Dulbecco's MEM (DMEM) w/o Amino Acids, Glucose Reagent
Dulbecco's MEM (DMEM) w/o Amino Acids, Glucose (Powder)
Synonyms
Dulbecco's MEM (DMEM) w/o Amino Acids; Glucose; Glucose (Powder); Glucose reagent
Appearance
Orange, homogenenous, free-flowing powder.
Solubility
Yellow, clear, complete
pH
4.9
Endotoxin
< 1 EU/ml
Directions per Liter
Dissolve 7.43g in 800-900ml of ddH2O, stirring gently until completely solubilized. If required, add 3.7g sodium bicarbonate with stirring. Adjust pH of the medium to 0.1-0.3 pH unit below the desired level. Add additional water to bring the solution to 1L. Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.
Components shown as g/liter
Inorganic Salts
Calcium Chloride•2H2O: 0.265 g/Liter
Ferric Nitrate 9H2O: 0.0001 g/Liter
Magnesium Sulfate: 0.09767 g/Liter
Potassium Chloride: 0.4 g/Liter
Sodium Chloride: 6.4 g/Liter
Sodium Phosphate Monobasic: 0.109 g/Liter
Amino Acids
L-Arginine HCl: Absent
L-Cystine 2 HCl: Absent
L-Glutamine: Absent
Glycine: Absent
L-Histidine HCl H2O: Absent
L-Isoleucine: Absent
L-Leucine: Absent
L-Lysine HCl: Absent
L-Methionine: Absent
L-Phenylalanine: Absent
L-Serine: Absent
L-Threonine: Absent
L-Tryptophan: Absent
L-Tyrosine 2Na 2H2O: Absent
L-Valine: Absent
Vitamins
Choline Chloride: 0.004
Folic Acid: 0.004
myo-Inositol: 0.0072
Niacinamide: 0.004
D-Pantothenic Acid Ca: 0.004
Pyridoxal•HCl: 0.004
Riboflavin: 0.0004
Thiamine HCl: 0.004
Other
D-Glucose: Absent
Phenol Red, Sodium: 0.0159
Pyruvic Acid, Sodium: 0.11
Calcium Chloride•2H2O: 0.265 g/Liter
Ferric Nitrate 9H2O: 0.0001 g/Liter
Magnesium Sulfate: 0.09767 g/Liter
Potassium Chloride: 0.4 g/Liter
Sodium Chloride: 6.4 g/Liter
Sodium Phosphate Monobasic: 0.109 g/Liter
Amino Acids
L-Arginine HCl: Absent
L-Cystine 2 HCl: Absent
L-Glutamine: Absent
Glycine: Absent
L-Histidine HCl H2O: Absent
L-Isoleucine: Absent
L-Leucine: Absent
L-Lysine HCl: Absent
L-Methionine: Absent
L-Phenylalanine: Absent
L-Serine: Absent
L-Threonine: Absent
L-Tryptophan: Absent
L-Tyrosine 2Na 2H2O: Absent
L-Valine: Absent
Vitamins
Choline Chloride: 0.004
Folic Acid: 0.004
myo-Inositol: 0.0072
Niacinamide: 0.004
D-Pantothenic Acid Ca: 0.004
Pyridoxal•HCl: 0.004
Riboflavin: 0.0004
Thiamine HCl: 0.004
Other
D-Glucose: Absent
Phenol Red, Sodium: 0.0159
Pyruvic Acid, Sodium: 0.11
Total
7.43 g/Liter
Preparation and Storage
Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.
Related Product Information for Dulbecco's MEM (DMEM) w/o Amino Acids, Glucose reagent
Dulbecco’s MEM is the most widely used modification of BME. It contains a 4-fold higher concentration of amino acids and vitamins. Non-essential amino acids and certain essential trace elements were added. The bicarbonate concentration was increased. The standard formula for DMEM is with 1000mg/ml glucose. DMEM was originally developed for the culture of mice embryonic cells. Today, it finds a broad application of serum free culture of normal and transformed mouse and chicken cells.
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Product Notes
The Dulbecco's MEM (DMEM) w/o Amino Acids, Glucose (Catalog #AAA6120661) is a Reagent and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Dulbecco's MEM (DMEM) w/o Amino Acids, Glucose, Reagent" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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