Rat Uncoupling Protein 3 (UCP3) ELISA Kit | UCP3 elisa kit

Rat Uncoupling Protein 3 (UCP3) ELISA Kit

Cat. Num. AAA9360690

• Gene Names: UCP3; SLC25A9
• Reactivity: Rat
• Synonyms: Uncoupling Protein 3 (UCP3); Rat Uncoupling Protein 3 (UCP3) ELISA Kit; UCP3 elisa kit

• Reactivity: Rat

• Assay Type: Quantitative Sandwich
• Detection Range: 62.5pg/ml-2000pg/ml
• Sensitivity: 10pg/ml
• Intra-assay Precision: Intra-assay CV (%)is less than 15%.
• Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]. All CV% should be compared by concentration, not compared by OD values.
• Preparation and Storage: Store all reagents at 2-8 degree C

Related Product Information for UCP3 elisa kit

Background/Introduction: This Quantitative Sandwich ELISA kit is for lab reagent/research use only, not for drug, household, therapeutic or test applications! This kit is intended to be used for determine the level of UCP3 (hereafter termed "analyte") in undiluted original Rat serum, plasma or tissue homogenates samples. For other sample types please contact tech support to determine compatibility with this assay. This kit is not suitable for assaying non-biological sources of substances.

NCBI and Uniprot Product Information

• NCBI GI #: 3176760
• NCBI GeneID: 7352
• Molecular Weight: 22,874 Da
• NCBI Official Full Name: uncoupling protein 3
• NCBI Official Synonym Full Names: uncoupling protein 3 (mitochondrial, proton carrier)
• NCBI Official Symbol: UCP3
• NCBI Official Synonym Symbols: SLC25A9
• NCBI Protein Information: mitochondrial uncoupling protein 3
• UniProt Protein Name: Mitochondrial uncoupling protein 3
• Protein Family: UBA domain-containing protein
• UniProt Gene Name: UCP3
• UniProt Synonym Gene Names: SLC25A9; UCP 3
• UniProt Entry Name: UCP3_HUMAN

NCBI Description

Mitochondrial uncoupling proteins (UCP) are members of the larger family of mitochondrial anion carrier proteins (MACP). UCPs separate oxidative phosphorylation from ATP synthesis with energy dissipated as heat, also referred to as the mitochondrial proton leak. UCPs facilitate the transfer of anions from the inner to the outer mitochondrial membrane and the return transfer of protons from the outer to the inner mitochondrial membrane. They also reduce the mitochondrial membrane potential in mammalian cells. The different UCPs have tissue-specific expression; this gene is primarily expressed in skeletal muscle. This gene's protein product is postulated to protect mitochondria against lipid-induced oxidative stress. Expression levels of this gene increase when fatty acid supplies to mitochondria exceed their oxidation capacity and the protein enables the export of fatty acids from mitochondria. UCPs contain the three solcar protein domains typically found in MACPs. Two splice variants have been found for this gene.[provided by RefSeq, Nov 2008]

Uniprot Description

UCP3: UCP are mitochondrial transporter proteins that create proton leaks across the inner mitochondrial membrane, thus uncoupling oxidative phosphorylation. As a result, energy is dissipated in the form of heat. May play a role in the modulation of tissue respiratory control. Participates in thermogenesis and energy balance. Defects in UCP3 may be involved in obesity (OBESITY). It is a condition characterized by an increase of body weight beyond the limitation of skeletal and physical requirements, as the result of excessive accumulation of body fat. Belongs to the mitochondrial carrier family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Transporter; Mitochondrial; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 11q13.4

Cellular Component: integral to membrane; mitochondrial inner membrane; mitochondrion

Molecular Function: oxidative phosphorylation uncoupler activity; protein binding; structural constituent of ribosome; transporter activity

Biological Process: aging; cellular metabolic process; cellular response to hormone stimulus; fatty acid metabolic process; lipid metabolic process; mitochondrial transport; proton transport; respiratory gaseous exchange; response to activity; response to cold; response to glucocorticoid stimulus; response to hypoxia; response to insulin stimulus; response to nutrient; response to superoxide; translation

Disease: Obesity