Rat Forkhead Box Protein O1 (FOXO1) RTU ELISA Kit | FOXO1 rtu elisa kit

Rat Forkhead Box Protein O1 (FOXO1) ELISA Kit

Cat. Num. AAA459378

• Gene Names: Foxo1; Fkhr; Foxo1a
• Reactivity: Rat
• Synonyms: Forkhead Box Protein O1 (FOXO1); Rat Forkhead Box Protein O1 (FOXO1) ELISA Kit; FOX-O1; FKH1; FKHR; FOXO1A; Forkhead Homolog In Rhabdomyosarcoma; FOXO1 rtu elisa kit

• Reactivity: Rat
• Sequence Length: 649

• Assay Type: Sandwich
• Samples: Tissue Homogenates, Cell Lysates or Other Biological Fluids.
• Detection Range: 0.312-20ng/mL
• Sensitivity: 0.136ng/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for FOXO1 rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to FOXO1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to FOXO1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain FOXO1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of FOXO1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 300797651
• NCBI GeneID: 84482
• NCBI Accession #: NP_001178775.1
• NCBI GenBank Nucleotide #: NM_001191846.2
• UniProt Accession #: G3V7R4
• Molecular Weight: 69,344 Da
• NCBI Official Full Name: forkhead box protein O1
• NCBI Official Synonym Full Names: forkhead box O1
• NCBI Official Symbol: Foxo1
• NCBI Official Synonym Symbols: Fkhr; Foxo1a
• NCBI Protein Information: forkhead box protein O1
• UniProt Protein Name: Forkhead box protein O1
• Protein Family: Forkhead box protein
• UniProt Gene Name: Foxo1
• UniProt Synonym Gene Names: Foxo1a

NCBI Description

mediates effects of insulin on glucose-6-phosphatase gene expression [RGD, Feb 2006]

Uniprot Description

Transcription factor that is the main target of insulin signaling and regulates metabolic homeostasis in response to oxidative stress. Binds to the insulin response element (IRE) with consensus sequence 5'-TT[G/A]TTTTG-3' and the related Daf-16 family binding element (DBE) with consensus sequence 5'-TT[G/A]TTTAC-3'. Activity suppressed by insulin. Main regulator of redox balance and osteoblast numbers and controls bone mass. Orchestrates the endocrine function of the skeleton in regulating glucose metabolism. Acts synergistically with ATF4 to suppress osteocalcin/BGLAP activity, increasing glucose levels and triggering glucose intolerance and insulin insensitivity. Also suppresses the transcriptional activity of RUNX2, an upstream activator of osteocalcin/BGLAP. In hepatocytes, promotes gluconeogenesis by acting together with PPARGC1A and CEBPA to activate the expression of genes such as IGFBP1, G6PC and PCK1. Important regulator of cell death acting downstream of CDK1, PKB/AKT1 and STK4/MST1. Promotes neural cell death. Mediates insulin action on adipose tissue. Regulates the expression of adipogenic genes such as PPARG during preadipocyte differentiation and, adipocyte size and adipose tissue-specific gene expression in response to excessive calorie intake. Regulates the transcriptional activity of GADD45A and repair of nitric oxide-damaged DNA in beta-cells. Required for the autophagic cell death induction in response to starvation or oxidative stress in a transcription-independent manner. Mediates the function of MLIP in cardiomyocytes hypertrophy and cardiac remodeling (PubMed:26436652).