Collagen Native Protein | COL4A6 native protein

Type I Rat Tendon Collagen-4 mg/ml

Cat. Num. AAA555886

• Gene Names: COL4A6; DFNX6; DELXq22.3; CXDELq22.3
• Applications: Cell Culturing System
• Synonyms: Collagen; Type I Rat Tendon Collagen-4 mg/ml; COL4A6 native protein

• Host: Rat
• Form/Format: Liquid
• Concentration: 4 mg/ml by Sicrol Assay (varies by lot)
• Sequence Length: 1678

• Applicable Applications for COL4A6 native protein: Cell Culturing System
• Source Note: Rat Tendon
• pH: 3
• Buffer: 0.2 M Acetic Acid
• Recommended Coating and Washing Volumes: 1. Add sufficient collagen solution to coat dishes, plates or inserts. 1-2 ml of solution is sufficient to cover a 35 mm dish. See below table for recommendations for different well sizes.; 2. Incubate at room temperature in a biological safety cabinet partially covered for 1 hour.; 3. Carefully aspirate remaining solution. Rinse with proper volume of serum-free media to remove buffer solution.; 4. Coated plates are best used immediately but can be air-dried and stored.; 5. Coated plates that have not been cultured can be dried and stored at 2 -8 degree C and are useful up to 1 week under sterile conditions; ; Wells: 6; Area(cm2): 4.67; Coating Volume (mL): 0.6; Wash Volume (mL): 1; Wells: 12; Area(cm2): 1.12; Coating Volume (mL): 0.25; Wash Volume (mL): 0.4; Wells: 24; Area(cm2): 0.33; Coating Volume (mL): 0.05; Wash Volume (mL): 0.1; Wells: 75mm insert; Area(cm2): 44; Coating Volume (mL): 5; Wash Volume (mL): 8; Wells: 96; Area(cm2): 0.143; Coating Volume (mL): 0.025; Wash Volume (mL): 0.05
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for COL4A6 native protein

Collagen naturally plays an important role within the body, but it is being used increasingly within certain areas of medicine as well. The breakdown of the body natural collagen can lead to a number of health problems, but the protein can also be used in other contexts to help with the repair of the body. Collagen comprises a family of genetically distinct molecules, all of which have a unique triple helix configuration of three polypeptide subunits known as alpha-chains. Each chain contains around 1000 amino acids, and usually features an amino acid sequence consisting of glycine, proline and hydroxyproline.

NCBI and Uniprot Product Information

• NCBI GI #: 466538
• NCBI GeneID: 1288
• Molecular Weight: 163,629 Da
• NCBI Official Full Name: collagen
• NCBI Official Synonym Full Names: collagen type IV alpha 6 chain
• NCBI Official Symbol: COL4A6
• NCBI Official Synonym Symbols: DFNX6; DELXq22.3; CXDELq22.3
• NCBI Protein Information: collagen alpha-6(IV) chain
• UniProt Protein Name: Collagen alpha-6(IV) chain
• Protein Family: Collagen
• UniProt Gene Name: COL4A6

NCBI Description

This gene encodes one of the six subunits of type IV collagen, the major structural component of basement membranes. Like the other members of the type IV collagen gene family, this gene is organized in a head-to-head conformation with another type IV collagen gene, alpha 5 type IV collagen, so that the gene pair shares a common promoter. Deletions in the alpha 5 gene that extend into the alpha 6 gene result in diffuse leiomyomatosis accompanying the X-linked Alport syndrome caused by the deletion in the alpha 5 gene. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Dec 2013]

Uniprot Description

Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen.