Human regulator of G-protein signaling 3 ELISA Kit | RGS3 elisa kit

Human Regulator of G-protein signaling 3, RGS3 ELISA Kit

Cat. Num. AAA9334667

• Gene Names: RGS3; C2PA; RGP3
• Reactivity: Human
• Synonyms: regulator of G-protein signaling 3; Human Regulator of G-protein signaling 3; RGS3 ELISA Kit; Human Regulator of G-protein signaling 3 (RGS3) ELISA kit; C2PA; FLJ20370; FLJ31516; FLJ90496; PDZ-RGS3; RGP3; regulator of G-protein signalling 3; RGS3 elisa kit

• Reactivity: Human
• Specificity: No significant cross-reactivity or interference between Human RGS3 and analogues was observed.

• Samples: Serum, Plasma, Tissue Homogenate, Feces and Urine
• Assay Type: Sandwich
• Detection Range: 31.2 pg/ml - 1000 pg/ml.
• Sensitivity: 5.0 pg/ml.
• Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic applications! It is intended to be determinated RGS3 concentrations in Human serum, plasma and other body fluids. Using Purified Human RGS3 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add RGS3 and RGS3 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of RGS3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Intra-assay Precision: Intra-assay CV (%) is less than 15%.
• Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]
• Preparation and Storage: Store all reagents at 2-8 degree C

Related Product Information for RGS3 elisa kit

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only! It is intended to be determinated RGS3 concentrations in Human serum, plasma and other body fluids. Using Purified Human RGS3 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add RGS3 and RGS3 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of RGS3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 442654297
• NCBI GeneID: 5998
• NCBI Accession #: NP_001263189.1
• NCBI GenBank Nucleotide #: NM_001276260.1
• UniProt Accession #: P49796; Q5VXB8; Q5VXC1; Q5VZ05; Q5VZ06; Q6ZRM5; Q8IUQ1; Q8NC47; Q8NFN4; A6NHA0; A8K0V1; B3KUB2
• Molecular Weight: 132,336 Da
• NCBI Official Full Name: regulator of G-protein signaling 3 isoform 2
• NCBI Official Synonym Full Names: regulator of G-protein signaling 3
• NCBI Official Symbol: RGS3
• NCBI Official Synonym Symbols: C2PA; RGP3
• NCBI Protein Information: regulator of G-protein signaling 3; regulator of G-protein signalling 3
• UniProt Protein Name: Regulator of G-protein signaling 3
• Protein Family: Regulator of G-protein signaling
• UniProt Gene Name: RGS3
• UniProt Synonym Gene Names: RGP3; RGS3
• UniProt Entry Name: RGS3_HUMAN

NCBI Description

This gene encodes a member of the regulator of G-protein signaling (RGS) family. This protein is a GTPase-activating protein that inhibits G-protein-mediated signal transduction. Alternative splicing and the use of alternative promoters results in multiple transcript variants encoding different isoforms. Long isoforms are largely cytosolic and plasma membrane-associated with a function in Wnt signaling and in the epithelial mesenchymal transition, while shorter N-terminally-truncated isoforms can be nuclear. [provided by RefSeq, Jan 2013]

Uniprot Description

RGS3: Down-regulates signaling from heterotrimeric G-proteins by increasing the GTPase activity of the alpha subunits, thereby driving them into their inactive GDP-bound form. Down-regulates G- protein-mediated release of inositol phosphates and activation of MAP kinases. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: GAPs, RGS; GAPs

Chromosomal Location of Human Ortholog: 9q32

Cellular Component: nucleoplasm; cytoplasm; plasma membrane; cytosol

Molecular Function: GTPase activator activity

Biological Process: regulation of G-protein coupled receptor protein signaling pathway; positive regulation of GTPase activity; inactivation of MAPK activity