26S protease regulatory subunit 6A homolog A (RPT5A) Recombinant Protein | RPT5A recombinant protein

Recombinant Arabidopsis thaliana 26S protease regulatory subunit 6A homolog A (RPT5A)

Cat. Num. AAA1438245

• Gene Names: RPT5A; 19S PROTEASOME REGULATORY COMPLEX SUBUNIT S6A; 26S PROTEASOME AAA-ATPASE SUBUNIT; ATS6A.2; F22F7.1; F22F7_1; regulatory particle triple-A ATPase 5A
• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: 26S protease regulatory subunit 6A homolog A (RPT5A); Recombinant Arabidopsis thaliana 26S protease regulatory subunit 6A homolog A (RPT5A); RPT5A recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process)
• Sequence Positions: 2-424, Full length protein
• Sequence: ATPMVEDTSSFEEDQLASMSTEDITRATRLLDNEIRILKEDAQRTNLECDSYKEKIKENQEKIKLNKQLPYLVGNIVEILEMNPEDDAEEDGANIDLDSQRKGKCVVLKTSTRQTIFLPVVGLVDPDSLKPGDLVGVNKDSYLILDTLPSEYDSRVKAMEVDEKPTEDYNDIGGLEKQIQELVEAIVLPMTHKERFEKLGVRPPKGVLLYGPPGTGKTLMARACAAQTNATFLKLAGPQLVQMFIGDGAKLVRDAFQLAKEKAPCIIFIDEIDAIGTKRFDSEVSGDREVQRTMLELLNQLDGFSSDERIKVIAATNRADILDPALMRSGRLDRKIEFPHPTEEARARILQIHSRKMNVHPDVNFEELARSTDDFNGAQLKAVCVEAGMLALRRDATEVNHEDFNEGIIQVQAKKKASLNYYA
• Sequence Length: 423

• Species: Arabidopsis thaliana (Mouse-ear cress)
• Preparation and Storage: Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.

NCBI and Uniprot Product Information

• NCBI GI #: 15230005
• NCBI GeneID: 819718
• NCBI Accession #: NP_187204.1
• NCBI GenBank Nucleotide #: NM_111426.4
• UniProt Accession #: Q9SEI2; Q8H195; Q9SWY7
• Molecular Weight: 47,480 Da
• NCBI Official Full Name: regulatory particle triple-A ATPase 5A
• NCBI Official Symbol: RPT5A
• NCBI Official Synonym Symbols: 19S PROTEASOME REGULATORY COMPLEX SUBUNIT S6A; 26S PROTEASOME AAA-ATPASE SUBUNIT; ATS6A.2; F22F7.1; F22F7_1; regulatory particle triple-A ATPase 5A
• NCBI Protein Information: regulatory particle triple-A ATPase 5A
• UniProt Protein Name: 26S proteasome regulatory subunit 6A homolog A
• Protein Family: 26S protease regulatory
• UniProt Gene Name: RPT5A
• UniProt Synonym Gene Names: ATS6A.2; TBP1; TBP-1 homolog A

NCBI Description

Encodes RPT5a (Regulatory Particle 5a), one of the six AAA-ATPases of the proteasome regulatory particle. Essential for gametophyte development. In Arabidopsis, the RPT5 subunit is encoded by two highly homologous genes, RPT5a and RPT5b. RPT5a and RPT5b show accession-dependent functional redundancy. In Wassilewskija (Ws) accession: mutant alleles of RPT5a displayed 50% pollen lethality, indicating that RPT5a is essential for male gametophyte development. In the Columbia (Col) accession, a rpt5a mutant allele did not display such a phenotype because the RPT5b Col allele complements the rpt5a defect in the male gametophyte, whereas the RPT5b Ws allele does not. Double rpt5a rpt5b mutants in Col background showed a complete male and female gametophyte lethal phenotype.

Uniprot Description

The 26S proteasome is involved in the ATP-dependent degradation of ubiquitinated proteins. The regulatory (or ATPase) complex confers ATP dependency and substrate specificity to the 26S complex. Plays a essential role in the gametophyte development.