Human Ribosome-binding protein 1 ELISA Kit | RRBP1 elisa kit

Human Ribosome-binding protein 1 ELISA Kit

Cat. Num. AAA9426533

• Gene Names: RRBP1; RRp; hES; ES130; ES/130
• Reactivity: Human
• Synonyms: Ribosome-binding protein 1; Human Ribosome-binding protein 1 ELISA Kit; 180 kDa ribosome receptor homolog; RRp; ES/130-related protein; Ribosome receptor protein; RRBP1; KIAA1398; RRBP1 elisa kit

• Reactivity: Human
• Specificity: This assay recognizes recombinant and natural human RRBP1. No significant cross-reactivity or interference was observed.

• Samples: Serum, plasma, tissue homogenates and cell culture supernates and other biological fluids
• Detection Range: 78.0 pg/mL -5000 pg/mL
• Preparation and Storage: Store at 4 degree C

Typical Testing Data/Standard Curve (for reference only)

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Related Product Information for RRBP1 elisa kit

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of human ribosome-binding protein 1,RRBP1 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to RRBP1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for RRBP1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain RRBP1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzymesubstrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of RRBP1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 110611218
• NCBI GeneID: 6238
• NCBI Accession #: NP_001036041.1
• NCBI GenBank Nucleotide #: NM_001042576.1
• UniProt Accession #: Q9P2E9; O75300; O75301; Q5W165; Q96SB2; Q9BWP1; Q9H476; A2A2S6; A6NCN6
• Molecular Weight: 108,632 Da
• NCBI Official Full Name: ribosome-binding protein 1
• NCBI Official Synonym Full Names: ribosome binding protein 1
• NCBI Official Symbol: RRBP1
• NCBI Official Synonym Symbols: RRp; hES; ES130; ES/130
• NCBI Protein Information: ribosome-binding protein 1
• UniProt Protein Name: Ribosome-binding protein 1
• Protein Family: Ribosome-binding protein
• UniProt Gene Name: RRBP1
• UniProt Synonym Gene Names: KIAA1398; RRp

NCBI Description

This gene encodes a ribosome-binding protein of the endoplasmic reticulum (ER) membrane. Studies suggest that this gene plays a role in ER proliferation, secretory pathways and secretory cell differentiation, and mediation of ER-microtubule interactions. Alternative splicing has been observed and protein isoforms are characterized by regions of N-terminal decapeptide and C-terminal heptad repeats. Splicing of the tandem repeats results in variations in ribosome-binding affinity and secretory function. The full-length nature of variants which differ in repeat length has not been determined. Pseudogenes of this gene have been identified on chromosomes 3 and 7, and RRBP1 has been excluded as a candidate gene in the cause of Alagille syndrome, the result of a mutation in a nearby gene on chromosome 20p12. [provided by RefSeq, Apr 2012]

Uniprot Description

RRBP1: a single-pass type III membrane protein of the rough endoplasmic reticulum membrane. Acts as a ribosome receptor and mediates interaction between the ribosome and the endoplasmic reticulum membrane. Associated with the unfolded protein response (UPR) and has anti-apoptotic activity. Knockdown of RRBP1 induces ER stress, and is associated with a significant reduction in the expression of glucose-regulated protein 78 (GRP78). GRP78 regulates the UPR, is widely upregulated in cancer, and plays an important role in chemotherapy resistance in some cancers. Upregulated in many cancers: overexpressed in a high proportion of breast carcinoma cases tested, and is highly expressed in lung cancer tissue compared with adjacent normal tissues. Because RRBP1 appears to play a role in tumor cell survival, it may be a candidate for new targeted therapeutics. Three human isoforms are produced by alternative splicing.

Protein type: Membrane protein, integral; Ribosomal; Translation

Chromosomal Location of Human Ortholog: 20p12.1

Cellular Component: endoplasmic reticulum; integral to endoplasmic reticulum membrane; membrane; ribosome

Molecular Function: receptor activity; RNA binding

Biological Process: osteoblast differentiation; translation