Rat Von Willebrand Factor (vWF) ELISA Kit | vWF elisa kit

Rat Von Willebrand Factor (vWF) ELISA Kit

Cat. Num. AAA453668

• Reactivity: Rat
• Synonyms: Von Willebrand Factor (vWF); Rat Von Willebrand Factor (vWF) ELISA Kit; F8VWF; VWD; von Willebrand antigen 2; vWF elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of VWF. No significant cross-reactivity or interference between VWF and analogues was observed.
• Sequence Length: 430

• Samples: Plasma.
• Assay Type: Competitive inhibition
• Detection Range: 15.625-1000ng/mL
• Sensitivity: 6.6ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level VWF were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level VWF were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for vWF elisa kit

Intended Uses: The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of VWF in rat plasma

Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to VWF has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled VWF and unlabeled VWF (Standards or samples) with the pre-coated antibody specific to VWF. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of VWF in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of VWF in the sample.

NCBI and Uniprot Product Information

• NCBI GI #: 56404678
• NCBI GeneID: 116669
• UniProt Accession #: Q62935; Q78E31; Q9Z0P2
• Molecular Weight: 48,139 Da
• NCBI Official Full Name: von Willebrand factor
• NCBI Official Synonym Full Names: von Willebrand factor
• NCBI Official Symbol: Vwf
• NCBI Protein Information: von Willebrand factor
• UniProt Protein Name: von Willebrand factor
• Protein Family: von Willebrand factor
• UniProt Gene Name: Vwf
• UniProt Synonym Gene Names: vWF

NCBI Description

human homolog is a plasma glycoprotein that mediates platelet adhesion to damaged blood vessels and stabilizes blood coagulation factor VIII [RGD, Feb 2006]

Uniprot Description

Important in the maintenance of hemostasis, it promotes adhesion of platelets to the sites of vascular injury by forming a molecular bridge between sub-endothelial collagen matrix and platelet-surface receptor complex GPIb-IX-V. Also acts as a chaperone for coagulation factor VIII, delivering it to the site of injury, stabilizing its heterodimeric structure and protecting it from premature clearance from plasma ().