Rat RE1-silencing transcription factor ELISA Kit | REST elisa kit

Rat RE1-silencing transcription factor, REST ELISA Kit

Cat. Num. AAA9332070

• Reactivity: Rat
• Synonyms: RE1-silencing transcription factor; Rat RE1-silencing transcription factor; REST ELISA Kit; Rat RE1-silencing transcription factor (REST) ELISA kit; NRSF; XBR; neuron restrictive silencer factor; repressor binding to the X2 box; REST elisa kit

• Reactivity: Rat

• Assay Type: Quantitative Sandwich
• Detection Range: 62.5pg/ml-2000pg/ml
• Sensitivity: 10pg/ml
• Intra-assay Precision: Intra-assay CV (%) is less than 15%.
• Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]. All CV% should be compared by concentration, not compared by OD values.
• Preparation and Storage: Store all reagents at 2-8 degree C

Related Product Information for REST elisa kit

Background/Introduction: This Quantitative Sandwich ELISA kit is for lab reagent/research use only, not for drug, household, therapeutic or test applications! This kit is intended to be used for determine the level of REST (hereafter termed "analyte") in undiluted original Rat serum, plasma or tissue homogenates samples. For other sample types please contact tech support to determine compatibility with this assay. This kit is not suitable for assaying non-biological sources of substances.

NCBI and Uniprot Product Information

• NCBI GI #: 13929116
• NCBI GeneID: 83618
• NCBI Accession #: NP_113976.1
• NCBI GenBank Nucleotide #: NM_031788.1
• UniProt Accession #: O54963; O54964
• Molecular Weight: 117,126 Da
• NCBI Official Full Name: RE1-silencing transcription factor
• NCBI Official Synonym Full Names: RE1-silencing transcription factor
• NCBI Official Symbol: Rest
• NCBI Protein Information: RE1-silencing transcription factor; neural-restrictive silencer factor; zinc finger transcription factor REST protein
• UniProt Protein Name: RE1-silencing transcription factor
• Protein Family: RE1-silencing transcription factor
• UniProt Gene Name: Rest
• UniProt Synonym Gene Names: Nrsf
• UniProt Entry Name: REST_RAT

NCBI Description

represses transcription of the AMPA receptor (AMPAR) subunit GluR2 and other neural genes [RGD, Feb 2006]

Uniprot Description

REST: a transcriptional repressor that binds neuron-restrictive silencer element (NRSE) and represses neuronal gene transcription in non-neuronal cells during embryonic development. Induced during ageing in human cortical and hippocampal neurons, where it represses genes that promote cell death and induces the expression of stress response genes. Protects neurons from amyloid beta- and oxidative stress-induced toxicity. The deletion of REST in the mouse brain induces age-related neurodegeneration. Restricts the expression of neuronal genes by associating with two distinct corepressors, SIN3A and RCOR1, which in turn recruit histone deacetylase to the promoters of REST-regulated genes. Mediates repression by recruiting the BHC complex at NRSE sites which acts by deacetylating and demethylating specific sites on histones, thereby acting as a chromatin modifier. REST activates DYRK1A transcription via a neuron-restricted silencer element in the DYRK1A promoter. Four isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription factor; DNA-binding; C2H2-type zinc finger protein; Cell development/differentiation

Cellular Component: protein complex; transcriptional repressor complex; chromatin; cytosol; nucleus

Molecular Function: protein binding; nucleic acid binding; DNA binding; metal ion binding; outward rectifier potassium channel activity; protein complex binding; chromatin binding; transcription factor activity; transcription factor binding

Biological Process: transcription from RNA polymerase II promoter; regulation of transcription from RNA polymerase II promoter; negative regulation of cell proliferation; negative regulation of neuron differentiation; negative regulation of calcium ion-dependent exocytosis; positive regulation of apoptosis; positive regulation of transcription, DNA-dependent; negative regulation of aldosterone biosynthetic process; positive regulation of caspase activity; positive regulation of transcription from RNA polymerase II promoter; hemopoietic progenitor cell differentiation; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; negative regulation of neurogenesis; negative regulation of insulin secretion