Exodeoxyribonuclease V alpha chain (recD) Recombinant Protein | recD recombinant protein

Recombinant Escherichia coli Exodeoxyribonuclease V alpha chain (recD)

Cat. Num. AAA1100033

• Gene Names: recD; ECK2815; hopE; JW2787
• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: Exodeoxyribonuclease V alpha chain (recD); Recombinant Escherichia coli Exodeoxyribonuclease V alpha chain (recD); recD recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process)
• Sequence Positions: 1-608, full length protein
• Sequence: MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSHDAGEGHVCLPLSRLENNEASHPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMILCGDRLYLNRMWCNERTVARFFNEVNHAIEVDEALLAQTLDKLFPVSDEINWQKVAAAVALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGKALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDLPMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERARQLSRLTGTHVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTDIEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFGVAGLNERIEQFMQQKRKIHRHPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGTRVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELVYTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRE
• Sequence Length: 608

• Species: Escherichia coli (strain K12)
• Preparation and Storage: Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.

NCBI and Uniprot Product Information

• NCBI GI #: 16130723
• NCBI GeneID: 947287
• NCBI Accession #: NP_417296.1
• NCBI GenBank Nucleotide #: NC_000913.3
• UniProt Accession #: P04993; Q2MA18; Q59378
• Molecular Weight: 66,902 Da
• NCBI Official Full Name: exonuclease V (RecBCD complex), alpha chain
• NCBI Official Symbol: recD
• NCBI Official Synonym Symbols: ECK2815; hopE; JW2787
• NCBI Protein Information: exonuclease V (RecBCD complex), alpha chain
• UniProt Protein Name: RecBCD enzyme subunit RecD
• Protein Family: RecBCD enzyme
• UniProt Gene Name: recD
• UniProt Synonym Gene Names: ExoV subunit RecD

NCBI Description

RecBCD is a bipolar helicase. [More information is available at EcoGene: EG10826]. RecB, RecC, and RecD make up a helicase/nuclease complex that is essential for both homologous recombination during the course of transduction and conjugation as well as in repair of double-strand breaks in Escherichia coli . [More information is available at EcoCyc: EG10826].

Uniprot Description

A helicase/nuclease that prepares dsDNA breaks (DSB) for recombinational DNA repair. Binds to DSBs and unwinds DNA via a rapid (>1 kb/second) and highly processive (>30 kb) ATP-dependent bidirectional helicase. Unwinds dsDNA until it encounters a Chi (crossover hotspot instigator, 5'-GCTGGTGG-3') sequence from the 3' direction. Cuts ssDNA a few nucleotides 3' to Chi site, by nicking one strand or switching the strand degraded (depending on the reaction conditions). The properties and activities of the enzyme are changed at Chi. The Chi-altered holoenzyme produces a long 3'-ssDNA overhang which facilitates RecA-binding to the ssDNA for homologous DNA recombination and repair. In the holoenzyme this subunit contributes ssDNA-dependent ATPase and fast 5'-3' helicase activity. When added to pre-assembled RecBC greatly stimulates nuclease activity and augments holoenzyme processivity. Negatively regulates the RecA-loading ability of RecBCD.