Bpu10I Restriction Enzyme

Bpu10I

Cat. Num. AAA638441

• Purity: Molecular Biology Grade
• Synonyms: Bpu10I; Bpu10I restriction enzyme

• Purity/Purification: Molecular Biology Grade

• Dilution Buffer: 10mM Tris-HCl (pH 7.4 at 25 degree C), 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA and 50% glycerol. For longer periods-the Storage Buffer should be used.
• Compatible Ends: Bpu1102I, DdeI, Eco81I.
• pACYC177: 5
• pACYC184: 3
• Labeled Oligonucleotide (LO) Assay: No detectable degradation of a single-stranded and double-stranded labeled oligonucleotide was observed after incubation with 10units of restriction endonuclease for 4 hours.
• Lambda: 19
• Ligation/Recutting Assay: After 50-fold overdigestion (3u/ug DNA x 17 hours) with Bpu10I, more than 90% of the pBR322 DNA fragments can be ligated at a 5'-termini concentration of 0.1uM. More than 90% of these can be recut. Where there is more than one Bpu10I recognition site in the substrate DNA, DNA fragments, obtained after cleavage with Bpu10I (due to its asymmetric recognition sequence), may join each other in 3 different ways and in only one case is the resulting sequence recognized by Bpu10I. Therefore, restriction after ligation yields no more than 30% efficiency. Other two sequences resulting from ligation of Bpu10I DNA fragments may be cleaved with Eco81I (SauI) and Bpu1102I (EspI)
• M13mp18/19: 4
• Note: A large excess of enzyme (>4u/1ug DNA) may result in incomplete DNA cleavage. Therefore, we recommend increasing the incubation time instead of using an excess of Bpu10I.
• Overdigestion Assay: No detectable change in the specific fragmentation pattern is observed after 48-fold overdigestion (3u/ug lambda DNA x 16 hours) with Bpu10I (see Star Activity).
• pBR322: 1
• PhiX174: 7
• Stability During Prolonged Incubation: A minimum of 0.2units of enzyme is required for complete digestion of 1ug of lambda DNA in 16 hours at 37 degree C.
• Storage Buffer: 10mM Tris-HCl (pH 7.5 at 25 degree C), 200mM KCl, 1mM DTT, 0.1mM EDTA, 0.2mg/ml BSA and 50% glycerol.
• Thermal Inactivation: Enzyme is inactivated by incubation at 80 degree C for 20min.
• Unit Definition: One unit is defined as the amount of enzyme required to digest 1ug of lambda DNA fragments in 1 hour at 37 degree C in 50ul of assay buffer.
• Preparation and Storage: -20 degree C

Testing Data

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Related Product Information for Bpu10I restriction enzyme

5'-C C^T N A G C-3'

Product Categories/Family for Bpu10I restriction enzyme

Molecular Biology; MB-Enzymes; Restriction

NCBI and Uniprot Product Information

• NCBI GI #: 395318922
• NCBI Official Full Name: Bpu10I restriction endonuclease