Chicken S100 calcium binding protein-beta ELISA Kit | S100beta elisa kit

Chicken S100 calcium binding protein-beta ELISA Kit

Cat. Num. AAA739388

• Gene Names: S100B; NEF; S100; S100-B; S100beta
• Reactivity: Chicken
• Synonyms: S100 calcium binding protein-beta; Chicken S100 calcium binding protein-beta ELISA Kit; S100beta elisa kit

• Reactivity: Chicken
• Sequence Length: 92

• Samples: Serum, Plasma, Cell Culture Supernatants, Body Fluid And Tissue Homogenate
• Assay Type: Quantitative Competitive
• Sensitivity: 1.0 pg/mL.
• Preparation and Storage: Store all reagents at 2-8 degree C

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for S100beta elisa kit

Intended Uses: This S100A9 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse S100A9. This ELISA kit for research use only!

Principle of the Assay: S100A9 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-S100A9 antibody and an S100A9-HRP conjugate. The assay sample and buffer are incubated together with S100A9-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the S100A9 concentration since S100A9 from samples and S100A9-HRP conjugate compete for the anti-S100A9 antibody binding site. Since the number of sites is limited, as more sites are occupied by S100A9 from the sample, fewer sites are left to bind S100A9-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The S100A9 concentration in each sample is interpolated from this standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 337730
• NCBI GeneID: 6285
• UniProt Accession #: P04271; D3DSN6
• Molecular Weight: 10,713 Da
• NCBI Official Full Name: S100 protein beta subunit
• NCBI Official Synonym Full Names: S100 calcium binding protein B
• NCBI Official Symbol: S100B
• NCBI Official Synonym Symbols: NEF; S100; S100-B; S100beta
• NCBI Protein Information: protein S100-B; S-100 protein subunit beta; S-100 calcium-binding protein, beta chain; S100 calcium-binding protein, beta (neural)
• UniProt Protein Name: Protein S100-B
• UniProt Gene Name: S100B
• UniProt Entry Name: S100B_HUMAN

NCBI Description

The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21; however, this gene is located at 21q22.3. This protein may function in Neurite extension, proliferation of melanoma cells, stimulation of Ca2+ fluxes, inhibition of PKC-mediated phosphorylation, astrocytosis and axonal proliferation, and inhibition of microtubule assembly. Chromosomal rearrangements and altered expression of this gene have been implicated in several neurological, neoplastic, and other types of diseases, including Alzheimer's disease, Down's syndrome, epilepsy, amyotrophic lateral sclerosis, melanoma, and type I diabetes. [provided by RefSeq, Jul 2008]

Uniprot Description

S100B: Weakly binds calcium but binds zinc very tightly- distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites. Binds to and initiates the activation of STK38 by releasing autoinhibitory intramolecular interactions within the kinase. Interaction with AGER after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling. Dimer of either two alpha chains, or two beta chains, or one alpha and one beta chain. The S100B dimer binds two molecules of STK38. Interacts with AGER. The S100B dimer interacts with two molecules of CAPZA1. Although predominant among the water-soluble brain proteins, S100 is also found in a variety of other tissues. Belongs to the S-101 family.

Protein type: Calcium-binding

Chromosomal Location of Human Ortholog: 21q22.3

Cellular Component: ruffle; extracellular space; cell soma; intracellular membrane-bound organelle; perinuclear region of cytoplasm; cytoplasm; extracellular region; nucleus

Molecular Function: identical protein binding; protein binding; RAGE receptor binding; protein homodimerization activity; zinc ion binding; calcium ion binding; tau protein binding; calcium-dependent protein binding

Biological Process: positive regulation of I-kappaB kinase/NF-kappaB cascade; central nervous system development; positive regulation of apoptosis; response to glucocorticoid stimulus; memory; cell proliferation; regulation of cell shape; learning and/or memory; astrocyte differentiation; axonogenesis; response to methylmercury; positive regulation of cell proliferation; innate immune response; regulation of neuronal synaptic plasticity