Rat Apoptotic Peptidase Activating Factor 1 (APAF1) RTU ELISA Kit | APAF1 rtu elisa kit

Rat Apoptotic Peptidase Activating Factor 1 (APAF1) ELISA Kit

Cat. Num. AAA457672

• Reactivity: Rat
• Synonyms: Apoptotic Peptidase Activating Factor 1 (APAF1); Rat Apoptotic Peptidase Activating Factor 1 (APAF1) ELISA Kit; CED4; Apoptotic Protease Activating Factor; APAF1 rtu elisa kit

• Reactivity: Rat
• Sequence Length: 1249

• Assay Type: Sandwich
• Samples: Tissue Homogenates, Cell Lysates or Other Biological Fluids.
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.062ng/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for APAF1 rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to APAF1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to APAF1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain APAF1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of APAF1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 13027436
• NCBI GeneID: 78963
• NCBI Accession #: NP_076469.1
• NCBI GenBank Nucleotide #: NM_023979.1
• UniProt Accession #: Q9EPV5
• Molecular Weight: 141,152 Da
• NCBI Official Full Name: apoptotic protease-activating factor 1
• NCBI Official Synonym Full Names: apoptotic peptidase activating factor 1
• NCBI Official Symbol: Apaf1
• NCBI Protein Information: apoptotic protease-activating factor 1
• UniProt Protein Name: Apoptotic protease-activating factor 1
• Protein Family: Apoptotic protease-activating factor
• UniProt Gene Name: Apaf1
• UniProt Synonym Gene Names: APAF-1

NCBI Description

protein involved in the initiation of apoptosis [RGD, Feb 2006]

Uniprot Description

Regulates progammed cell death; necessary for normal brain development. Participates with pro-caspase-9 (Apaf-3) in the cytochrome c-dependent activation of caspase-3, leading to apoptosis. This activation requires ATP ().