Rat Aconitase 1 (ACO1) RTU ELISA Kit | ACO1 rtu elisa kit

Rat Aconitase 1 (ACO1) ELISA Kit

Cat. Num. AAA457332

• Gene Names: Aco1; AH; IRP1; Acon1
• Reactivity: Rat
• Synonyms: Aconitase 1 (ACO1); Rat Aconitase 1 (ACO1) ELISA Kit; IREB1; IRP1; IREBP; Iron regulatory protein 1; Iron-responsive element-binding protein 1; Cytoplasmic aconitate hydratase; Citrate hydro-lyase; Ferritin repressor protein; ACO1 rtu elisa kit

• Reactivity: Rat
• Sequence Length: 889

• Assay Type: Sandwich
• Samples: Tissue Homogenates or Other Biological Fluids.
• Detection Range: 0.312-20ng/mL
• Sensitivity: 0.116ng/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ACO1 rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to ACO1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to ACO1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ACO1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of ACO1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 8394162
• NCBI GeneID: 50655
• NCBI Accession #: NP_059017.1
• NCBI GenBank Nucleotide #: NM_017321.1
• UniProt Accession #: Q63270
• Molecular Weight: 98,128 Da
• NCBI Official Full Name: cytoplasmic aconitate hydratase
• NCBI Official Synonym Full Names: aconitase 1
• NCBI Official Symbol: Aco1
• NCBI Official Synonym Symbols: AH; IRP1; Acon1
• NCBI Protein Information: cytoplasmic aconitate hydratase
• UniProt Protein Name: Cytoplasmic aconitate hydratase
• Protein Family: Aconitate hydratase
• UniProt Gene Name: Aco1
• UniProt Synonym Gene Names: Ireb1; Irebp; Aconitase; IRP1; IRE-BP 1

NCBI Description

This gene encodes a member of the aconitase/IPM isomerase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Depending on iron levels in the cytosol, the encoded protein can function as either an aconitase enzyme or as an mRNA binding protein. When cellular iron levels are high, the encoded protein functions as an aconitase, an essential enzyme in the TCA cycle that catalyzes the conversion of citrate to isocitrate. When cellular iron levels are low, the encoded protein regulates iron uptake and utilization by binding to iron-responsive elements in the untranslated regions of mRNAs for genes involved in iron metabolism [provided by RefSeq, Jan 2014]

Uniprot Description

Iron sensor. Binds a 4Fe-4S cluster and functions as aconitase when cellular iron levels are high. Functions as mRNA binding protein that regulates uptake, sequestration and utilization of iron when cellular iron levels are low. Binds to iron-responsive elements (IRES) in target mRNA species when iron levels are low. Binding of a 4Fe-4S cluster precludes RNA binding ().