Rat Vacuolar protein sorting-associated protein 29 (VPS29) ELISA Kit | VPS29 elisa kit

Rat Vacuolar protein sorting-associated protein 29 (VPS29) ELISA Kit

Cat. Num. AAA286706

• Reactivity: Rat
• Synonyms: Vacuolar protein sorting-associated protein 29 (VPS29); Rat Vacuolar protein sorting-associated protein 29 (VPS29) ELISA Kit; DC15; DC7; DKFZp564F0223; FLJ20492; PEP11; retromer protein; vacuolar protein sorting 29; vacuolar sorting protein VPS29/PEP11; x 007 protein; VPS29 elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Rat VPS29. No significant cross-reactivity or interference between Rat VPS29 and analogues was observed.
• Sequence Length: 182

• Assay Type: Quantitive Sandwich
• Samples: Serum, Plasma, Other Biological Fluids
• Detection Wavelength: 450 nm
• Intra-assay Precision (Precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
• Inter-assay Precision (Precision between assays): Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
• CV (%): = SD/meanX100
• Intra-Assay: CV
• Inter-Assay: CV
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for VPS29 elisa kit

This assay employs a two-site sandwich ELISA to quantitate VPS29 in samples. An antibody specific for VPS29 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any VPS29 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for VPS29 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of VPS29 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 157786944
• NCBI GeneID: 288666
• NCBI Accession #: NP_001099402.1
• NCBI GenBank Nucleotide #: NM_001105932.1
• UniProt Accession #: B2RZ78; A6J1A4
• Molecular Weight: 20,890 Da
• NCBI Official Full Name: vacuolar protein sorting-associated protein 29
• NCBI Official Synonym Full Names: VPS29 retromer complex component
• NCBI Official Symbol: Vps29
• NCBI Protein Information: vacuolar protein sorting-associated protein 29
• UniProt Protein Name: Vacuolar protein sorting-associated protein 29
• Protein Family: Vacuolar protein sorting-associated protein
• UniProt Gene Name: Vps29

Uniprot Description

Acts as component of the retromer cargo-selective complex (CSC). The CSC is believed to be the core functional component of retromer or respective retromer complex variants acting to prevent missorting of selected transmembrane cargo proteins into the lysosomal degradation pathway. The recruitment of the CSC to the endosomal membrane involves RAB7A and SNX3. The SNX-BAR retromer mediates retrograde transport of cargo proteins from endosomes to the trans-Golgi network (TGN) and is involved in endosome-to-plasma membrane transport for cargo protein recycling. The SNX3-retromer mediates the retrograde endosome-to-TGN transport of WLS distinct from the SNX-BAR retromer pathway. The SNX27-retromer is believed to be involved in endosome-to-plasma membrane trafficking and recycling of a broad spectrum of cargo proteins. The CSC seems to act as recruitment hub for other proteins, such as the WASH complex and TBC1D5. Required to regulate transcytosis of the polymeric immunoglobulin receptor (pIgR-pIgA). Involved in GLUT1 endosome-to-plasma membrane trafficking; the function is dependent of association with ANKRD27 ().