S100 recombinant protein

S100 protein (Bovine)

Cat. Num. AAA537934

• Gene Names: S100B; NEF; S100; S100-B; S100beta
• Applications: Immunohistochemistry, immunoassay, Radioimmunoassay, Western Blot
• Synonyms: S100; S100 protein (Bovine); SB-100 protein; SB 100; SB 100 protein; S-100 BB Protein; S-100 Protein; S100B; SB-100; S100 recombinant protein

• Host: Bovine
• Form/Format: Lyophilized from 10mM TRIS-HCl, with 1mM EGTA.
• Sequence Length: 92

• Applicable Applications for S100 recombinant protein: Immunohistochemistry (IHC), Radioimmunoassay (RIA), Western Blot (WB)
• Protein Type: Recombinant
• Biological Significance: S100 calcium binding protein B or S100B is a protein of the S-100 protein family. S100 proteins are localized in the cytoplasm and nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21; however, this gene is located at 21q22.3.
• Expression System: E Coli
• Preparation and Storage: Store at 4 degree C until reconstitution. Following reconstitution aliquot and freeze at -80 degree C for long term storage. Avoid repeated freeze/thaw cycles.

Related Product Information for S100 recombinant protein

Purified Recombinant S100 protein (Bovine)

Product Categories/Family for S100 recombinant protein

Cancer

NCBI and Uniprot Product Information

• NCBI GI #: 119629675
• NCBI GeneID: 6285
• UniProt Accession #: P04271; D3DSN6
• Molecular Weight: 10,713 Da
• NCBI Official Full Name: S100 calcium binding protein, beta (neural), isoform CRA_a
• NCBI Official Synonym Full Names: S100 calcium binding protein B
• NCBI Official Symbol: S100B
• NCBI Official Synonym Symbols: NEF; S100; S100-B; S100beta
• NCBI Protein Information: protein S100-B
• UniProt Protein Name: Protein S100-B
• Protein Family: S100P-binding protein
• UniProt Gene Name: S100B
• UniProt Entry Name: S100B_HUMAN

NCBI Description

The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21; however, this gene is located at 21q22.3. This protein may function in Neurite extension, proliferation of melanoma cells, stimulation of Ca2+ fluxes, inhibition of PKC-mediated phosphorylation, astrocytosis and axonal proliferation, and inhibition of microtubule assembly. Chromosomal rearrangements and altered expression of this gene have been implicated in several neurological, neoplastic, and other types of diseases, including Alzheimer's disease, Down's syndrome, epilepsy, amyotrophic lateral sclerosis, melanoma, and type I diabetes. [provided by RefSeq, Jul 2008]

Uniprot Description

S100B: Weakly binds calcium but binds zinc very tightly- distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites. Binds to and initiates the activation of STK38 by releasing autoinhibitory intramolecular interactions within the kinase. Interaction with AGER after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling. Dimer of either two alpha chains, or two beta chains, or one alpha and one beta chain. The S100B dimer binds two molecules of STK38. Interacts with AGER. The S100B dimer interacts with two molecules of CAPZA1. Although predominant among the water-soluble brain proteins, S100 is also found in a variety of other tissues. Belongs to the S-101 family.

Protein type: Calcium-binding

Chromosomal Location of Human Ortholog: 21q22.3

Cellular Component: ruffle; extracellular space; cell soma; intracellular membrane-bound organelle; perinuclear region of cytoplasm; cytoplasm; extracellular region; nucleus

Molecular Function: identical protein binding; protein binding; RAGE receptor binding; protein homodimerization activity; zinc ion binding; calcium ion binding; calcium-dependent protein binding; tau protein binding

Biological Process: positive regulation of I-kappaB kinase/NF-kappaB cascade; central nervous system development; positive regulation of apoptosis; response to glucocorticoid stimulus; memory; regulation of cell shape; cell proliferation; learning and/or memory; axonogenesis; astrocyte differentiation; positive regulation of cell proliferation; response to methylmercury; innate immune response; regulation of neuronal synaptic plasticity