Cell Lysis Buffer Reagent
Cell Lysis Buffer (Component G) used for NAD/NADH, NADP/NADPH assay
Synonyms
Cell Lysis Buffer; Cell Lysis Buffer (Component G) used for NAD/NADH; NADP/NADPH assay; EliteTM Cell Lysis Buffer; Cell Lysis Buffer reagent
Protocol
1. Lysis of bacterial cells
Collecting bacterial cells by centrifugation ((10,000 x g, 0 degree C, 15 min). Use about 100 to 10 million cells/mL lysis buffer, leave at room temperature for 15 minutes. And then centrifuge at 2500 rpm for 5 minutes, use the supernatant for the assay.
2. Lysis of plant cells:
Homogenize the leave with the lysis buffer at 200 mg/mL, and then centrifuge at 2500 rpm for 5-10 minutes, use the supernatant for the assay.
3. Lysis of mammalian cells
Simply remove medium from the plates (wells), use about 100 uL lysis buffer per 1-5 million cells (or 100uL/ well in a 96-well cell culture plate), and leave at room temperature for 15 minutes. You can use the cell lysate directly or simply centrifuge at 1500 rpm for 5 minutes, use the supernatant for the assay.
4. Lysis of tissues.
Weight ~20 mg tissue, wash with cold PBS, homogenize with 400 mul of lysis buffer in a micro-centrifuge tube, and then centrifuge at 2500 rpm for 5-10 minutes, use the supernatant for the assay.
Collecting bacterial cells by centrifugation ((10,000 x g, 0 degree C, 15 min). Use about 100 to 10 million cells/mL lysis buffer, leave at room temperature for 15 minutes. And then centrifuge at 2500 rpm for 5 minutes, use the supernatant for the assay.
2. Lysis of plant cells:
Homogenize the leave with the lysis buffer at 200 mg/mL, and then centrifuge at 2500 rpm for 5-10 minutes, use the supernatant for the assay.
3. Lysis of mammalian cells
Simply remove medium from the plates (wells), use about 100 uL lysis buffer per 1-5 million cells (or 100uL/ well in a 96-well cell culture plate), and leave at room temperature for 15 minutes. You can use the cell lysate directly or simply centrifuge at 1500 rpm for 5 minutes, use the supernatant for the assay.
4. Lysis of tissues.
Weight ~20 mg tissue, wash with cold PBS, homogenize with 400 mul of lysis buffer in a micro-centrifuge tube, and then centrifuge at 2500 rpm for 5-10 minutes, use the supernatant for the assay.
Preparation and Storage
2-8 degree C.
Related Product Information for Cell Lysis Buffer reagent
Description: The EliteTM Cell Lysis Buffer is a ready-to-use lysis buffer for the preparation of cellular extracts from bacterial cells, plant cells, mammalian cells, and various tissues.
Product Categories/Family for Cell Lysis Buffer reagent
Similar Products
Product Notes
The Cell Lysis Buffer (Catalog #AAA433674) is a Reagent and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Cell Lysis Buffer, Reagent" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
