RORA recombinant protein

Recombinant Human RORA protein, N- His Tag & sumo Tag

Cat. Num. AAA157696

• Gene Names: RORA; ROR1; ROR2; ROR3; RZRA; NR1F1; RZR-ALPHA
• Purity: >90% as determined by SDS-PAGE
• Synonyms: RORA; Recombinant Human RORA protein; N- His Tag & sumo Tag; Nuclear receptor RZR-alpha; Nuclear receptor subfamily 1 group F member 1; RAR-related orphan receptor A; Retinoid-related orphan receptor-alpha; NR1F1; RZRA; RORA recombinant protein

• Host: E Coli
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized. Lyophilized from PBS pH 7.4, 0.2% NLS, 5% Trehalose, 5% Mannose, 1mM EDTA.

• Source: Human
• Construction: A DNA sequence encoding the Human RORA (Glu263-Gly523) was fused with the N-terminal His Tag & sumo Tag
• Preparation and Storage: Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8 degree C for one week. Store at -20 to -80 degree C for ~12 months from the date of receipt.; Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.; In general, proteins are provided as lyophilized powder. They are shipped out with blue ice unless customers require otherwise

Product Categories/Family for RORA recombinant protein

Protein

NCBI and Uniprot Product Information

• NCBI GI #: 4506577
• NCBI GeneID: 6095
• NCBI Accession #: NP_002934.1
• NCBI GenBank Nucleotide #: NM_002943.3
• UniProt Accession #: P35398; P35397; P35399; P45445; Q495X4; Q96H83
• Molecular Weight: 63,036 Da
• NCBI Official Full Name: nuclear receptor ROR-alpha isoform c
• NCBI Official Synonym Full Names: RAR-related orphan receptor A
• NCBI Official Symbol: RORA
• NCBI Official Synonym Symbols: ROR1; ROR2; ROR3; RZRA; NR1F1; RZR-ALPHA
• NCBI Protein Information: nuclear receptor ROR-alpha; nuclear receptor RZR-alpha; transcription factor RZR-alpha; retinoid-related orphan receptor alpha; nuclear receptor subfamily 1 group F member 1; thyroid hormone nuclear receptor alpha variant 4; retinoic acid receptor-related
• UniProt Protein Name: Nuclear receptor ROR-alpha
• Protein Family: Nuclear receptor
• UniProt Gene Name: RORA
• UniProt Synonym Gene Names: NR1F1; RZRA
• UniProt Entry Name: RORA_HUMAN

NCBI Description

The protein encoded by this gene is a member of the NR1 subfamily of nuclear hormone receptors. It can bind as a monomer or as a homodimer to hormone response elements upstream of several genes to enhance the expression of those genes. The specific functions of this protein are not known, but it has been shown to interact with NM23-2, a nucleoside diphosphate kinase involved in organogenesis and differentiation, as well as with NM23-1, the product of a tumor metastasis suppressor candidate gene. Four transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

RORA iso1: Orphan nuclear receptor. Binds DNA as a monomer to hormone response elements (HRE) containing a single core motif half-site preceded by a short A-T-rich sequence. This isomer binds to the consensus sequence 5'-[AT][TA]A[AT][CGT]TAGGTCA-3'. Regulates a number of genes involved in lipid metabolism such as apolipoproteins AI, APOA5, CIII, CYP71 and PPARgamma, in cerebellum and photoreceptor development including PCP2, OPN1SW, OPN1SM AND ARR3, in circadian rhythm with BMAL1, and skeletal muscle development with MYOD1. Possible receptor for cholesterol or one of its derivatives. Monomer. Interacts (via the DNA-binding domain) with HIF1; the interaction enhances HIF1A transcription under hypoxia through increasing protein stability. By hypoxia and melatonin. Widely expressed in a number of tissues. Activated by CaMK4. Belongs to the nuclear hormone receptor family. NR1 subfamily. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; Nuclear receptor

Chromosomal Location of Human Ortholog: 15q22.2

Cellular Component: nucleoplasm; nucleus

Molecular Function: protein binding; ligand-dependent nuclear receptor activity; DNA binding; zinc ion binding; sequence-specific DNA binding; beta-catenin binding; oxysterol binding; steroid hormone receptor activity; transcription factor activity; transcription factor binding

Biological Process: circadian rhythm; transcription initiation from RNA polymerase II promoter; granule cell precursor proliferation; intracellular receptor-mediated signaling pathway; regulation of macrophage activation; positive regulation of transcription, DNA-dependent; negative regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of circadian rhythm; negative regulation of fat cell differentiation; muscle cell differentiation; regulation of transcription, DNA-dependent; negative regulation of inflammatory response; xenobiotic metabolic process; regulation of steroid metabolic process; cerebellar Purkinje cell differentiation; positive regulation of transcription from RNA polymerase II promoter; steroid hormone mediated signaling; gene expression; angiogenesis; circadian regulation of gene expression; nitric oxide biosynthetic process; cGMP metabolic process; regulation of smoothened signaling pathway