Rat Toll Like Receptor 2 (TLR2) ELISA Kit | TLR2 elisa kit

Rat Toll Like Receptor 2 (TLR2) ELISA Kit

Cat. Num. AAA2022789

• Gene Names: TLR2; TIL4; CD282
• Reactivity: Rat
• Synonyms: Toll Like Receptor 2 (TLR2); Rat Toll Like Receptor 2 (TLR2) ELISA Kit; CD282; TIL4; Toll/interleukin-1 receptor-like protein 4; TLR2 elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of TLR2. No significant cross-reactivity or interference between TLR2 and analogues was observed.
• Sequence Length: 784

• Samples: rat tissue homogenates, cell lysates and other biological fluids
• Detection Range: 0.312-20ng/mL
• Sensitivity: <0.119ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level TLR2 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level TLR2 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TLR2 elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of TLR2 in rat tissue homogenates, cell lysates and other biological fluids.

Product Categories/Family for TLR2 elisa kit

Tumor immunity; Infection immunity; Immune molecule; Pulmonology

NCBI and Uniprot Product Information

• NCBI GI #: 20140434
• NCBI GeneID: 7097
• Molecular Weight: 89,838 Da
• NCBI Official Full Name: Toll-like receptor 2
• NCBI Official Synonym Full Names: toll-like receptor 2
• NCBI Official Symbol: TLR2
• NCBI Official Synonym Symbols: TIL4; CD282
• NCBI Protein Information: toll-like receptor 2
• UniProt Protein Name: Toll-like receptor 2
• Protein Family: Toll-like receptor
• UniProt Gene Name: TLR2
• UniProt Synonym Gene Names: TIL4
• UniProt Entry Name: TLR2_HUMAN

NCBI Description

The protein encoded by this gene is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This gene is expressed most abundantly in peripheral blood leukocytes, and mediates host response to Gram-positive bacteria and yeast via stimulation of NF-kappaB. [provided by RefSeq, Jul 2008]

Uniprot Description

TLR2: Cooperates with LY96 to mediate the innate immune response to bacterial lipoproteins and other microbial cell wall components. Cooperates with TLR1 to mediate the innate immune response to bacterial lipoproteins or lipopeptides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. May also promote apoptosis in response to lipoproteins. Recognizes mycoplasmal macrophage- activating lipopeptide-2kD (MALP-2), soluble tuberculosis factor (STF), phenol-soluble modulin (PSM) and B.burgdorferi outer surface protein A lipoprotein (OspA-L) cooperatively with TLR6. Interacts with LY96, TLR1 and TLR6 (via extracellular domain). Binds MYD88 (via TIR domain). Interacts with TICAM1. Ligand binding induces the formation of a heterodimer with TLR1. Interacts with CNPY3. Highly expressed in peripheral blood leukocytes, in particular in monocytes, in bone marrow, lymph node and in spleen. Also detected in lung and in fetal liver. Levels are low in other tissues. Belongs to the Toll-like receptor family.

Protein type: Membrane protein, integral; Cell surface; Receptor, misc.; Apoptosis; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 4q32

Cellular Component: cell projection; cell surface; integral to plasma membrane; cytoplasm; plasma membrane; external side of plasma membrane

Molecular Function: peptidoglycan binding; protein binding; transmembrane receptor activity; triacylated lipoprotein binding; protein heterodimerization activity; lipopolysaccharide receptor activity; receptor activity; pattern recognition receptor activity; diacylated lipoprotein binding

Biological Process: positive regulation of nitric oxide biosynthetic process; response to toxin; positive regulation of leukocyte migration; positive regulation of NF-kappaB import into nucleus; leukotriene metabolic process; detection of triacylated bacterial lipoprotein; positive regulation of interleukin-10 production; activation of NF-kappaB transcription factor; positive regulation of interferon-beta production; positive regulation of oligodendrocyte differentiation; toll-like receptor 4 signaling pathway; negative regulation of interleukin-12 production; detection of diacylated bacterial lipoprotein; cell surface pattern recognition receptor signaling pathway; positive regulation of interleukin-6 production; positive regulation of tumor necrosis factor production; positive regulation of chemokine production; toll-like receptor 2 signaling pathway; defense response to Gram-positive bacterium; myelin formation in the central nervous system; positive regulation of transcription from RNA polymerase II promoter; response to progesterone stimulus; I-kappaB phosphorylation; positive regulation of nitric-oxide synthase biosynthetic process; positive regulation of interleukin-12 production; apoptosis; microglial cell activation; response to molecule of fungal origin; signal transduction; positive regulation of interleukin-18 production; nitric oxide metabolic process; response to insulin stimulus; positive regulation of interleukin-8 production; negative regulation of cell proliferation; lipopolysaccharide-mediated signaling pathway; inflammatory response; positive regulation of Wnt receptor signaling pathway; positive regulation of tumor necrosis factor biosynthetic process; positive regulation of toll-like receptor signaling pathway; MyD88-dependent toll-like receptor signaling pathway; induction by symbiont of defense-related host nitric oxide production; negative regulation of interleukin-17 production; response to hypoxia; toll-like receptor signaling pathway; innate immune response; immune response; chloramphenicol transport; positive regulation of cytokine secretion; positive regulation of inflammatory response

Disease: Leprosy, Susceptibility To, 3; Mycobacterium Tuberculosis, Susceptibility To