Human Rho-associated, coiled-coil containing protein kinase 1 ELISA Kit | ROCK1 elisa kit

Human Rho-associated protein kinase 1, ROCK1 ELISA Kit

Cat. Num. AAA9318720

• Gene Names: ROCK1; ROCK-I; P160ROCK
• Reactivity: Human
• Synonyms: Rho-associated; coiled-coil containing protein kinase 1; Human Rho-associated protein kinase 1; ROCK1 ELISA Kit; Human Rho-associated protein kinase 1 (ROCK1) ELISA kit; MGC131603; MGC43611; P160ROCK; PRO0435; Rho kinase; p160-ROCK; renal carcinoma antigen NY-REN-35; ROCK1 elisa kit

• Reactivity: Human
• Specificity: No significant cross-reactivity or interference between Human ROCK1 and analogues was observed.

• Samples: Serum, Plasma, Tissue Homogenate, Feces and Urine
• Assay Type: Sandwich
• Detection Range: 0.25ng/ml-8ng/ml.
• Sensitivity: 0.1ng/ml.
• Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic applications! It is intended to be determinated ROCK1 concentrations in Human serum, plasma and other body fluids. Using Purified Human ROCK1 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add ROCK1 and ROCK1 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of ROCK1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Intra-assay Precision: Intra-assay CV (%) is less than 15%.
• Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]
• Preparation and Storage: Store all reagents at 2-8 degree C

Related Product Information for ROCK1 elisa kit

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only! It is intended to be determinated ROCK1 concentrations in Human serum, plasma and other body fluids. Using Purified Human ROCK1 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add ROCK1 and ROCK1 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of ROCK1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4885583
• NCBI GeneID: 6093
• NCBI Accession #: NP_005397.1
• NCBI GenBank Nucleotide #: NM_005406.2
• UniProt Accession #: Q13464; Q2KHM4; Q59GZ4; B0YJ91
• Molecular Weight: 158,175 Da
• NCBI Official Full Name: rho-associated protein kinase 1
• NCBI Official Synonym Full Names: Rho-associated, coiled-coil containing protein kinase 1
• NCBI Official Symbol: ROCK1
• NCBI Official Synonym Symbols: ROCK-I; P160ROCK
• NCBI Protein Information: rho-associated protein kinase 1; p160-ROCK; Rho kinase; p160 ROCK-1; renal carcinoma antigen NY-REN-35; rho-associated, coiled-coil-containing protein kinase 1; rho-associated, coiled-coil-containing protein kinase I
• UniProt Protein Name: Rho-associated protein kinase 1
• Protein Family: Rho-associated protein kinase
• UniProt Gene Name: ROCK1
• UniProt Synonym Gene Names: ROCK-I; p160ROCK
• UniProt Entry Name: ROCK1_HUMAN

NCBI Description

This gene encodes a protein serine/threonine kinase that is activated when bound to the GTP-bound form of Rho. The small GTPase Rho regulates formation of focal adhesions and stress fibers of fibroblasts, as well as adhesion and aggregation of platelets and lymphocytes by shuttling between the inactive GDP-bound form and the active GTP-bound form. Rho is also essential in cytokinesis and plays a role in transcriptional activation by serum response factor. This protein, a downstream effector of Rho, phosphorylates and activates LIM kinase, which in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. [provided by RefSeq, Jul 2008]

Uniprot Description

ROCK1: Protein kinase which is a key regulator of actin cytoskeleton and cell polarity. Involved in regulation of smooth muscle contraction, actin cytoskeleton organization, stress fiber and focal adhesion formation, neurite retraction, cell adhesion and motility via phosphorylation of DAPK3, GFAP, LIMK1, LIMK2, MYL9/MLC2, PFN1 and PPP1R12A. Phosphorylates FHOD1 and acts synergistically with it to promote SRC-dependent non-apoptotic plasma membrane blebbing. Phosphorylates JIP3 and regulates the recruitment of JNK to JIP3 upon UVB-induced stress. Acts as a suppressor of inflammatory cell migration by regulating PTEN phosphorylation and stability. Acts as a negative regulator of VEGF-induced angiogenic endothelial cell activation. Required for centrosome positioning and centrosome-dependent exit from mitosis. Plays a role in terminal erythroid differentiation. May regulate closure of the eyelids and ventral body wall by inducing the assembly of actomyosin bundles. Promotes keratinocyte terminal differentiation. Homodimer. Interacts with RHOB, RHOC, MYLC2B snd PTEN. Interacts with RHOA (activated by GTP), CHORDC1, DAPK3, GEM, JIP3, RHOE, PPP1R12A, PFN1, LIMK1, LIMK2 and TSG101. Interacts with FHOD1 in a Src-dependent manner. Detected in blood platelets. Activated by RHOA binding. Inhibited by Y- 27632. Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.

Protein type: Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; Protein kinase, AGC; Kinase, protein; AGC group; DMPK family; ROCK subfamily

Chromosomal Location of Human Ortholog: 18q11.1

Cellular Component: Golgi membrane; centriole; ruffle; cytoskeleton; lamellipodium; plasma membrane; cytosol

Molecular Function: protein serine/threonine kinase activity; protein binding; metal ion binding; GTP-Rho binding; ATP binding; protein kinase activity

Biological Process: regulation of cell adhesion; axon guidance; apoptosis; bleb formation; signal transduction; protein amino acid phosphorylation; Rho protein signal transduction; leukocyte adhesion; negative regulation of angiogenesis; smooth muscle contraction; positive regulation of focal adhesion formation; regulation of actin cytoskeleton organization and biogenesis; regulation of stress fiber formation; myoblast migration; regulation of focal adhesion formation; membrane to membrane docking; ephrin receptor signaling pathway; negative regulation of neuron apoptosis; vascular endothelial growth factor receptor signaling pathway; leukocyte tethering or rolling; actin cytoskeleton organization and biogenesis; regulation of keratinocyte differentiation; leukocyte migration; cell structure disassembly during apoptosis