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Typical Testing Data/Standard Curve (for reference only)

Human roundabout homolog 4, magic roundabout (Drosophila) ELISA Kit | ROBO4 elisa kit

Human Roundabout homolog 4, ROBO4 ELISA Kit

Gene Names
ROBO4; MRB; ECSM4
Reactivity
Human
Synonyms
roundabout homolog 4; magic roundabout (Drosophila); Human Roundabout homolog 4; ROBO4 ELISA Kit; Human Roundabout homolog 4 (ROBO4) ELISA kit; ECSM4; FLJ20798; MGC133352; MGC133353; MRB; magic roundabout; ROBO4 elisa kit
Ordering
For Research Use Only!
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of mouse RNASE2. No significant cross-reactivity or interference between mouse RNASE2 and analogues was observed.
Sequence Length
1007
Samples
Serum, Plasma, Tissue Homogenates
Assay Type
Quantitative Sandwich
Detection Range
3.12 ng/ml-200 ng/ml
Sensitivity
0.78 ng/ml.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Typical Testing Data/Standard Curve (for reference only)
Related Product Information for ROBO4 elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for RNASE2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any RNASE2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RNASE2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RNASE2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
107,457 Da
NCBI Official Full Name
roundabout homolog 4 isoform 1
NCBI Official Synonym Full Names
roundabout, axon guidance receptor, homolog 4 (Drosophila)
NCBI Official Symbol
ROBO4
NCBI Official Synonym Symbols
MRB; ECSM4
NCBI Protein Information
roundabout homolog 4; roundabout homolog 4, magic roundabout
UniProt Protein Name
Roundabout homolog 4
Protein Family
UniProt Gene Name
ROBO4
UniProt Synonym Gene Names
UNQ421/PRO3674
UniProt Entry Name
ROBO4_HUMAN

Uniprot Description

ROBO4: Receptor for Slit proteins, at least for SLIT2, and seems to be involved in angiogenesis and vascular patterning. May mediate the inhibition of primary endothelial cell migration by Slit proteins. Belongs to the immunoglobulin superfamily. ROBO family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 11q24.2

Cellular Component: integral to membrane; external side of plasma membrane

Molecular Function: receptor activity

Biological Process: angiogenesis; cell differentiation; regulation of cell migration; negative regulation of cell migration

Research Articles on ROBO4

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Product Notes

The Human ROBO4 robo4 (Catalog #AAA946210) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA946210 ELISA Kit recognizes Human ROBO4. It is sometimes possible for the material contained within the vial of "roundabout homolog 4, magic roundabout (Drosophila), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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