GLUT 2, Rat Peptide | Gls peptide
GLUT 2, Rat (Glucose Transporter, Insulin Regulatable) (Control Peptide)
Gene Names
Gls; Glut; RATGLUT
Applications
ELISA
Purity
Highly PurifiedHighly purified
Synonyms
GLUT 2; Rat; Rat (Glucose Transporter; Insulin Regulatable) (Control Peptide); Gls peptide
Host
Rat liver plasma membranes
Purity/Purification
Highly Purified
Highly purified
Highly purified
Form/Format
Supplied as a liquid in PBS, pH 7.2
Applicable Applications for Gls peptide
ELISA (EL/EIA)
Application Notes
Suitable for use in ELISA, Antibody Blocking.
Dilution: ELISA: 1ug/ml.
Antibody Blocking: 5-10ug per 1ug G3900-15D (affinity purified antibody) or per 1ul G3900-15 (antiserum).
Dilution: ELISA: 1ug/ml.
Antibody Blocking: 5-10ug per 1ug G3900-15D (affinity purified antibody) or per 1ul G3900-15 (antiserum).
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for Gls peptide
Control Peptide for G3900-15D (affinity purified antibody) and G3900-15 (antiserum). G3900-15F is 16 amino acid peptide sequence corresponding to the exoplasmic loop between helices 1 and 2 of rat liver Glucose Transporter. Rat GT21 peptide sequence has 93% identity with mouse and human Glut-2 (15/16 AA).
Most mammalian cells transport glucose through a family of membrane proteins known as glucose transporters. Molecular cloning of these glucose transporters has identified a family of closely related genes that encodes at least 7 proteins (Glut-1 to Glut-7, 40-60kD) and sodium glucose co-transporter-1 (SGLT-1, 662 amino acids; ~75kD). Individual member of this family have identical predicted secondary structures with 12 transmembrane domains. Both N and C-termini are predicted to be cytoplasmic. Most differences in sequence homology exist within the four hydrophilic domains that may play a role in tissue-specific targeting. Glut isoforms differ in their tissue expression, substrate specificity and kinetic characteristics. Glut-1 mediates glucose transport into red cells, throughout the blood brain barrier, and to most cells. Glut-2 provides glucose to the liver and pancreatic cells. Glut-3 is the main transporter in neurons. Glut-4 is primarily expressed in muscle and adipose tissue and regulated by insulin. Glut-5 transports fructose in intestine and testis. Glut-6 is a pseudogene and unlikely to be expressed at the protein level. Glut-7, expressed in liver and other gluconeogenic tissues, mediates glucose flux across endoplasmic reticulum membrane. Glut-8 is found in adult testis and placenta. Human Glut-9 is expressed in spleen, peripheral leukocytes and brain. Human Glut-10 (541aa, chromosome 20q13.1; ~30-35% homology with Glut-3 and Glut-8) has been identified as a candidate gene for NIDDM susceptibility. It is widely expressed with highest levels in liver and pancreas. Glut-11 (496aa, chromosome 22q11.2; ~41% identity with Glut-5) is expressed in heart and skeletal muscle. Glut-12 (human 617aa,, monkey 621aa;~ 50 kD; ~30% homology with Glut-4 and 40% with Glut-10) is expressed in skeletal muscle, adipose tissue and small intestine. Glut-13 or proton myo-inositol transporter (HMIT; human 629aa, rat 618aa, ~75-90 kD/67 kD protein) is highly expressed in glial cells and some neurons. Glut-13 transport activity was specific for myo-inositol. Rat HMIT is ~35% identical to rat GlutX1.
