CLEC4G active protein

CLEC4G, Recombinant, Human (C-type Lectin Domain Family 4 Member G, Liver and Lymph Node Sinusoidal Endothelial Cell C-type Lectin, LSECtin)

Cat. Num. AAA637678

• Gene Names: CLEC4G; LP2698; UNQ431; DTTR431; LSECtin
• Purity: Highly Purified; 90%, as determined by SDS-PAGE under reducing conditions and visualized by silver stain.
• Synonyms: CLEC4G; Recombinant; Human (C-type Lectin Domain Family 4 Member G; Liver and Lymph Node Sinusoidal Endothelial Cell C-type Lectin; LSECtin); CLEC4G active protein

• Host: Recombinant Human
• Purity/Purification: Highly Purified; 90%, as determined by SDS-PAGE under reducing conditions and visualized by silver stain.
• Form/Format: Supplied as a lyophilized powder in 50mM Tris-citrate, 0.3M sodium chloride, pH 6.0.. Reconstitute with PBS to 100ug/ml.

• Activity: Measured by its ability to bind Jurkat cells.
• Endotoxin Level: 1EU/1ug.
• Preparation and Storage: Lyophilized powder may be stored at 4 degree C for short-term only. Reconstitute to nominal volume by adding sterile PBS and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for CLEC4G active protein

LSECtin (liver and lymph node sinusoidal endothelial cell C-type lectin), also known as C-type lectin superfamily 4, member G (CLEC4G), is a member of subgroup II of the C-type (Ca2+-dependent) lectin superfamily (1). The protein was named LSECtin because its initial expression was described to be restricted to liver and lymph node sinusoidal endothelial cells (1). Since then, however, LSECtin has also been detected in peripheral blood and thymic dendritic cells isolated ex vivo, and in monocyte-derived macrophages and dendritic cells at the RNA and protein level (2). Human LSECtin is an approximately 40kD, single-pass, type II transmembrane glycoprotein that is 293 amino acids (aa) in length. It contains a short N-terminal cytoplasmic tail (aa 1-31) and a 21 aa transmembrane region. Its extracellular region consists of two N-linked glycosylation sites (aa 73 and 159), a coil-coil neck domain (aa 96-136), a C-type lectin-like domain (CTLD) of the type found in human DC-SIGN and DC-SIGN receptor (aa 165-289), and a C-terminal Ca2+-dependent carbohydrate-recognition domain (C-type CRD) (1). Human LSECtin shares 64% aa sequence identity with mouse LSECtin. LSECtin binds to mannose, GlcNAc, and fucose in a Ca2+-dependent manner (1-3). In addition, LSECtin has the ability to bind to surface glycoproteins of enveloped viruses (3, 4). In particular, interaction of LSECtin with the surface glycoproteins of severe acute respiratory syndrome (SARS) coronavirus and Ebola virus has been described, and LSECtin-mediated infection of cells by Ebola virus has been demonstrated (3, 4).

A DNA sequence encoding the extracellular domain of human LSECtin (Ser 54-Cys 293; Accession # NP_940894) was fused to a nine-histidine tag at the N-terminus. The protein was expressed in a mouse myeloma cell line, NS0.
Calculated molecular mass of 27.8kD. As a result of glycosylation, the recombinant protein migrates as an ~ 37kD protein in SDS-PAGE under reducing conditions.

Product Categories/Family for CLEC4G active protein

Molecular Biology; MB-Receptors

NCBI and Uniprot Product Information

• NCBI GI #: 349732269
• NCBI GeneID: 339390
• NCBI Accession #: NP_001231785.1
• NCBI GenBank Nucleotide #: NM_001244856.1
• UniProt Accession #: Q6UXB4
• Molecular Weight: 32,562 Da
• NCBI Official Full Name: C-type lectin domain family 4 member G isoform 2
• NCBI Official Synonym Full Names: C-type lectin domain family 4, member G
• NCBI Official Symbol: CLEC4G
• NCBI Official Synonym Symbols: LP2698; UNQ431; DTTR431; LSECtin
• NCBI Protein Information: C-type lectin domain family 4 member G; C-type lectin superfamily 4, member G; liver and lymph node sinusoidal endothelial cell C-type lectin
• UniProt Protein Name: C-type lectin domain family 4 member G
• Protein Family: C-type lectin domain family
• UniProt Gene Name: CLEC4G
• UniProt Entry Name: CLC4G_HUMAN

NCBI Description

This gene encodes a glycan-binding receptor and member of the C-type lectin family which plays a role in the T-cell immune response. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]

Uniprot Description

CLEC4G: Binds mannose, N-acetylglucosamine (GlcNAc) and fucose, but not galactose, in a Ca(2+)-dependent manner, in vitro. {ECO:0000269|PubMed:14711836}

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 19p13.2

Cellular Component: integral to membrane

Molecular Function: protein binding; carbohydrate binding