Riboflavin transporter RibU (ribU) Recombinant Protein | LLNZ_06150 recombinant protein
Recombinant Lactococcus lactis subsp. cremoris Riboflavin transporter RibU (ribU)
NCBI and Uniprot Product Information
Uniprot Description
Function: Probably a riboflavin-binding protein that interacts with the energy-coupling factor (ECF) ABC-transporter complex. Unlike classic ABC transporters this ECF transporter provides the energy necessary to transport a number of different substrates. The substrates themselves are bound by transmembrane, not extracytoplasmic soluble proteins
By similarity. Mediates riboflavin uptake, probably also transports FMN and roseoflavin. Ref.1
Enzyme regulation: Inhibited by FMN and roseoflavin. Ref.1
Subunit structure: Forms a stable energy-coupling factor (ECF) transporter complex composed of a membrane-embedded substrate-binding protein (S component), two ATP-binding proteins (A and A' components) and a transmembrane protein (T component)
By similarity.
Subcellular location: Cell membrane; Multi-pass membrane protein Ref.3.
Induction: Induced by riboflavin deficiency. Ref.1
Domain: Riboflavin is stacked with one or more Trp residues in the binding pocket of RibU. Ref.3
Disruption phenotype: Mutant exhibits altered transcriptional control of the riboflavin biosynthesis operon in response to riboflavin and FMN, and does not consume riboflavin from the growth medium. Ref.1
Miscellaneous: Riboflavin, FMN and roseoflavin, but not FAD, bind to RibU with high affinity and 1:1 stoichiometry.
Sequence similarities: Belongs to the prokaryotic riboflavin transporter (P-RFT) (TC 2.A.87) family. [View classification]
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Product Notes
The LLNZ_06150 ribu (Catalog #AAA1256302) is a Recombinant Protein produced from E Coli or Yeast or Baculovirus or Mammalian Cell and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 1-206. The amino acid sequence is listed below: MSKTRRMVLI AMLAALSTIL LLPILQFPLL PGIDFMKVEL SIIPVLIGVF TLGLGDGFII LFIRSVLWYL LFNQGPSTWI GVPMNFVALG IFMAIVWFFT KKKFSIKNYT VGIVLATIAS VLVMMVLNVF YALPLYRLAA GFDVDKIFAG ATHLFNMGSL SVTLNPTYLL TVVLPFNALQ YIIFALVFGL IVTVFKKNKV VKFYNA. It is sometimes possible for the material contained within the vial of "Riboflavin transporter RibU (ribU), Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
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