Organic Anion Transporter 3, Rat Peptide | Slc22a8 peptide

Organic Anion Transporter 3, Rat (OAT3) (Control Peptide)

Cat. Num. AAA659674

• Gene Names: Slc22a8; OCT3; Oat3; Roct
• Applications: ELISA
• Purity: Highly Purified; Highly purified
• Synonyms: Organic Anion Transporter 3; Rat; Rat (OAT3) (Control Peptide); Slc22a8 peptide

• Host: Rat
• Purity/Purification: Highly Purified; Highly purified
• Form/Format: Supplied as a liquid in PBS, pH 7.5.

• Applicable Applications for Slc22a8 peptide: ELISA (EL/EIA)
• Application Notes: Suitable for use in ELISA, Antibody Blocking. Not suitable for use in Western Blot.; Dilution: ELISA: Coat ELISA plates at 1ug/ml of Control Peptide.; Antibody Blocking: 5-10ug Control Peptide per 1ug affintiy purified antibody (O3003-20) or per 1ul antiserum (O3003-21).
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for Slc22a8 peptide

Control Peptide for O3003-20 and O3003-21. No significant sequence homology is detected with other OATs or other proteins.

Mammalian kidney and liver are critical in maintaining physiological ionic environment. Kidney specializes in removing toxins, drugs, and other organic anions from the blood by a process called "renal secretion". Besides kidney, anionic substrates are also transported in other organs, e.g., choroid plexus, eye, airway, and placenta. Several multispecific OATs (OAT1-3, OAT-K1 and OATK2) and OATPs (organic anion transporting polypeptides; oatp1-3), have been cloned and characterized from various tissues. OATPs family of proteins are very similar in sequence and secondary protein structure (up to 12 transmembrane domains with cytoplasmic N- and C-terminus).
Recently OAT3 (rat 536-aa, human 568 aa) has been cloned that is most closely related to OAT1 (~49% identity). Rat OAT3 is expressed in the liver, brain, kidney and eye. OAT3 also mediates Na-independent uptake of several organic anions (PAH, ochratoxin, and estrone sulfate, etc). OAT3 shows 92% homology with mouse Roct (reduced in osteosclerosis transporter), possibly an ortholog of rat OAT3. As compared to OAT1-2, strong expression of OAT3 in brain suggests its role in removing organic anion in the brain.

Product Categories/Family for Slc22a8 peptide

Molecular Biology; MB-Ion Channel

NCBI and Uniprot Product Information

• NCBI GI #: 5545293
• NCBI GeneID: 83500
• UniProt Accession #: Q9R1U7; Q66HN0
• Molecular Weight: 59,186 Da
• NCBI Official Full Name: organic anion transporter 3
• NCBI Official Synonym Full Names: solute carrier family 22 (organic anion transporter), member 8
• NCBI Official Symbol: Slc22a8
• NCBI Official Synonym Symbols: OCT3; Oat3; Roct
• NCBI Protein Information: solute carrier family 22 member 8; rOat3; organic anion transporter 3; solute carrier family 22 ,member 8
• UniProt Protein Name: Solute carrier family 22 member 8
• Protein Family: Solute carrier family
• UniProt Gene Name: Slc22a8
• UniProt Synonym Gene Names: Oat3; rOat3
• UniProt Entry Name: S22A8_RAT

NCBI Description

organic anion transporter; involved in homovanillic acid (HVA), an end metabolite of dopamine, transport [RGD, Feb 2006]

Uniprot Description

Function: Plays an important role in the excretion/detoxification of endogenous and exogenous organic anions, especially from the brain and kidney. Functions as one of the detoxification systems on the choroid plexus (CP) by removing substrates from the cerebrospinal fluid (CSF). Functions as an organic anion/dicarboxylate exchanger that couples organic anion uptake indirectly to the sodium gradient. Mediates the uptake from brain of organic anions, such as p-aminohippurate (PAH), ochratoxin A, and estrone sulfate (ES) and a cationic compound, cimetidine in a sodium independent manner. Mediates the uptake of benzylpenicillin (PCG), 2,4-dichloro-phenoxyacetate (2,4-D) and PAH in CP. Mediates the efflux of PAH, pravastatin, pitavastatin and PCG from the cerebrum into the blood circulation across the blood-brain barrier (BBB). Involved in regulating the CSF concentration of H(2)-receptor antagonists at the CP. Mediates the renal uptake of indoxyl sulfate (IS), 3-carboxy-4-methyl-5-propyl-2-furanpropionate (CMPF) pravastatin, PCG, dehydroepiandrosterone sulfate (DHEAS) and is partly involved in the renal uptake of temocaprilat and ES. Ref.1 Ref.3 Ref.4 Ref.5 Ref.6 Ref.7 Ref.8 Ref.9 Ref.10 Ref.11

Subcellular location: Basolateral cell membrane; Multi-pass membrane protein

Potential. Note: Localizes on the brush border membrane of the choroid epithelial cells. Localizes to the basolateral membrane of the proximal tubular cells. Localizes on the abluminal and possibly, luminal membrane of the brain capillary endothelial cells (BCEC). Ref.3 Ref.4 Ref.6

Tissue specificity: Expressed in the liver, brain, kidney, choroid plexus and eye. Moderately expressed in the brain capillary endothelial cells (BCEC). Ref.1 Ref.3 Ref.6

Sequence similarities: Belongs to the major facilitator (TC 2.A.1) superfamily. Organic cation transporter (TC 2.A.1.19) family. [View classification]