INFLUENZA B RNA Control

INFLUENZA B RNA CONTROL

• Synonyms: INFLUENZA B; INFLUENZA B RNA CONTROL; INFLUENZA B rna control

• Concentration: copies/ul 13000 (varies by lot)

• Characterstic: The lyophilized nucleic acid is included in a thermo-sealed foil pouch containing a silica gel bag. It is necessary to reconstitute it before use (Refer to "Preparation of Reagents").
• Preparation: Grown in MOCK infected cells.
• Extract Preparation: Commercial genomic RNA extraction method.
• Kit Contents: [1] INB RNA CONTROL : 1 vial with lyophilized RNA of influenza B virus, (B/Brisbane/60/2008), (12500-20000 copies/ul once reconstituted). RNA quantification has been performed by real-time PCR.; [2] CONTROL RECONSTITUTION SOLUTION : 500 ul of molecular biology grade water, DNase, RNase free.
• Recommendations and Precautions: 1. For research use only. ; 2. Sterile tips with aerosol barrier are essential to prevent contamination.; 3. Specimens should be hand led as in the case of infectious samples using research sa fety laboratory procedures.; Thoroughly clean and disinfect all work surfaces with a freshly prepared solution of 0.5% sodium hypochlorite in deionized or distilled water.; 4. In order to perform the test it is essential to have separate working areas.; 5. Dispose of unused reagents and waste in accordance with all applicable regulations.; 6. The component RNA CONTROL could include genetic material or substances of animal and/or human origin. RNA CONTROL could contain in activated antigen. RNA CONTROL contains purified nucleic acids obtained from inactivated microorganism, nevert heless, it should be considered potentially infectious and handled with care. No present method can offer complete assurance that these or other infectious agents are absent. All materials should be handled and disposed as of potentially infectious. Observe the local regulations for waste disposal.; 7. Dilutions below 1000 copies/ul should be made immediately before use. Freezing of product dilutions containing less than 1000 copies/ul is not recommended as partial RNA degradation might occur.
• Stability and Handling of reagents: Handle reagents in aseptic conditions to avoid microbial contaminations.; Use only the amount or reagent required for the test.; After control resuspension RNA solution should be aliquoted in order to avoid multiple freeze-thaw cycles. The product is stable until the expiry date indicated in the label, if the instructions for use are followed.
• Preparation of the Reagents: 1. Open the zip lock bag containing RNA CONTROL [1] and remove the foil wrapped around the vial.; 2. Centrifuge RNA CONTROL [1] 1 minute at 1000 g.; 3. Add 50ul of CONTROL RECONSTITUTION SOLUTION [2] and mix until completely reconstituted. The concentration will be 12500-20000 copies/ul once reconstituted.; 4. Shake with vortex for 30 seconds to dissolve and homogenize completely.
• Internal Quality Control: Each batch is subjected to internal quality control testing before re leasing. Quality control analys is is performed by realtime PCR. Final quality control results for each particular lot are available.
• Note: Purified RNA of influenza B H3 virus to be used to control techniques based in nucleic acids amplification.
• Preparation and Storage: Special transport conditions not required. Store the lyophilized vial at 2-8°C inside the zip lock bag. Once the zip lock back is opened, reconstitute the lyophilized vial immediately and store -70°C to -90°C after reconstitution (temperature indicated on the label).

Product Categories/Family for INFLUENZA B rna control

DNA/RNA Amplification Controls

NCBI and Uniprot Product Information