Data
OnePCR Ultra supermix w fluorescent dye Reagent | OnePCR reagent
OnePCR Ultra Supermix w Fluorescent Dye
Synonyms
OnePCR Ultra supermix w fluorescent dye; OnePCR Ultra Supermix w Fluorescent Dye; OnePCR reagent
Application Notes
No post-staining processing of DNA required.
Direct loading onto the agarose gel for electrophoresis.
Sensitivity - High degree of sensitivity equivalent to ethidium bromide.
Speed - Benefit for quick set up of PCR reaction such as colony PCR when screening for positive clones.
Compatibility - Use blue light or UV to detect the signal.
Direct loading onto the agarose gel for electrophoresis.
Sensitivity - High degree of sensitivity equivalent to ethidium bromide.
Speed - Benefit for quick set up of PCR reaction such as colony PCR when screening for positive clones.
Compatibility - Use blue light or UV to detect the signal.
Preparation and Storage
Store at 4 degree C up to 6 months.
Store at RT up to 3 months.
Store at -20 degree C up to 1 year.
Shipping Temperature: 4 degree C
Store at RT up to 3 months.
Store at -20 degree C up to 1 year.
Shipping Temperature: 4 degree C
Data
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Related Product Information for OnePCR reagent
Description:
OnePCR Ultra is a ready-to-use PCR reaction mixture. Simply add primers, template, and water, the reagent will execute primer extensions and other molecular biology applications. OnePCR Ultra is a pre-mixed solution containing Taq DNA polymerase, PCR buffer, dNTP, gel loading dyes, enhancer, and fluorescence dye. OnePCR Ultra which contains the Taq DNA polymerase is purified from the E. coli. and expressing the Thermus aquaticus DNA polymerase gene. This enzyme has a 5? --> 3? DNA polymerase and the 5? --> 3? exonuclease activity but lacks the 3? --> 5? exonuclease activity. OnePCR Ultra, which contains the fluorescence dye, is directly detected on LED transilluminator or UV epi-illuminator after the DNA electrophoresis. OnePCR Ultra mixture is supplied at the 2X concentration to allow approximately 50% of the final reaction volume to be used for the addition of primer and template solutions. Reagents are provided with the sufficient amplification reactions of 50 ul each.
OnePCR Ultra is a ready-to-use PCR reaction mixture. Simply add primers, template, and water, the reagent will execute primer extensions and other molecular biology applications. OnePCR Ultra is a pre-mixed solution containing Taq DNA polymerase, PCR buffer, dNTP, gel loading dyes, enhancer, and fluorescence dye. OnePCR Ultra which contains the Taq DNA polymerase is purified from the E. coli. and expressing the Thermus aquaticus DNA polymerase gene. This enzyme has a 5? --> 3? DNA polymerase and the 5? --> 3? exonuclease activity but lacks the 3? --> 5? exonuclease activity. OnePCR Ultra, which contains the fluorescence dye, is directly detected on LED transilluminator or UV epi-illuminator after the DNA electrophoresis. OnePCR Ultra mixture is supplied at the 2X concentration to allow approximately 50% of the final reaction volume to be used for the addition of primer and template solutions. Reagents are provided with the sufficient amplification reactions of 50 ul each.
Product Categories/Family for OnePCR reagent
Similar Products
Product Notes
The OnePCR (Catalog #AAA402026) is a Reagent and is intended for research purposes only. The product is available for immediate purchase. No post-staining processing of DNA required. Direct loading onto the agarose gel for electrophoresis. Sensitivity - High degree of sensitivity equivalent to ethidium bromide. Speed - Benefit for quick set up of PCR reaction such as colony PCR when screening for positive clones. Compatibility - Use blue light or UV to detect the signal. Researchers should empirically determine the suitability of the OnePCR for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "OnePCR Ultra supermix w fluorescent dye, Reagent" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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