Chicken S100 Calcium Binding Protein A6 ELISA Kit | S100A6 elisa kit

Chicken S100 Calcium Binding Protein A6 ELISA Kit

Cat. Num. AAA743712

• Gene Names: S100A6; 2A9; PRA; 5B10; CABP; CACY
• Reactivity: Chicken
• Synonyms: S100 Calcium Binding Protein A6; Chicken S100 Calcium Binding Protein A6 ELISA Kit; S100A6 elisa kit

• Reactivity: Chicken

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 0.1 ng/mL.
• Intended Uses: This S100A6 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Chicken S100A6. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for S100A6 elisa kit

Principle of the Assay: S100A6 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-S100A6 antibody and an S100A6-HRP conjugate. The assay sample and buffer are incubated together with S100A6-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the S100A6 concentration since S100A6 from samples and S100A6-HRP conjugate compete for the anti-S100A6 antibody binding site. Since the number of sites is limited, as more sites are occupied by S100A6 from the sample, fewer sites are left to bind S100A6-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The S100A6 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for S100A6 elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 55962767
• NCBI GeneID: 6277
• UniProt Accession #: P06703; Q5RHS4; D3DV39
• Molecular Weight: 10,180 Da
• NCBI Official Full Name: S100 calcium binding protein A6
• NCBI Official Synonym Full Names: S100 calcium binding protein A6
• NCBI Official Symbol: S100A6
• NCBI Official Synonym Symbols: 2A9; PRA; 5B10; CABP; CACY
• NCBI Protein Information: protein S100-A6; MLN 4; calcyclin; growth factor-inducible protein 2A9; prolactin receptor-associated protein; S100 calcium-binding protein A6 (calcyclin)
• UniProt Protein Name: Protein S100-A6
• Protein Family: Protein
• UniProt Gene Name: S100A6
• UniProt Synonym Gene Names: CACY; PRA
• UniProt Entry Name: S10A6_HUMAN

NCBI Description

The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in stimulation of Ca2+-dependent insulin release, stimulation of prolactin secretion, and exocytosis. Chromosomal rearrangements and altered expression of this gene have been implicated in melanoma. [provided by RefSeq, Jul 2008]

Uniprot Description

S100A6: May function as calcium sensor and contribute to cellular calcium signaling (Potential). May function by interacting with other proteins and indirectly play a role in the reorganization of the actin cytoskeleton and in cell motility. Binds 2 calcium ions. Calcium binding is cooperative. Belongs to the S-100 family.

Chromosomal Location of Human Ortholog: 1q21

Cellular Component: ruffle; extrinsic to internal side of plasma membrane; perinuclear region of cytoplasm; cytoplasm; nuclear envelope; cytosol; nucleus

Molecular Function: protein binding; protein homodimerization activity; zinc ion binding; ion transmembrane transporter activity; calcium ion binding; tropomyosin binding; calcium-dependent protein binding

Biological Process: positive regulation of fibroblast proliferation; axonogenesis; signal transduction