Retinoblastoma-associated protein (Rb1) Recombinant Protein | Rb1 recombinant protein

Recombinant Rat Retinoblastoma-associated protein (Rb1) , partial

• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: Retinoblastoma-associated protein (Rb1); Recombinant Rat Retinoblastoma-associated protein (Rb1); partial; Rb1 recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process)
• Sequence Length: 920

• Species: Rat
• Preparation and Storage: Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.

Related Product Information for Rb1 recombinant protein

This protein is a negative regulator of the cell cycle and was the first tumor suppressor gene found. The encoded protein also stabilizes constitutive heterochromatin to maintain the overall chromatin structure. The active, hypophosphorylated form of the protein binds transcription factor E2F1. Defects in this gene are a cause of childhood cancer retinoblastoma (RB), bladder cancer, and osteogenic sarcoma.

NCBI and Uniprot Product Information

• NCBI GI #: 281332095
• NCBI GeneID: 24708
• NCBI Accession #: NP_058741.1
• NCBI GenBank Nucleotide #: NM_017045.1
• UniProt Accession #: P33568; Q63527
• Molecular Weight: 105,025 Da
• NCBI Official Full Name: retinoblastoma-associated protein
• NCBI Official Synonym Full Names: RB transcriptional corepressor 1
• NCBI Official Symbol: Rb1
• NCBI Protein Information: retinoblastoma-associated protein
• UniProt Protein Name: Retinoblastoma-associated protein
• Protein Family: Retinoblastoma-associated protein
• UniProt Gene Name: Rb1
• UniProt Synonym Gene Names: Rb-1; Rb

NCBI Description

plays a role in negative regulation of the cell cycle and of DNA replication; acts as a tumor suppressor [RGD, Feb 2006]

Uniprot Description

Key regulator of entry into cell division that acts as a tumor suppressor. Promotes G0-G1 transition when phosphorylated by CDK3/cyclin-C. Acts as a transcription repressor of E2F1 target genes. The underphosphorylated, active form of RB1 interacts with E2F1 and represses its transcription activity, leading to cell cycle arrest. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV39H1, KMT5B and KMT5C, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Inhibits the intrinsic kinase activity of TAF1. Mediates transcriptional repression by SMARCA4/BRG1 by recruiting a histone deacetylase (HDAC) complex to the c-FOS promoter. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex.