Rat anti-Mouse Foxp3 Antibody | anti-FOXP3 antibody

Rat Anti-Mouse Foxp3-FITC

Cat. Num. AAA670395

• Gene Names: FOXP3; JM2; AIID; IPEX; PIDX; XPID; DIETER
• Reactivity: Mouse
• Synonyms: Foxp3; Rat Anti-Mouse Foxp3-FITC; anti-FOXP3 antibody

• Host: Rat
• Reactivity: Mouse
• Isotype: IgG2a
• Clone Number: SB168a
• Specificity: Mouse Foxp3 (Mr ~57 kDa). Does not cross react with Human Foxp3. T regulatory cells (Originally called T suppressor cells) are a subpopulation of T cells. Their major roles are to suppress excess immune response of other cells and to maintain tolerance of self-antigens. Initially, researchers used CD4 and CD25 (IL-2 receptor) to identify T suppress subpopulation and purified CD4+CD25+ T cells were used for T cells Suppression Assay, however some other activated T cells also express CD25.1,2 The most convincible molecular marker of Treg cells is Forkhead box protein 3 (Foxp3) which is expressed in the nucleus.3 Anti-Foxp3 antibodies are now widely applied to identify the roles of Treg cells in the development of many diseases, such as cancer, autoimmune disease, and organ transplantation.4,5,6 Scientists found that the over-activation of Treg cells may cause cancer cells to escape from immune surveillance 5. Conversely, patients with autoimmune diseases such as psoriasis, systemic lupus erythematous (SLE) have relative dysfunction of Foxp3 positive cells.7,8 Immunologists are trying to induce immune tolerance in organ transplantation using Treg cells.6
• Form/Format: Fluorescein (FITC) Conjugate

• Application Notes: Flow Cytometry: < 1.0 ug/10^6 cells
• Immunogen: N-terminal recombinant Mouse Foxp3
• Characterization: To insure acceptable performance, each batch of product is tested by flow cytometry to conform to characteristics of a standard reference reagent. Representative flow data are included in this product insert
• Preparation and Storage: The fluorescein (FITC) and Alexa Fluor 647 (AF647) conjugates are supplied as 0.1 mg in 0.2 mL PBS/NaN3. Store at 2-8 degree C.

Product Categories/Family for anti-FOXP3 antibody

Epigenetics & Nuclear Staining

References

1. Fehérvari Z, Sakaguchi S. Development and function of CD25+CD4+ regulatory T cells. Curr Opin Immunol. 2004 Apr; 16(2):203-8. Review. 2. Gallimore A, Sakaguchi S. Regulation of tumor immunity by CD25+ T cells. Immunology. 2002 Sep; 107(1):5-9. Review. 3. Zhang L, Zhao Y (June 2007). "The regulation of Foxp3 expression in regulatory CD4(+)CD25(+)T cells: multiple pathways on the road". J. Cell. Physiol. 211 (3): 590-597. 4. Sakaguchi S, Sakaguchi N, Shimizu J, Yamazaki S, Sakihama T, Itoh M, Kuniyasu Y, Nomura T, Toda M, Takahashi T. Immunologic tolerance maintained by CD25+ CD4+ regulatory T cells: their common role in controlling autoimmunity, tumor immunity, and transplantation tolerance. Immunol Rev. 2001 Aug; 182:18-32 5. Elkord E, Alcantar-Orozco EM, Dovedi SJ, Tran DQ, Hawkins RE, Gilham DE. T regulatory cells in cancer: recent advances and therapeutic potential. Expert Opin Biol Ther. 2010 Nov; 10(11):1573-86. 6. FOXP3(+) regulatory T cells: From suppression of rejection to induction of renal allograft tolerance. Dummer CD, Carpio VN, Gonçalves LF, Manfro RC, Veronese FV. Transpl Immunol. 2011 Sep 13. 7. Horwitz DA.. Identity of mysterious CD4+CD25-Foxp3+ cells in SLE. Arthritis Res Ther 2010;12(1):101 8. Bovenschen HJ, van de Kerkhof PC, van Erp PE, Woestenenk R, Joosten I, Koenen HJ. Foxp3+ regulatory T cells of psoriasis patients easily differentiate into IL-17A-producing cells and are found in lesional skin. J Invest Dermatol. 2011 Sep; 131(9):1853-60

NCBI and Uniprot Product Information

• NCBI GI #: 167466190
• NCBI GeneID: 50943
• NCBI Accession #: NP_001107849.1
• NCBI GenBank Nucleotide #: NM_001114377.1
• UniProt Accession #: Q9BZS1; O60827; Q14DD8; Q4ZH51; A5HJT1; B7ZLG0; B9UN80
• Molecular Weight: 47,244 Da
• NCBI Official Full Name: forkhead box protein P3 isoform b
• NCBI Official Synonym Full Names: forkhead box P3
• NCBI Official Symbol: FOXP3
• NCBI Official Synonym Symbols: JM2; AIID; IPEX; PIDX; XPID; DIETER
• NCBI Protein Information: forkhead box protein P3; scurfin; FOXP3delta7; immunodeficiency, polyendocrinopathy, enteropathy, X-linked; immune dysregulation, polyendocrinopathy, enteropathy, X-linked
• UniProt Protein Name: Forkhead box protein P3
• Protein Family: Forkhead box protein
• UniProt Gene Name: FOXP3
• UniProt Synonym Gene Names: IPEX
• UniProt Entry Name: FOXP3_HUMAN

NCBI Description

The protein encoded by this gene is a member of the forkhead/winged-helix family of transcriptional regulators. Defects in this gene are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX), also known as X-linked autoimmunity-immunodeficiency syndrome. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Probable transcription factor. Plays a critical role in the control of immune response.

Subunit structure: Interacts with IKZF3. Ref.8

Subcellular location: Nucleus

Potential.

Post-translational modification: Acetylation on lysine residues stabilizes FOXP3 and promotes differentiation of T-cells into induced regulatory T-cells (iTregs) associated with suppressive functions. Deacetylated by SIRT1.

Involvement in disease: Immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX) [MIM:304790]: Characterized by neonatal onset insulin-dependent diabetes mellitus, infections, secretory diarrhea, thrombocytopenia, anemia and eczema. It is usually lethal in infancy.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.10 Ref.11 Ref.12 Ref.13

Sequence similarities: Contains 1 C2H2-type zinc finger.Contains 1 fork-head DNA-binding domain.