RNA polymerase sigma factor rpoS (rpoS) Recombinant Protein | rpoS recombinant protein

Recombinant Escherichia coli RNA polymerase sigma factor rpoS (rpoS)

Cat. Num. AAA7136981

• Gene Names: rpoS; abrD; appR; csi2; dpeB; ECK2736; JW5437; katF; nur; otsX; sigS
• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: RNA polymerase sigma factor rpoS (rpoS); Recombinant Escherichia coli RNA polymerase sigma factor rpoS (rpoS); Sigma SSigma-38; rpoS recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process); If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.; If the delivery form is lyophilized powder, the buffer before lyop
• Sequence Positions: 1-330aa, Full Length
• Sequence: MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEQEPSDNDLAEEELLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLVVKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMNQTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNERITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLARRFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRE

• Species: Escherichia coli (strain K12)
• Tag: N-terminal 6xHis-tagged
• Function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is the master transcriptional regulator of the stationary phase and the general stress response. Controls, positively or negatively, the expression of several hundred genes, which are mainly involved in metabolism, transport, regulation and stress management.
• Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 degree C/-80 degree C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
• Preparation and Storage: Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.

Product Categories/Family for rpoS recombinant protein

Transcription

References

"In vitro transcription profiling of the sigmaS subunit of bacterial RNA polymerase: re-definition of the sigmaS regulon and identification of sigmaS-specific promoter sequence elements."Maciag A., Peano C., Pietrelli A., Egli T., De Bellis G., Landini P.Nucleic Acids Res. 39:5338-5355 (2011)

NCBI and Uniprot Product Information

• NCBI GI #: 16130648
• NCBI GeneID: 947210
• NCBI Accession #: NP_417221
• NCBI GenBank Nucleotide #: NC_000913.2
• UniProt Accession #: P13445; Q2MA88; Q79EB7
• Molecular Weight: 37,972 Da
• NCBI Official Full Name: RNA polymerase, sigma S (sigma 38) factor
• NCBI Official Symbol: rpoS
• NCBI Official Synonym Symbols: abrD; appR; csi2; dpeB; ECK2736; JW5437; katF; nur; otsX; sigS
• NCBI Protein Information: RNA polymerase, sigma S (sigma 38) factor
• UniProt Protein Name: RNA polymerase sigma factor RpoS
• Protein Family: RNA polymerase sigma factor
• UniProt Gene Name: rpoS
• UniProt Synonym Gene Names: appR; katF; nur; otsX; sigS
• UniProt Entry Name: RPOS_ECOLI

NCBI Description

Polyamine modulon. [More information is available at EcoGene: EG10510]. rpoS encodes the alternative sigma factor S, a subunit of RNA polymerase that acts as the master regulator of the general stress response in Escherichia coli. [More information is available at EcoCyc: EG10510].

Uniprot Description

Function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is the master transcriptional regulator of the stationary phase and the general stress response. Controls, positively or negatively, the expression of several hundred genes, which are mainly involved in metabolism, transport, regulation and stress management.

Subunit structure: Interacts with the RNA polymerase core enzyme and RssB.

Subcellular location: Cytoplasm

Potential.

Induction: Subject to complex regulation at multiple levels (transcription, translation, regulation of activity and degradation). Transcription is induced during entry into stationary phase and in response to different stresses. mRNA stability is regulated by small RNA regulators and various proteins such as Hfq, CsdA, CspC and CspE. Finally, the cellular level of RpoS is regulated by proteolysis via RssB and the ClpXP protease.

Sequence similarities: Belongs to the sigma-70 factor family. RpoS subfamily.

Sequence caution: The sequence BAE76818.1 differs from that shown. Reason: Erroneous termination at position 33. Translated as Gln.The sequence CAA34435.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.The sequence CAA78692.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.