NP_003766.1
• NCBI GenBank Nucleotide #: NM_003775.3
• Molecular Weight: 41,623 Da
• NCBI Official Full Name: sphingosine 1-phosphate receptor 4
• NCBI Official Synonym Full Names: sphingosine-1-phosphate receptor 4
• NCBI Official Symbol: S1PR4
• NCBI Official Synonym Symbols: EDG6; LPC1; S1P4; SLP4
• NCBI Protein Information: sphingosine 1-phosphate receptor 4
• UniProt Protein Name: Sphingosine 1-phosphate receptor 4
• Protein Family: Sphingosine 1-phosphate receptor
• UniProt Gene Name: S1PR4
• UniProt Synonym Gene Names: EDG6; S1P receptor 4; S1P4; S1P receptor Edg-6
• UniProt Entry Name: S1PR4_HUMAN

NCBI Description

This gene is a member of the endothelial differentiation, G-protein-coupled (EDG)) receptor gene family. EDG receptors bind lysophospholipids or lysosphingolipids as ligands, and are involved in cell signalling in many different cell types. This EDG receptor gene is intronless and is specifically expressed in the lymphoid tissue. [provided by RefSeq, Jul 2008]

Uniprot Description

S1PR4: Receptor for the lysosphingolipid sphingosine 1- phosphate (S1P). S1P is a bioactive lysophospholipid that elicits diverse physiological effect on most types of cells and tissues. May be involved in cell migration processes that are specific for lymphocytes. Belongs to the G-protein coupled receptor 1 family.

Protein type: Membrane protein, multi-pass; GPCR, family 1; Receptor, GPCR; Membrane protein, integral

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: cytosol; integral to plasma membrane; plasma membrane

Molecular Function: G-protein coupled receptor activity; lipid binding

Biological Process: elevation of cytosolic calcium ion concentration; G-protein coupled receptor protein signaling pathway; G-protein signaling, adenylate cyclase activating pathway; immune response; phospholipase C activation