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Typical Testing Data

Human Ribonuclease inhibitor ELISA Kit | RNH1 elisa kit

Human Ribonuclease inhibitor ELISA Kit

Gene Names
RNH1; RAI; RNH
Reactivity
Human
Synonyms
Ribonuclease inhibitor; Human Ribonuclease inhibitor ELISA Kit; RNH1/RAI/RNH/PRI/Placental RNase inhibitor/Placental ribonuclease inhibitor/Ribonuclease; angiogenin inhibitor 1; RNH1 elisa kit
Ordering
For Research Use Only!
Reactivity
Human
Sequence Length
461
Samples
Serum, plasma and other biological fluids.
Assay Type
Sandwich ELISA, Double Antibody
Detection Range
0.313-20pg/ml
Sensitivity
0.938ng/ml
Preparation and Storage
Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Testing Data

Typical Standard Curve

Typical Standard Curve
Related Product Information for RNH1 elisa kit
Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human RNH1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human RNH1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRPStreptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human RNH1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human RNH1 can be calculated.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
49,973 Da
NCBI Official Full Name
ribonuclease inhibitor
NCBI Official Synonym Full Names
ribonuclease/angiogenin inhibitor 1
NCBI Official Symbol
RNH1
NCBI Official Synonym Symbols
RAI; RNH
NCBI Protein Information
ribonuclease inhibitor
UniProt Protein Name
Ribonuclease inhibitor
Protein Family
UniProt Gene Name
RNH1
UniProt Synonym Gene Names
PRI; RNH; Placental RNase inhibitor; RAI
UniProt Entry Name
RINI_HUMAN

NCBI Description

Placental ribonuclease inhibitor (PRI) is a member of a family of proteinaceous cytoplasmic RNase inhibitors that occur in many tissues and bind to both intracellular and extracellular RNases (summarized by Lee et al., 1988 [PubMed 3219362]). In addition to control of intracellular RNases, the inhibitor may have a role in the regulation of angiogenin (MIM 105850). Ribonuclease inhibitor, of 50,000 Da, binds to ribonucleases and holds them in a latent form. Since neutral and alkaline ribonucleases probably play a critical role in the turnover of RNA in eukaryotic cells, RNH may be essential for control of mRNA turnover; the interaction of eukaryotic cells with ribonuclease may be reversible in vivo.[supplied by OMIM, Jul 2010]

Uniprot Description

RNH1: Ribonuclease inhibitor which inhibits RNASE1, RNASE2 and ANG. May play a role in redox homeostasis.

Protein type: Inhibitor

Chromosomal Location of Human Ortholog: 11p15.5

Cellular Component: angiogenin-PRI complex; cytoplasm; nucleoplasm

Molecular Function: protein binding; ribonuclease inhibitor activity

Biological Process: mRNA catabolic process; negative regulation of catalytic activity; regulation of angiogenesis

Research Articles on RNH1

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Product Notes

The Human RNH1 rnh1 (Catalog #AAA760274) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA760274 ELISA Kit recognizes Human RNH1. It is sometimes possible for the material contained within the vial of "Ribonuclease inhibitor, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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