Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Transforming growth factor alpha (TGF-alpha) Standard Curve.)
Rat Transforming Growth Factor alpha (TGF-alpha) ELISA Kit | TGF-alpha elisa kit
Rat Transforming Growth Factor alpha (TGF-alpha) ELISA Kit
Reactivity
Rat
Synonyms
Transforming Growth Factor alpha (TGF-alpha); Rat Transforming Growth Factor alpha (TGF-alpha) ELISA Kit; TGFA; TFGA; transforming growth factor-alpha; TGF-alpha elisa kit
Reactivity
Rat
Specificity
Rat Transforming growth factor alpha (TGF-alpha) ELISA Kit has high sensitivity and excellent specificity for detection of Rat TGFA. No significant cross-reactivity or interference between Rat TGFA and analogues was observed.
Sequence Length
50
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Transforming growth factor alpha (TGF-alpha) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Transforming growth factor alpha (TGF-alpha) Standard Curve.)
Related Product Information for TGF-alpha elisa kit
Description: This Rat Transforming growth factor alpha (TGF-alpha) ELISA Kit employs a two-site sandwich ELISA to quantitate TGFA in samples. An antibody specific for TGFA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TGFA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TGFA is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TGFA bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: TGF-alpha gene encodes a growth factor that is a ligand for the epidermal growth factor receptor, which activates a signaling pathway for cell proliferation, differentiation and development. This protein may act as either a transmembrane-bound ligand or a soluble ligand. This gene has been associated with many types of cancers, and it may also be involved in some cases of cleft lip/palate.
Background: TGF-alpha gene encodes a growth factor that is a ligand for the epidermal growth factor receptor, which activates a signaling pathway for cell proliferation, differentiation and development. This protein may act as either a transmembrane-bound ligand or a soluble ligand. This gene has been associated with many types of cancers, and it may also be involved in some cases of cleft lip/palate.
NCBI and Uniprot Product Information
NCBI GI #
UniProt Accession #
NCBI Official Full Name
Transforming growth factor alpha
UniProt Protein Name
Transforming growth factor alpha
Protein Family
UniProt Gene Name
TGFA
UniProt Synonym Gene Names
TGF-alpha; ETGF
Uniprot Description
TGF alpha is a mitogenic polypeptide that is able to bind to the EGF receptor/EGFR and to act synergistically with TGF beta to promote anchorage-independent cell proliferation in soft agar (). Inhibitor of acid secretion. Inhibitor of aminopyrine uptake in parietal cells (in vitro).
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Product Notes
The Rat TGF-alpha tgfa (Catalog #AAA9711821) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9711821 ELISA Kit recognizes Rat TGF-alpha. It is sometimes possible for the material contained within the vial of "Transforming Growth Factor alpha (TGF-alpha), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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