Most mammalian cells transport glucose through a family of membrane proteins known as glucose transporters. Molecular cloning of these glucose transporters has identified a family of closely related genes that encodes at least 7 proteins (Glut-1 to Glut-7, 40-60kD) and sodium glucose co-transporter-1 (SGLT-1, 662 amino acids; ~75kD). Individual member of this family have identical predicted secondary structures with 12 transmembrane domains. Both N and C-termini are predicted to be cytoplasmic. Most differences in sequence homology exist within the four hydrophilic domains that may play a role in tissue-specific targeting. Glut isoforms differ in their tissue expression, substrate specificity and kinetic characteristics. Glut-1 mediates glucose transport into red cells, throughout the blood brain barrier, and to most cells. Glut-2 provides glucose to the liver and pancreatic cells. Glut-3 is the main transporter in neurons. Glut-4 is primarily expressed in muscle and adipose tissue and regulated by insulin. Glut-5 transports fructose in intestine and testis. Glut-6 is a pseudogene and unlikely to be expressed at the protein level. Glut-7, expressed in liver and other gluconeogenic tissues, mediates glucose flux across endoplasmic reticulum membrane. Glut-8 is found in adult testis and placenta. Human Glut-9 is expressed in spleen, peripheral leukocytes and brain. Human Glut-10 (541aa, chromosome 20q13.1; ~30-35% homology with Glut-3 and Glut-8) has been identified as a candidate gene for NIDDM susceptibility. It is widely expressed with highest levels in liver and pancreas. Glut-11 (496aa, chromosome 22q11.2; ~41% identity with Glut-5) is expressed in heart and skeletal muscle. Glut-12 (human 617aa,, monkey 621aa;~ 50 kD; ~30% homology with Glut-4 and 40% with Glut-10) is expressed in skeletal muscle, adipose tissue and small intestine. Glut-13 or proton myo-inositol transporter (HMIT; human 629aa, rat 618aa, ~75-90 kD/67 kD protein) is highly expressed in glial cells and some neurons. Glut-13 transport activity was specific for myo-inositol. Rat HMIT is ~35% identical to rat GlutX1.
Product Categories/Family for Gls peptide
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
74,024 Da
NCBI Official Full Name
glutaminase kidney isoform, mitochondrial isoform a
NCBI Official Synonym Full Names
glutaminase
NCBI Official Symbol
Gls
NCBI Official Synonym Symbols
Glut; RATGLUT
NCBI Protein Information
glutaminase kidney isoform, mitochondrial; K-glutaminase; L-glutamine amidohydrolase
UniProt Protein Name
Glutaminase kidney isoform, mitochondrial
Protein Family
UniProt Gene Name
Gls
UniProt Synonym Gene Names
GLS
UniProt Entry Name
GLSK_RAT
NCBI Description
catalyzes the conversion of L-glutamine and H2O to L-glutamate and NH3 in glutamine catabolism [RGD, Feb 2006]
Uniprot Description
Function: Catalyzes the first reaction in the primary pathway for the renal catabolism of glutamine. Plays a role in maintaining acid-base homeostasis. Regulates the levels of the neurotransmitter glutamate in the brain.
Catalytic activity: L-glutamine + H2O = L-glutamate + NH3.
Subunit structure: Homotetramer. Tetramer composed of 68 and 65 kDa peptides in a 1:3 ratio. Interacts with ATCAY; the interaction is direct and may control GLS localization, negatively regulating its activity. Ref.6
Subcellular location: Isoform 1: Cytoplasm › cytosol
By similarity. Isoform 2: Mitochondrion.
Tissue specificity: Kidney, brain, and intestine.
Sequence similarities: Belongs to the glutaminase family.Contains 2 ANK repeats.
Research Articles on Gls
Similar Products
Product Notes
The Gls gls (Catalog #AAA659335) is a Peptide produced from Rat liver plasma membranes and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's GLUT 2, Rat can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA). Suitable for use in ELISA, Antibody Blocking. Dilution: ELISA: 1ug/ml. Antibody Blocking: 5-10ug per 1ug G3900-15D (affinity purified antibody) or per 1ul G3900-15 (antiserum). Researchers should empirically determine the suitability of the Gls gls for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GLUT 2, Rat, Peptide" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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