Human Rho Associated Coiled Coil Containing Protein Kinase 2 ELISA Kit | Rock2 elisa kit

Human Rho Associated Coiled Coil Containing Protein Kinase 2 ELISA Kit

Cat. Num. AAA107412

• Gene Names: ROCK2; ROCK-II
• Reactivity: Human
• Synonyms: Rho Associated Coiled Coil Containing Protein Kinase 2; Human Rho Associated Coiled Coil Containing Protein Kinase 2 ELISA Kit; Rock2 elisa kit

• Reactivity: Human
• Specificity: No significant cross-reactivity or interference between Human Rock2 and analogues was observed.
• Sequence Length: 1388

• Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids
• Assay Type: Sandwich
• Detection Range: 6.25 ng/ml - 200 ng/ml.
• Sensitivity: 1.0 ng/ml.
• Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic applications! It is intended to be determinated Rock2 concentrations in Human serum, plasma and other body fluids. Using Purified Human Rock2 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add Rock2 and Rock2 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Rock2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Intra-assay Precision: Intra-assay CV (%) is less than 15%.
• Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]
• Preparation and Storage: Store all reagents at 2-8 degree C

Related Product Information for Rock2 elisa kit

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only! It is intended to be determinated Rock2 concentrations in Human serum, plasma and other body fluids. Using Purified Human Rock2 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add Rock2 and Rock2 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Rock2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 269849761
• NCBI GeneID: 9475
• NCBI Official Full Name: Rho-associated protein kinase 2
• NCBI Official Synonym Full Names: Rho-associated, coiled-coil containing protein kinase 2
• NCBI Official Symbol: ROCK2
• NCBI Official Synonym Symbols: ROCK-II
• NCBI Protein Information: rho-associated protein kinase 2; p164 ROCK-2; rho-associated, coiled-coil-containing protein kinase II
• UniProt Protein Name: Rho-associated protein kinase 2
• Protein Family: Rho-associated protein kinase
• UniProt Gene Name: ROCK2
• UniProt Synonym Gene Names: KIAA0619; ROCK-II

NCBI Description

The protein encoded by this gene is a serine/threonine kinase that regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element. This protein, which is an isozyme of ROCK1 is a target for the small GTPase Rho. [provided by RefSeq, Jul 2008]

Uniprot Description

ROCK2: Protein kinase which is a key regulator of actin cytoskeleton and cell polarity. Involved in regulation of smooth muscle contraction, actin cytoskeleton organization, stress fiber and focal adhesion formation, neurite retraction, cell adhesion and motility via phosphorylation of ADD1, BRCA2, CNN1, EZR, DPYSL2, EP300, MSN, MYL9/MLC2, NPM1, RDX, PPP1R12A and VIM. Phosphorylates SORL1 and IRF4. Acts as a negative regulator of VEGF-induced angiogenic endothelial cell activation. Positively regulates the activation of p42/MAPK1-p44/MAPK3 and of p90RSK/RPS6KA1 during myogenic differentiation. Plays an important role in the timely initiation of centrosome duplication. Inhibits keratinocyte terminal differentiation. May regulate closure of the eyelids and ventral body wall through organization of actomyosin bundles. Plays a critical role in the regulation of spine and synaptic properties in the hippocampus. Homodimer. Interacts with IRS1, RHOB and RHOC. Interacts with RHOA (activated by GTP), PPP1R12A, CHORDC1, SORL1, EP300 and BRCA2. Interacts with NPM1 and this interaction enhances its activity. Interacts with RAF1. Activated by RHOA binding. Inhibited by Y-27632. Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.

Protein type: Protein kinase, Ser/Thr (non-receptor); Protein kinase, AGC; Kinase, protein; EC 2.7.11.1; AGC group; DMPK family; ROCK subfamily

Chromosomal Location of Human Ortholog: 2p24

Cellular Component: centrosome; plasma membrane; spindle pole centrosome; cytosol; nucleus

Molecular Function: protein serine/threonine kinase activity; protein binding; Rho GTPase binding; metal ion binding; structural molecule activity; ATP binding

Biological Process: regulation of cell adhesion; axon guidance; dendrite morphogenesis; rhythmic process; cytokinesis; regulation of circadian rhythm; protein amino acid phosphorylation; Rho protein signal transduction; negative regulation of angiogenesis; smooth muscle contraction; induction of apoptosis via death domain receptors; regulation of stress fiber formation; regulation of actin cytoskeleton organization and biogenesis; neural tube closure; regulation of focal adhesion formation; ephrin receptor signaling pathway; vascular endothelial growth factor receptor signaling pathway; actin cytoskeleton organization and biogenesis; centrosome duplication; regulation of keratinocyte differentiation